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Advantages of Sequential or Bracketed Injection Methods to Improve the Chromatographic Analysis of BiotherapeuticsThis article explores three case studies where autosampler injection programs help to (i) reduce the carry-over observed for large nucleic acids during anion-exchange chromatography (AEX), (ii) reduce peak distortion and breakthrough for monoclonal antibodies (mAbs) during hydrophilic-interaction chromatography (HILIC), and (iii) facilitate dissolution of lipid nanoparticles (LNPs) so that a size-exclusion chromatography (SEC) method can be applied for ribonucleic acid (RNA) payload analysis.
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GCxGC with Fluidic Modulation on Enantioselective Oil AnalysisDescribes a novel approach for the enantioselective separation of spearmint oil incorporating a fluidic modulation system.
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Analysis of Bovine Serum Albumin (BSA) Digest on a Thermo Scientific Accucore 150-C18, NanoLC ColumnThermo Scientific Application Note
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Field-Flow Fractionation Coupled with ICP-MS for the Analysis of Engineered Nanoparticles in Environmental SamplesThis article provides an overview of the capabilities of field-flow fractionation coupled with inductively coupled plasma–mass spectrometry and demonstrates that the technique shows a great deal of promise to separate, detect and quantitate nanoparticles in environmental matrices.
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Toward Quantitative Imaging Mass SpectrometryThe main limitations of quantification using MALDI imaging are discussed and the different approaches used for quantitative measurement in MSI are evaluated.
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Astaxanthin from Shrimp By-products for Active Packaging SystemsThe two year international project "Preparation of active packaging with antioxidant and antimicrobial activity based on astaxanthin and chitosan",1 which started in 2009, was funded by FONCYCIT (Fund for International Cooperation of Science and Technology between the European Union and Mexico).
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Coupling Supercritical Fluid Chromatography with Evaporative Light Scattering Detection: A Study of the Relevant Parameters Ruling ResponseThe mobile-phase flow rate and the amount of modifier mixed with carbon dioxide are the main parameters affecting peak area.
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Recent Progress in Chiral Stationary Phase Development and Current Chiral ApplicationsA review of chiral separations, which remain a decided area of interest, particularly in the pharmaceutical and agrochemical fields.
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Interactive Liquid Chromatography of Olefin-based ElastomersThe use of interactive liquid chromatography using porous graphite as a stationary phase and gradients of long chain alcohols and chlorinated aromatics to separate olefin-based elastomers according to their chemical composition is discussed.
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Characterization and Quantification of Phorbol Esters by Tandem ESI-FT-ICR-MSThis article reports on the isolation, purification, and characterization of phorbol esters in Jatropha curcas seeds.
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Taming the Regulatory Beast: Regulation versus FunctionalismThe authors discuss the issue of meeting the demands of regulatory compliance whilst ensuring good scientific practice. A number of requirements from 21 CFR Part 11 are cited to demonstrate the importance of applying the principles of risk analysis.
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In-Depth Analysis of Host Cell Protein (HCP) Impurities by LC–MS/MS to Augment Routine HCP-ELISA Testing of BiotherapeuticsLC–MS/MS has gained momentum as an orthogonal approach to ELISA for host-cell protein (HCP) analysis. LC–MS/MS can identify and quantify individual HCPs, and help ensure that no HCPs evade detection above a reportable limit.
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Next-Generation Stationary Phases: Properties and Performance of Core–Shell ColumnsThis short review focuses on the physicochemical properties of core–shell particles and their resulting chromatographic efficiencies as they relate to the comparison of their mass transfer kinetics to those of columns packed with sub-2-?m fully porous particles.
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Asymmetric Flow Field Flow Fractionation Coupled with ICP-MS for Speciation Analysis of Plasma ProteinsThis work demonstrates the potential of the simultaneous on-line hyphenation of asymmetric flow field flow fractionation (AF4) to inductively coupled plasma-mass spectrometry (ICP-MS) and ultra violet-visible/multi angle light scattering (UV-vis/MALS) detection as a promising and simple tool to obtain information on the multi-element speciation and absolute molecular mass of human plasma proteins such as albumin and transferrin: the two main proteins that have been proposed to bind and transport metal ions in this biological fluid.
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Headspace Solid-Phase Microextraction Coupled with Gas Chromatography–Mass Spectrometry for the Characterization of Polymeric MaterialsHS-SPME-GC–MS is a valuable technique for identifying volatile organic compounds, additives, and degradation products in industrial rubber, car labeling reflection foil, and bone cement materials.
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Routine Multiresidue Pesticide Analysis using the Agilent 6470 Triple Quadrupole Mass SpectrometerThis application note describes a UHPLC/MS/MS-based method for the screening and quantification of more than 250 pesticides and pesticide metabolites in food samples.
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UHPLC Coupled with Accurate Mass and High Resolution Mass Spectrometry for Complex Environmental AnalysesEnvironmental analyses of food, soil, and water have changed dramatically over the last decade. Topics such as pesticides, food additives, and natural products have become important as food products are globally grown and distributed (1). Monitoring their quality is critical to international business. Pharmaceuticals, fluorinated surfactants, and endocrine disruptors in water are major new topics, where not only parent compounds are unknown but also their metabolites and degradation products are often more important or more abundant than the parent compound (2). New environmental issues, such as hydraulic fracturing and its wastewater, have captured our attention as the production of oil and gas has increased exponentially in the past decade (3). With this technology comes the problem of wastewater disposal and groundwater contamination. These environmental issues have greatly benefited from the combination of ultrahigh‑performance liquid chromatography (UHPLC) mated to high resolution mass spectrometry (HRMS
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Analysis of Pharmaceuticals and Personal Care Products in River Water Samples by UHPLC–TOF-MSA study of pharmaceuticals and personal care products in river water samples is presented from northeastern United States using ultrahigh-pressure liquid chromatography (UHPLC) coupled with TOF-MS for both targeted and nontargeted analytes
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Coupling SFC with ELSD: Study of the Parameters Ruling ResponseThe article investigates the ELSD response variation with supercritical carbon dioxide-based mobile phases and compares the response with HPLC.
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Astaxanthin from Shrimp By-products for Active Packaging SystemsThe two year international project "Preparation of active packaging with antioxidant and antimicrobial activity based on astaxanthin and chitosan",1 which started in 2009, was funded by FONCYCIT (Fund for International Cooperation of Science and Technology between the European Union and Mexico).
Latest:
Astaxanthin from Shrimp By-products for Active Packaging SystemsThe two year international project "Preparation of active packaging with antioxidant and antimicrobial activity based on astaxanthin and chitosan",1 which started in 2009, was funded by FONCYCIT (Fund for International Cooperation of Science and Technology between the European Union and Mexico).
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Elevated Temperatures in Liquid Chromatography, Part III: A Closer Look at the van 't Hoff EquationAn exploration of the usefulness and relevance of the van 't Hoff equation using various examples from the literature
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An Accurate Mass Database for the Analysis of Pesticides and Veterinary Drugs using Liquid Chromatography-High-Resolution Mass SpectrometryHere, the process to build an accurate mass database is thoroughly described and applications are commented on.
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Use of Synthetic Peptides to Determine Relative Retention Times for Automated Targeted Protein/Peptide Quantification Method DevelopmentProtein/peptide discovery data obtained from data-dependent experiments have been used to increase the success rate of targeted quantification assays as well as automate method building.
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Hollow-Fibre Liquid-Phase Microextraction in the Three-Phase Mode — Practical ConsiderationsThis month's "Sample Prepartion Perspectives" describes practical aspects of hollow-fibre liquid-phase microextraction in the three phase mode (HF3LPME) and also electromembrane extraction (EME), which uses an electrical potential as the driving force for the extraction.
