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Liposome Characterizatio by FFF–MALS–QELS
February 1st 2012Liposomes are made of lipid bilayers and are often used in drug delivery by encapsulating the core with therapeutic drugs. During liposome research, formulation, manufacturing, and quality control, it is of great importance to monitor liposome size and encapsulation.
Faster, More Sensitive Determination of Carbamates in Drinking Water
February 1st 2012U.S. EPA Method 531.2 describes the determination of widely used carbamate pesticides in raw surface water using HPLC with fluorescence detection following postcolumn derivatization, which enhances method sensitivity and selectivity as compared to UV absorbance detection.
Analysis of Over 15 Million Dalton Polymers by Aqueous SEC Method
February 1st 2012Size exclusion chromatography (SEC) is a simple HPLC technique used for the molecular weight (MW) distribution analysis of polymers. Several manufacturers provide SEC columns with different base materials and various pore sizes to cover a wide range of polymer analysis.
LC–MS-MS Method for the Determination of Enalapril and Enalaprilat fro Human Plasma Using SOLA
February 1st 2012A liquid chromatography tandem mass spectrometry method for enalapril and enalaprilat from human plasma has been developed using Thermo Scientific SOLA cartridges. Sample preparation is fast, efficient, and reproducible giving excellent recovery levels for each compound.
Microwave Assisted Extraction of Dioxins and Furans Compared to Soxhlet
February 1st 2012Microwave assisted extraction (MAE) offers significant benefits over traditional soxhlet extraction for dioxins and furans determination from environmental samples including higher sample throughput, time savings, reduced solvent and great reproducibility.
Fast Cap IC Determinations of Inorganic Anions and Cations in Drinking Water
February 1st 2012The determination of common inorganic anions and cations in drinking water is important due to the toxicity of anions (e.g., fluoride, nitrite, and nitrate) and secondary contaminants (e.g., chloride and sulfate) which can affect the water's aesthetics.
Screening for Pyrethroid Insecticides in Sediment Samples by GC–MS-MS
February 1st 2012A simple screening method for Pyrethroid insecticides in sediment samples is described. Electron impact ionization (EI) with tandem MS-MS in combination with programmed temperature vaporization injection (PTV) were used in order to obtain excellent specificity and low limits of detection.
Quantitation of Omega Fatty Acids by HPLC and Charged Aerosol Detection
February 1st 2012Omega-3 and -6 fatty acids are essential compounds required for normal growth. Omega-3 consumption is purported to have a number of health benefits, e.g., cancer prevention, cardiovascular disease prevention, and improved immune function. Although both omega-3 and -6 fatty acids can give rise to eicosanoid-signaling molecules (prostaglandins, prostacyclins, thromboxanes, and leukotrienes), the omega-6 eicosanoids are generally proinflammatory. A person's diet requires a balance of omega fatty acids at suitable levels.
Analysis of Multiple Classes of Cigarette Smoke Constituents by GCxGC-TOFMS
February 1st 2012GCxGC-TOFMS provided a comprehensive analysis of tobacco smoke. Individual smoke constituents across several target compound classes were extracted, chromatographically resolved, then identified and quantified with mass spectral detection.
UHPLC Column Protection Dramatically Extends UHPLC Column Performance and Lifetime
February 1st 2012UHPLC columns can significantly improve chromatographic separations, but they also present unique challenges. Once the UHPLC system components are optimized, perhaps your greatest concern is protecting the column from the damaging effects of microparticulates and sample contaminants.
New HPLC/UHPLC Assay Methods for Impurities in Tetracycline
February 1st 2012Tetracycline (TC), a common antibiotic used to treat urinary tract infections, acne, gonorrhea, and other conditions, yields a toxic degradation product, 4-epianhydrotetracycline (EATC). General Chapter 226 of the U.S. Pharmacopeia and National Formulary (USP-NF), referred to by monographs for epitetracycline and drug products containing tetracycline hydrochloride (TC-HCl), prescribes an antiquated assay for EATC impurity in TC.
HPLC Method for the Separation of Nine Key Components of Milk Thistle
February 1st 2012Silymarin, derived from the milk thistle plant, has been used as a natural remedy for the treatment of a number of liver diseases as well as in the protection of the liver from potential toxins. More recently, silymarin has been connected to antitumor promoting activity (1). It is therefore of interest to have available analytical methods for the analysis of silymarin components in various matrices.
Determination of Dyes in Fish Tissue by HPLC/UV
February 1st 2012Various triarylmethane and fluorescent dyes are used in aquaculture as antibacterial, antifungal, and anthelmintic agents for the treatment of fish disease. The FDA has banned the importation of certain fish from several countries due to possible contamination of these food products.