Researchers Improve Sensitivity of Large Volume Urine Injections with HPLC-MS
Researchers at the Chinese Academy of Sciences and Tsingua University have developed an automated on-line HPLC-MS method allowing injection of a large volume of urine for the improvement of sensitivity using estrogens as analytes.
Researchers at the Chinese Academy of Sciences and Tsingua University have developed an automated on-line HPLC-MS method allowing injection of a large volume of urine for the improvement of sensitivity using estrogens as analytes. The injection volume, reporetedly, increased ten times compared with conventional on-line LC-MS methods for biofluid analysis. Moreover, no obvious increase of column pressure was observed after 300 injections. The method was validated by spiking urine. Linearity was determined by sample which was in the range of 1-500 ng/mL. The process efficiency ranged from 70.2 to 106% with RSDs less than 15% with the exception of a few analytes.
Common Challenges in Nitrosamine Analysis: An LCGC International Peer Exchange
April 15th 2025A recent roundtable discussion featuring Aloka Srinivasan of Raaha, Mayank Bhanti of the United States Pharmacopeia (USP), and Amber Burch of Purisys discussed the challenges surrounding nitrosamine analysis in pharmaceuticals.
Advances in Non-Targeted Analysis for PFAS in Environmental Matrices
March 27th 2025David Megson from Manchester Metropolitan University in Manchester, UK, spoke to LCGC International about the latest developments in non-targeted analysis (NTA) of per- and polyfluoroalkyl substances (PFAS) in environmental matrices based on a recent systematic review paper he has collaboratively published (1).
