Application Notes

Natural and synthetic phenolic compounds have been used in a wide range of applications, from antiseptics and fungicides to food additives.

Pharmaceutical and personal care products (PPCPs) are products used for personal health or cosmetic reasons.

In mass spectrometry instrumentation, the sensitivity and resolution of the instrument is often limited by the detector.

Monoclonal antibodies (mAbs) form a major class of biologics and recently biosimilars and biobetters are being added to the growing inventory of therapeutics.

We have previously shown the ability of a novel stationary phase to separate 55 amino acids without derivatization (LCGC 32(3), 211 [2014]).

A purified polyclonal antibody (IgG) is separated and fully characterized using the Viscotek SEC-MALS 20, allowing calculation of molecular weight and radius of gyration (Rg).

Chiral separations of multi-component natural product mixtures comprised of enantiomers, diastereomers, and unrelated molecules are often difficult to resolve in a single isocratic method.

This application describes a solid phase extraction (SPE) method for EPA method 8141B analytes, including organophosphorus compounds, and triazine herbicides in water.

Alprostadil (Prostaglandin E1) is a drug that has vasodilation properties and is used to treat erectile dysfunction and other medical conditions.

Hydrophilic interaction liquid chromatography (HILIC) is one of the fastest growing modes of separation, in which any polar chromatographic surface can be used.

Two species of spearmint oil (mentha spicata and mentha gracilis) are cultivated in the United States.

Pain management LC analyses can be difficult to optimize due to the limited selectivity of C18 and phenyl-hexyl phases.

Hydrophilic interaction chromatography (HILIC) provides an alternative approach for the separation of highly polar substances.

A novel method for drug antibody ratio determinations based on SEC-MALS in conjunction with UV absorption and differential refractive index detection.

QuEChERS is a quick-easy-cheap-effective-rugged-safe extraction method developed in 2003 for the extraction of pesticide residues in agricultural commodities (1,2).

This application note demonstrates how comprehensive 2D-LC can be used to resolve the complex mixture of hydrophilic phenols found in virgin olive oil and investigates differences in the phenol composition of several olive oils.

Selectivity is the most powerful tool to optimize separations in HPLC. This parameter is changed by using different bonded phases, including C18, polar embedded, phenyl bonded phases and perflorophenyl, or by changing the mobile phase. In this work, 4.6 ? 50 mm Poroshell 120 columns are used to quickly evaluate method development choices for the analysis of non-steroidal anti-inflammatory drugs (NSAIDS).

A new fused-core particle designed specifically for the separation of proteins and monoclonal antibodies with 3.4 ?m particle size and a thin 0.2 ?m porous shell demonstrates high resolution of the heavy and light chain fragments of IgG1.

Shimadzu Europa GmbH

UCT, LLC

Agilent Technologies

Wyatt Technology Corporation

Bruker Daltonics

Biotage GB Ltd

Agilent Technologies

The unique surface chemistry of the Flare diamond core-shell column combines ionic and hydrophobic separation mechanisms to effectively retain a variety of chemical species in a single run.

High throughput analysis of amino acids has been a goal for both column and instrument manufacturers for many years.

Polyether antibiotics are commonly used for preventing coccidiosis and other infections in poultry and for improving feed efficiency for beef cattle and swine.

In this application we demonstrate the analysis of coffee and coffee products according to DIN methods. The performance of the analysis is shown for linearity, limit of detection (LOD), limit of quantification (LOQ), retention time, and area precision.

Gas chromatography coupled to high resolution time-of-flight mass spectrometry (GC–HRT) was used to identify halogenated organic compounds based on their mass defects in fish tissue extracts.