UHPLC

Trypsin is one of the most commonly used proteases in peptide mapping protocols because of its high level of specificity. However, trypsin alone is not always sufficient for full sequence coverage. In this article, the authors detail how trypsin was combined with chymotrypsin to overcome this, and the benefits of an automated platform.

Sauce-flavor or sauce-aroma baijiu, one variant of a popular Chinese liquor, is treated with a fermentation starter called daqu, giving the concoction a unique taste profile, and scientists want more information about how it can inform the manufacturing of higher-quality baijiu.

While both branched-chain fatty acids (BCFAs) and straight-chain fatty acids (SCFAs) were analyzed, the profiling of BCFAs was considered especially important for future metabolomics and lipidomics studies.

A water-resistant, highly adsorptive molecularly imprinted polymer was developed to address the issues of unstable adsorption performance and high organic solvent consumption in traditional materials.

Authentication of popular ginseng-based products has been difficult and has caused confusion in quality control between traditional Chinese medicine (TCM) and Chinese patent medicine (CPM).

OECD-compliant amino acid derivative assay (ADRA) testing and method transfer to UHPLC conditions is presented.

How to modernize an older pharmaceutical monograph from an HPLC column to an UHPLC column, along with calculated time and solvent savings.

The team of researchers found that components with the same retention time can have different migration patterns, and discussed the different scenarios for decreasing column pressure resistance.

Davy Guillarme of the University of Geneva said in a talk at HPLC 2023 that short columns have shown promise, but ultrahigh-performance liquid chromatography (UHPLC) instrumentation is not yet optimized to realize their benefits.

In his plenary lecture at HPLC 2023, Michal Holčapek presented his team's findings on how chromatography can help scientists record the human lipodome and better our understanding of diseases.

At HPLC 2023, Matthias Pursch presented his findings on using UHPLC and SEC to monitor small molecule and polymer reactions in real time.

An ultrahigh-pressure liquid chromatography–tandem mass spectrometry method (UHPLC–MS/MS) using polarity switching, preceded by solid-phase extraction (SPE), was used to quantify eight ultraviolet filter (UVF) compounds in chlorinated outdoor pools in Winnipeg.

A research team has developed a fast and sensitive method using UHPLC–MS/MS to simultaneously detect the nematicide fluensulfone (FSF) and its two major metabolites in different agricultural soils, providing valuable insights into the environmental behavior of FSF in soil systems.

Nails were evaluated for their suitability as non-invasive biomarkers for exposure to antibiotics, even those that are commonly prescribed.

A newly developed UHPLC–MS/MS method allows for the accurate analysis of bioactive compounds, including apigenin, apigenin 7-glucoside, and chlorogenic acid, in goat serum.

Why is SFC becoming increasingly more important as a sustainable analytical technique?

The evolution of biomolecule analysis is discussed in this article.

How to enhance the sensitivity, specificity, and speed of metabolite measurement using TIMS, which in turn helps to accelerate the pace of metabolomics research.

A new study has identified potential quality-markers for Eucommiae Folium (Duzhongye), a traditional Chinese medicine, using UHPLC–MS/MS analysis, and has recommended isoquercitrin as a new quality-marker candidate.

This article compares the performance of wide-pore silica monolithic, sub-2µm FPP, and SPP columns, addresses the question of whether 1000 Å or 400 Å SPP columns are more suitable for reversed-phase LC-type protein separations, and presents a kinetic performance comparison of different columns.

A micro-pillar array format for mapping the proteome of human stem cell-derived liver organoids using timsTOF–MS is presented.

This article highlights an advantage of porous graphitic carbon columns, that is their resistance at high temperatures, for the analysis of parabens in foodstuffs.

An introduction from guest editor, Gert Desmet from Vrije Universiteit Brussel, Belgium

Exploring the impact of green chemistry on analytical chemistry and GC is the focus of this instalment of "GC Connections".

A rapid and robust LC–MS/MS method for determining lactulose and rhamnose concentrations in blood plasma was used to determine intestinal permeability from blood plasma, which can help diagnose gastrointestinal diseases such as Crohn’s disease.

Everyone is talking about sustainability, and organizations are creating sustainability programs. But what does green chemistry really mean, and how does it apply to gas chromatography?

Selective pressurized liquid extraction and multilayer solid‑phase extraction methods are described for high‑throughput plant, soil, and water sample preparations. Optimal analytical conditions are described for the improvement of detection sensitivities and coverage. A Source Supported Suspect Screening (4S) approach is described; phytotoxins detected in the source plant were used to improve the identification of phytotoxins in soil and water.

Serial coupling of different column types can provide several important benefits to the chromatographer, including fine-tuning selectivity to separate complex mixtures.

In this extended special feature to celebrate the 35th anniversary edition of LCGC Europe, key opinion leaders from the separation science community explore contemporary trends in separation science and identify possible future developments.