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Coupling Supercritical Fluid Chromatography with Evaporative Light Scattering Detection: A Study of the Relevant Parameters Ruling ResponseThe mobile-phase flow rate and the amount of modifier mixed with carbon dioxide are the main parameters affecting peak area.
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Recent Progress in Chiral Stationary Phase Development and Current Chiral ApplicationsA review of chiral separations, which remain a decided area of interest, particularly in the pharmaceutical and agrochemical fields.
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Interactive Liquid Chromatography of Olefin-based ElastomersThe use of interactive liquid chromatography using porous graphite as a stationary phase and gradients of long chain alcohols and chlorinated aromatics to separate olefin-based elastomers according to their chemical composition is discussed.
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Characterization and Quantification of Phorbol Esters by Tandem ESI-FT-ICR-MSThis article reports on the isolation, purification, and characterization of phorbol esters in Jatropha curcas seeds.
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Taming the Regulatory Beast: Regulation versus FunctionalismThe authors discuss the issue of meeting the demands of regulatory compliance whilst ensuring good scientific practice. A number of requirements from 21 CFR Part 11 are cited to demonstrate the importance of applying the principles of risk analysis.
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In-Depth Analysis of Host Cell Protein (HCP) Impurities by LC–MS/MS to Augment Routine HCP-ELISA Testing of BiotherapeuticsLC–MS/MS has gained momentum as an orthogonal approach to ELISA for host-cell protein (HCP) analysis. LC–MS/MS can identify and quantify individual HCPs, and help ensure that no HCPs evade detection above a reportable limit.
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Next-Generation Stationary Phases: Properties and Performance of Core–Shell ColumnsThis short review focuses on the physicochemical properties of core–shell particles and their resulting chromatographic efficiencies as they relate to the comparison of their mass transfer kinetics to those of columns packed with sub-2-?m fully porous particles.
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Asymmetric Flow Field Flow Fractionation Coupled with ICP-MS for Speciation Analysis of Plasma ProteinsThis work demonstrates the potential of the simultaneous on-line hyphenation of asymmetric flow field flow fractionation (AF4) to inductively coupled plasma-mass spectrometry (ICP-MS) and ultra violet-visible/multi angle light scattering (UV-vis/MALS) detection as a promising and simple tool to obtain information on the multi-element speciation and absolute molecular mass of human plasma proteins such as albumin and transferrin: the two main proteins that have been proposed to bind and transport metal ions in this biological fluid.
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Headspace Solid-Phase Microextraction Coupled with Gas Chromatography–Mass Spectrometry for the Characterization of Polymeric MaterialsHS-SPME-GC–MS is a valuable technique for identifying volatile organic compounds, additives, and degradation products in industrial rubber, car labeling reflection foil, and bone cement materials.
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Routine Multiresidue Pesticide Analysis using the Agilent 6470 Triple Quadrupole Mass SpectrometerThis application note describes a UHPLC/MS/MS-based method for the screening and quantification of more than 250 pesticides and pesticide metabolites in food samples.
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UHPLC Coupled with Accurate Mass and High Resolution Mass Spectrometry for Complex Environmental AnalysesEnvironmental analyses of food, soil, and water have changed dramatically over the last decade. Topics such as pesticides, food additives, and natural products have become important as food products are globally grown and distributed (1). Monitoring their quality is critical to international business. Pharmaceuticals, fluorinated surfactants, and endocrine disruptors in water are major new topics, where not only parent compounds are unknown but also their metabolites and degradation products are often more important or more abundant than the parent compound (2). New environmental issues, such as hydraulic fracturing and its wastewater, have captured our attention as the production of oil and gas has increased exponentially in the past decade (3). With this technology comes the problem of wastewater disposal and groundwater contamination. These environmental issues have greatly benefited from the combination of ultrahigh‑performance liquid chromatography (UHPLC) mated to high resolution mass spectrometry (HRMS
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Analysis of Pharmaceuticals and Personal Care Products in River Water Samples by UHPLC–TOF-MSA study of pharmaceuticals and personal care products in river water samples is presented from northeastern United States using ultrahigh-pressure liquid chromatography (UHPLC) coupled with TOF-MS for both targeted and nontargeted analytes
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Coupling SFC with ELSD: Study of the Parameters Ruling ResponseThe article investigates the ELSD response variation with supercritical carbon dioxide-based mobile phases and compares the response with HPLC.
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Astaxanthin from Shrimp By-products for Active Packaging SystemsThe two year international project "Preparation of active packaging with antioxidant and antimicrobial activity based on astaxanthin and chitosan",1 which started in 2009, was funded by FONCYCIT (Fund for International Cooperation of Science and Technology between the European Union and Mexico).
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Astaxanthin from Shrimp By-products for Active Packaging SystemsThe two year international project "Preparation of active packaging with antioxidant and antimicrobial activity based on astaxanthin and chitosan",1 which started in 2009, was funded by FONCYCIT (Fund for International Cooperation of Science and Technology between the European Union and Mexico).
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Elevated Temperatures in Liquid Chromatography, Part III: A Closer Look at the van 't Hoff EquationAn exploration of the usefulness and relevance of the van 't Hoff equation using various examples from the literature
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An Accurate Mass Database for the Analysis of Pesticides and Veterinary Drugs using Liquid Chromatography-High-Resolution Mass SpectrometryHere, the process to build an accurate mass database is thoroughly described and applications are commented on.
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Use of Synthetic Peptides to Determine Relative Retention Times for Automated Targeted Protein/Peptide Quantification Method DevelopmentProtein/peptide discovery data obtained from data-dependent experiments have been used to increase the success rate of targeted quantification assays as well as automate method building.
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Hollow-Fibre Liquid-Phase Microextraction in the Three-Phase Mode — Practical ConsiderationsThis month's "Sample Prepartion Perspectives" describes practical aspects of hollow-fibre liquid-phase microextraction in the three phase mode (HF3LPME) and also electromembrane extraction (EME), which uses an electrical potential as the driving force for the extraction.
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The Benefits of Coupling Miniaturized Comprehensive 2D LC with Hybrid High-Resolution Mass SpectrometryComprehensive two-dimensional liquid chromatography (LC×LC) is evolving and becoming more commonly used in practice, but there are some specific problems still present that hamper the widespread use of this technology. One key aspect is the coupling of an on-line LC×LC system to a mass spectrometer. Generally, on-line LC×LC is based on a very fast second dimension separation to achieve low cycle times. This often results in flow rates that are far above the optimum for electrospray ionization mass spectrometry (ESI-MS). This month’s “Multidimensional Matters” looks at the benefits of miniaturization in the first and second dimension for coupling with a high-resolution mass spectrometer (HRMS) and describes an environmental analysis application.
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Dietary Supplements Method Modernization—A Review of Current Efforts to Modernize Compendial Methods and a Summary of Allowable AdjustmentsThe implementation of the FSMA (Food Safety Modernization Act) and the continued globalization of the food supply have increased the need for improved analytical methods for quality and safety testing. Food safety and quality requirements are increasingly stringent, as are consumer expectations. In addition to new methods, the industry continues to look for improvements in laboratory productivity and new ways to reduce costs. These trends extend to dietary supplements and natural products. This article discusses the current state of efforts to modernize US Pharmacopeia (USP) compendial methods to take advantage of new chromatography technology, including the use of “allowable adjustments”.
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Continuous Chromatographic Separation of Salt From Sugars by Simulated Moving Bed Technology (SMB) Using Orochem Zucchero ColumnsSimulated Moving Bed technology (SMB) is a continuous chromatography technique widely used in the pharmaceutical/biotechnology industry. The continuous chromatographic separation of sodium sulphate (Na2 SO4) from a mixture of glucose and mannose was established by using the Orochem Zuccheroâ„¢ columns and the SMB technology. The main advantages of SMB over batch chromatography includes better yields, higher purity, and decreased solvent usage, which together make the SMB technology economically viable and ideal for desalting applications.
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Branching Out: Mass Spectrometry and the Shape of BiotherapeuticsThose fond of puns point out that mass spectrometry (MS) has become ever more focused in the last two decades, while at the same time offering ever more information. The dynamic market for biotherapeutics has driven a number of developments, particularly following the paradigm of well-characterized biopharmaceutical products (WCBP) (1,2). Partly as a result of automation and interfacing, those trained in biological or biochemical disciplines now use mass spectrometers routinely. This also means that the sorts of questions asked of MS have changed. Coping with biomolecule heterogeneity is a key challenge, not generally an issue for small molecule drugs. The data complexity means that mass information alone is insufficient. And at the submission stage, regulators are increasingly concerned about tertiary structure and conformation, something that was not previously an analytical requirement (2). Adding polyethylene glycol (PEG) to already heterogeneous molecules to prolong their half-lives in the body raises..
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A Review of Column Developments for Supercritical Fluid ChromatographyThe authors review the requirements for columns specifically designed and manufactured for SFC.
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GCxGC-FID for Qualitative and Quantitative Analysis of PerfumesExploring the use of GCxGC-FID as a technique for qualitative and quantitative analysis of perfumes
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Development of Fully Automated Bubble-in-Drop Microextraction–Gas Chromatography–Mass Spectrometry of Organochlorine Pesticides in Environmental WatersIn this study, the intentional incorporation of an air bubble into a solvent droplet is proposed for fully automated bubble-in-drop microextraction–gas chromatography–mass spectrometric (BID-GC–MS) analysis of selected organochlorine pesticides as model analytes.
