Application Notes

Sample preparation for multiresidue pesticide screening for agriculture commodities can be cumbersome and laborious for multiple sample extractions. With recent advances in multiresidue pesticide screening, methods have been simplified by the introduction of QuEChERS.

Current methods to measure worker exposure to chemicals generally involve collecting samples and sending them to a laboratory for analysis, which can take up to two weeks to obtain results. Tools that can turn samples into on-site information can provide data to make informed decisions when and where they are needed to protect workers.

Tosoh Bioscience LLC

Horizon Technology, Inc.

Imtakt USA

Caramel colorings are used as additives in a broad range of food and beverage products, but have no nutritional or preservative function. Recently, the potential hazard to humans of ammonia- and ammonia-sulfite-process caramel colorings was raised, because they contain the by-product 4-methylimidazole, which is a potential carcinogen (1).

Shodex/Showa Denko America Inc.

Malvern Instruments Ltd.

Regis Technologies, Inc.

With the 1290 Infinity HDR DAD the determination of low and high dose drugs present in a fixed dose combination drug was possible in one run, due to the increased linear dynamic range by >30x.

A new type of method development that uses modeling to find the "best" separation for high performance liquid chromatography (HPLC) was investigated and principles of Quality by Design (QbD) were followed when planning the investigation. The process delivered precise results and the method was able be transferred to a routine quality control (QC) laboratory.

The update to the SANCO guidelines in 2009 recognized the power and potential of TOF mass spectrometry for pesticide screening applications.

Polyimide coated fused silica capillary tubing is widely used in the separation sciences. In this application note, we share perspectives on modifying the internal surface of capillary tubing.

A commercially available monoclonal antibody was analyzed via two different particle sizes (5 ?m and 3 ?m) and column lengths (100 mm and 30 mm) to investigate their effects on resolution and the ability to increase sample throughput.

Phthalates are plasticizers that are added to plastics to make them softer and more flexible. Plastics that contain phthalates are commonly used in the manufacturing of toys. Since phthalates are not permanently bonded to plastics, they can be released from toys through touching, licking, and chewing.

Using a novel new-design monolithic silica column (Chromolith? HighResolution RP-18e), fast, high resolution separations were achieved for various drug mixtures without the high pressures characteristic of modern particulate technology.

Sample pretreatment is often a time consuming and labor-intensive step which can be a restricting factor for the rapid analysis. This is especially true for the analysis of drugs in biofluids containing many interfering matrices.

EPA Method 8270 is used to determine the concentration of semi-volatile organic compounds in extracts prepared from solid waste matrices such as soils, air sampling media, and water samples. Extractions from water samples can be particularly difficult and time consuming due to the problematic analytes that need to be extracted, concentrated, and analyzed.

Pickering Laboratories Inc.

In this article, the analysis of bath salts (phenethylamine and cathionone compounds) from urine samples is demonstrated using polymeric solid phase extraction (SPE) sample preparation followed by HILIC chromatographic analysis with TOF-MS detection. HILIC chromatographic conditions on the Ascentis? Express HILIC phase are used for fast, high-resolution separation of nine synthetic bath salts.

H2 is commonly used as GC carrier gas as the cost of He is increasing and its availability is decreasing. In addition to the cost/availability issues, H2 can provide more rapid separations at lower temperatures than He and provides longer column life. H2 gas is obtained by the electrolysis of water and many laboratories supply it to the GC via an in-house H2 generator.

Tosoh Bioscience LLC

Hyaluronic acid (HA) is a ubiquitous, very high molar mass polysaccharide that has been of particular importance in opthalmic surgery. HA acts as a molecular "shock-absorber" and stabilizer for cells and its visco-elastic properties are valuable for separating tissue and maintaining shape.

A novel method for Drug Antibody Ratio determinations based on SEC-MALS in conjunction with UV absorption and differential refractive index detection.

CUSTODION SPME Syringes provide a simple, low cost methodology to collect and introduce samples to an analytical instrument. The low cost and convenience of SPME allows multiple samplers to be deployed simultaneously.

Method 525.3 is similar in many ways to its predecessor, method 525.2 (Rev 2.0 – 1995), however there are significant changes which make the newer 525.3 a vastly improved method during sample collection, extraction, and analyzing.

LC–MS-MS has become an important tool to assess an individual's vitamin D status. Chromatographic resolution of the various homologs of vitamin D2 is necessary for accurate quantitation since several key metabolites are isobaric and not distinguishable by MS alone.

OI Analytical

A commercially available monoclonal antibody was analyzed via three different particle sizes (5 ?m, 3 ?m, and 2 ?m) to investigate the effects of particle size on resolution and the ability to increase sample throughput.

Imtakt USA