LC–MS

Researchers at the University of Utah's (Salt Lake City) Sports Medicine Research and Testing Laboratory developed a test for testosterone and epitestosterone in urine that uses liquid chromatography with tandem mass spectrometry detection (LC-MS-MS).

Researchers at the Universit? di Camerino in Camerino, Italy have developed a new GC-MS and LC-MS technique for the determination of five ink photoinitiator residues: 2-isopropylthioxanthone (ITX), benzophenone, 2-ethylhexyl-4-dimethylaminobenzoate (EHDAB), 1-hydroxycyclohexyl-1-phenyl ketone (IRGACURE 184) and ethyl-4-dimethylaminobenzoate (EDAB) in packaged beverages.

Researchers at the Chinese Academy of Sciences and Tsingua University have developed an automated on-line HPLC-MS method allowing injection of a large volume of urine for the improvement of sensitivity using estrogens as analytes.

This month Chromatography Online's Technology Forum looks at the topic of LC-MS and the trends and issues surrounding it. Joining us for this discussion is Scott Berger of Waters Corporation, Graham McGibbon of Advanced Chemistry Development, Inc. (ACD/Labs), Bob Classon of Shimadzu Scientific Instruments, Inc., and Alexander Shaw, Ph.D of Hitachi High Technologies America, Inc.

In the last decade, research on the detection of all groups of doping agents has been investigated by LC-MS, and routine LC-MS screening applications are now available for almost all classes of doping agents.

Vitamin D is a group of fat-soluble prohormones that helps the body absorb calcium and maintain proper levels of calcium and phosphorus in the blood. A vitamin D deficiency results in impaired bone mineralization and leads to bone-softening diseases, such as rickets in children and osteomalacia and osteoporosis in adults, as well as autoimmune and nervous system diseases. A vitamin D overdose can raise blood levels of calcium and cause gastrointestinal symptoms and kidney disease. High blood levels of calcium also can cause heart rhythm abnormalities and calcinosis, the deposition of calcium and phosphate in the body's soft tissues.

This article explores the progress that atmospheric pressure photoionization (APPI) has made in its relatively short history for LC–MS analysis. Specifically, the authors examine the combination of APPI and electrospray ionization (ESI).

This article describes a LC-MS method for the analysis of biodiesels, particularly the characterization of FAMES.

This article aims to demonstrate the unique capability of resin-based mixed-mode solid-phase extraction (SPE) to achieve efficient extraction of basic drugs from plasma. Optimized pore size of the method minimizes retention of high molecular weight matrix components present in the biological fluid samples. The interferences are removed, ensuring extremely clean extracts for subsequent liquid chromatography?mass spectrometry-mass spectrometry (LC-MS-MS) analysis.

Liquid chromatography–mass spectrometry (LC–MS) is a popular technique for the analysis of wine. This article gives an overview of wine analysis and new insights this technique has revealed regarding the composition of wine, possible health benefits, customer safety and the understanding of winemaking processes.

The unique features of silica hydride-based columns are described with a focus on how these columns can expand a laboratory's capabilites in HPLC and LC-MS. Of particular interest is the ability to retain both polar and nonpolar compounds and provide an orthogonal method of analysis for currently existing procedures.

A fast quantitation method for the analysis of N-Methyl Carbamate pesticide residues in vegetables and grains has been developed. High recoveries were obtained for most carbamates screened in all matrices tested at 1 ppb levels. Good linearity of the calibration curves was observed for all analytes, over the range from 1 ppb to 200 ppb levels, with r2 greater than 0.99.

This month's column brings much of the previous discussion on how to interpret mass spectra to a practical examination of detecting substitutions in counterfeit pharmaceuticals.

LC-MS-MS has become a widely used technique for the fast and sensitive quantitation of small molecules. In this article, this approach has been extended to high-throughput quantitative LC-MS-MS analysis under GLP applications for a drug candidate in development from preclinical animal studies through clinical development.

In this month's "MS-The Practical Art," Michael Balogh takes a closer look at insight provided by Chuck McEwen regarding obstacles while using solvents.

Emerging as a complementary analytical tool to LC-MS, LC-ICP-MS brings added value to the determination of inorganic element containing molecules of biological importance.

Ultrahigh performance liquid chromatography (LC)–time-of-flight mass spectrometry –(TOF-MS) and gas chromatography (GC)–TOF-MS are powerful approaches for screening target compounds and identifying or characterizing nontarget compounds in complex mixtures. The combination of accurate mass data and newly developed software enables truly generic screening methods with TOF-MS, and the confident detection, identification, and confirmation of small molecules in a range of application areas.

Enabling targeted quantitative proteomics applications and hypothesis-driven inquiries will help researchers to understand how proteins function in living systems. The discovery and validation of small molecule and protein-based biomarkers, and the eventual translation of these discoveries from the research lab to the clinic, involves robust mass spectrometry (MS) systems and software that make it easier for technicians to perform routine sample analyses on liquid chromatography (LC)–MS-MS systems, which continue to be used in an increasing number of both protein and small molecule analysis applications.

Serum protein profiling using mass spectrometry (MS) is one of the most promising approaches for biomarker identification.

Mass spectrometry has long been a preferred tool for protein identification and biomarker discovery, but preparation of biological samples remains a challenge. Hindrances include the wide range of protein concentrations, sample complexity, and loss or alteration of important proteins due to sample handling. This article describes recent developments in sample fractionation technologies that are overcoming these challenges in interesting ways and are enabling in-depth proteomic studies that were not possible in the past.

Doping testing is now an accepted fact of sporting life - whether in major international competitions, such as the Olympics, or in regular events at the national level. Athletes who cheat by taking banned substances risk harming their careers and their health. They also bring their country and their sport into disrepute. A reliable and reproducible scientific system for doping testing, backed up by sanctions, helps fight the culture of drugs in sport so that athletes can participate on a level playing field.

September 2006. Top-down and bottom-up are alternative strategies for protein identification and characterization by mass spectrometry. How do they fit into the world of proteomics? What are their implications for separation technology? These questions are addressed in this installment of "Directions in Discovery."

In Part I of this two-part look at spectral interpretation, Michael Balogh examined the ability to see and appreciate the product of our analytical devices. In Part II, he takes a closer look at the tools of the trade.

Midazolam is a central nervous system depressant that is commonly used as a sedative before surgical or other medical procedures.

In this column, Michael Balogh proposes some practices that you can adopt to enhance your prospects of being right when converting data to knowable information and applying it to solving problems.

In this tutorial, the mechanism and origin of ion suppression will be investigated, as well as ways to validate the presence, and circumvent or compensate for, the effects in LC-MS.

This is the second installment of a two-part series on the practical aspects of configuring and operating a nano liquid chromatography-mass spectrometry (LC-MS) system.