Application Notes

Typical chiral stationary phase (CSP) screening paradigms utilize a single alcohol component as co-solvent in its mobile phase. Recent unexpected observations have demonstrated that mixed-alcohol mobile phases can enhance or even introduce peak resolution when none existed in a mono-alcohol system.

Insulin was the first recombinant pharmaceutical produced. Currently, one of the critical quality control attributes of therapeutic insulin in the US and European Pharmacopoeia (USP and EP) monograph is the level of covalent high molecular weight (HMW) insulin as determined by HPLC size-exclusion chromatography.

Malaria remains one of the world's most deadly diseases, killing a child under five years of age every 30 s. Our branch aims to produce and characterize recombinant protein vaccines for Phase I and II human trials.

Ion exchange is a perfect technique for analyzing large biomolecules such as proteins, peptides and oligonucleotides. These complex compounds contain numerous components (amino acids, nucleotides, glycans etc.) many of which possess a charged side chain or backbone.

Selective cleavage of proteins by the enzyme trypsin helps characterize larger proteins. While MS provides structural information, quantitative analysis is often based on detection by UV at 214 nm.

When working with complex matrices such as personal care products, sample preparation is perhaps the most difficult step of the analysis process. By utilizing a targeted sample preparation technique, such as ion-exchange SPE, analysis can be significantly improved as compared to less targeted techniques such as liquid-liquid extraction.

Compounds such as ibuprofen, chlorpheniramine, and allantoin are important in the field of pharmaceuticals and cosmetics. The analysis of acidic and basic compounds is particularly important but it is sometimes difficult to analyze both acidic and basic compounds on a single standard C18 column. Imtakt's Scherzo SM-C18 and Scherzo SW-C18 HPLC columns can separate acidic ibuprofen and basic chlorpheniramine simultaneously.

Resistive glass tubes and plates are designed to guide ions by generating a uniform electric field. Photonis resistive glass products are composed of a proprietary lead silicate glass that has been specially processed to create a resistive layer at the surface. The resistivity can be varied over several orders of magnitude to suit the specific application.

This application note demonstrates the use of the Thermo Scientific Syncronis C18 1.7 ?m column for the determination of esomeprazole by UHPLC-UV.

Plant materials contain a wealth of information on plant viability and health. The analysis of the metabolite contents of the leaves of various plants has been informative in the comparison of organisms.

This application note demonstrates the use of the Thermo Scientific Accucore RP-MS column for the determination of telmisartan by HPLC-UV.

Beta blockers can be abused in sports involving little physical activity, such as archery, to reduce cardiac contraction, heart rate, and coronary blood flow.

The migration to chromatographic methods utilizing smaller particle sizes has been well demonstrated to increase productivity through faster, more efficient separations.

As analytical chemist(s), the data collected from samples we test represent information. That information may be required to direct environmental clean-up operations, help insure worker safety, or guide decisions for an emergency response to a chemical incident.

Positron emission tomography (PET) is one of the most powerful noninvasive diagnostic tools for tracing organ functioning. Quality control of the short-lived radiopharmaceuticals is challenging, not at least because of the tough time limits, the radiation issue, and the near nanomole radiotracer quantities.

Red wine is a challenging matrix that contains organic acids, sugars, phenols, and pigments such as anthocyanins. This application note describes a simple, fast, novel, and effective method to clean up red wine co-extractives.

Shale gas reservoirs, such as the Marcellus shale reserve in Pennsylvania and Barnett shale reserve in Texas are a growing source of natural gas in the United States. Hydraulic fracturing or "fracking" involves pumping water, sand, and chemicals at extremely high pressure into deep underground wells to crack open hydrocarbon-rich shale formations and extract natural gas.

An effective analytical method for detecting pesticide residues in olive oil

Triple quadrupole LC/MS analysis of aflatoxins in food samples eliminates the need for expensive immuno-affinity cleanup columns and reduces the probability of false positives to almost zero.

The resolution, sensitivity, and mass accuracy of the Agilent 7200 GC/Q-TOF system provide rapid, simple, and reliable analysis of trace levels of sulfur compounds in coffee.

The automated derivatization of fatty acids (FAs) was performed with the Agilent 7696A Sample Prep WorkBench. Since free fatty acids show tailing in gas chromatography, transformation of fatty acids into fatty acid methyl esters (FAMEs) is widely used.

This application note describes the use of triggered Multiple Reaction Monitoring (tMRM) for the analysis of pesticide residues applied to chamomile and ginger extracts. The analysis is performed using the Agilent 1290 LC system coupled to a 6460 Triple Quadrupole LC/MS with tMRM acquisition.

Polychlorinated dibenzo-p-dioxins (PCDD) and polychlorinated dibenzofurans (PCDF) are fat-soluble, highly toxic, ubiquitous environmental contaminants found at trace levels in all foodstuffs and animal feed.

Chemical contaminants that are released into the marine environment may be ingested (absorbed) by fish and shellfish and thus become introduced into the human food chain.

Wine is a beverage produced and consumed throughout the world and is a highly valued commodity. Its classification and authenticity can be very important.

Whether the goal is food safety, ensuring quality or establishing provenance, measuring the trace element content of foods and beverages that we all consume is of paramount importance.

More than 1000 pesticides are currently used worldwide in the treatment of soil and crops. Many countries have established allowable levels of those pesticides in food to protect consumers. While these maximum residue levels (MRLs) vary, the default tolerance is 10 parts per billion (ppb).

A GC–MS analyser is described that is reported to substantially expand the workable application range of a classic catalyst contaminants analyser. The use of FS/SIM mode permits identification of unknown contaminants in combination with reliable quantification at trace and ultratrace amounts.

Phenomenex Application Note

Describes the preparation of a reversed-phase monolithic column with an optimized porous structure to separate intact proteins using LC–MS.