Application Notes

This Application Note shows the transfer of a standard HPLC method to a UHPLC method for the analysis of 13 DNPH-derivatized aldehydes and ketones. By transferring the method from HPLC to UHPLC, the analysis time and solvent consumption was reduced by approximately 90 %.

This Application Note shows the transfer of a standard HPLC method to a UHPLC method for the analysis of 13 DNPH-derivatized aldehydes and ketones. By transferring the method from HPLC to UHPLC, the analysis time and solvent consumption was reduced by approximately 90 %.

This Application Note describes UHPLC methods to simultaneously separate and identify seven tannin compounds as aging markers in barrel-aged alcoholic beverages. The proposed methodology was applied to four different matrixes: whisky, brandy, rum, and tequila, to demonstrate a correlation between aging time and total tannin concentration level.

This Application Note shows the easy transfer of an LC method from the Waters Alliance 2695 LC to an Agilent 1290 Infinity II LC, with ISET, while controlled through Waters Empower software. Paracetamol and its impurities were analyzed on both systems, and the resulting retention times and resolution values were compared.

This Application Note shows the easy transfer of an LC method from the Waters Alliance 2695 LC to an Agilent 1290 Infinity II LC, with ISET, while controlled through Waters Empower software. Paracetamol and its impurities were analyzed on both systems, and the resulting retention times and resolution values were compared.

Agilent Intelligent System Emulation technology (ISET) in the Agilent 1290 Infinity II LC facilitates the transfer of methods from conventional HPLC systems such as the Agilent 1100 Series Binary LC. This Application Note illustrates the advantages of ISET for the analysis of six tricyclic antidepressants.

Agilent Intelligent System Emulation technology (ISET) in the Agilent 1290 Infinity II LC facilitates the transfer of methods from conventional HPLC systems such as the Agilent 1100 Series Binary LC. This Application Note illustrates the advantages of ISET for the analysis of six tricyclic antidepressants.

This Application Note shows the transfer of an analysis method for anti-epileptic drugs from HPLC to UHPLC using the Agilent 1290 Infinity II LC system. An enormous time and solvent saving of approximately 95 % was achieved with an UHPLC separation optimized for speed.

The Agilent 1290 Infinity II High Dynamic Range Diode Array Detection Impurity Analyzer Solution combines the signals from two diode array detectors with different path length Max-Light cartridge cells to increase the linear dynamic UV-range. This solution can be used to detect and quantify main and trace compounds in a single run without exceeding the linear UV-range.

This Application Note shows the transfer of an analysis method for anti-epileptic drugs from HPLC to UHPLC using the Agilent 1290 Infinity II LC system. An enormous time and solvent saving of approximately 95 % was achieved with an UHPLC separation optimized for speed.

Ultrahigh throughput is shown for ultrafast gradients with over 60 % reduction of total analysis time using alternating column regeneration. This was accomplished with parallel injection by the second needle installed in the Agilent 1290 Infinity II Multisampler, which has a dual-needle option for the analysis of food preservatives.

Ultrahigh throughput is shown for ultrafast gradients with over 60 % reduction of total analysis time using alternating column regeneration. This was accomplished with parallel injection by the second needle installed in the Agilent 1290 Infinity II Multisampler, which has a dual-needle option for the analysis of food preservatives.

The Agilent 1290 Infinity II High Dynamic Range Diode Array Detection Impurity Analyzer Solution combines the signals from two diode array detectors with different path length Max-Light cartridge cells to increase the linear dynamic UV-range. This solution can be used to detect and quantify main and trace compounds in a single run without exceeding the linear UV-range.

"This Application Note shows the analysis of 20 explosives with the Agilent 1290 Infinity II Variable Wavelength Detector (VWD) in dual-wavelength mode using 254 and 214 nm."

This Application Note describes the use of the Agilent 1290 Infinity II LC for the automated operation of seven different applications using different methods in food analysis. The exchange of columns is controlled by the method used. All columns are located in a single Agilent 1290 Infinity II Multicolumn Thermostat. Accordingly, the Agilent 1290 Infinity II Flexible Pump is attached to two external solvent-selection valves for automated solvent switching.

Evaporative light scattering (ELS) is a unique and highly sensitive technique for detection of semivolatile and nonvolatile solutes in a liquid stream. As a consequence of the physics of light scattering, conventional ELS detectors have a relatively small dynamic range. In contrast, the Agilent 1290 Infinity II Evaporative Light Scattering Detector has a 10-times higher dynamic range compared to conventional ELS detectors.

This Application Note describes the use of the Agilent 1290 Infinity II LC for the automated operation of seven different applications using different methods in food analysis. The exchange of columns is controlled by the method used. All columns are located in a single Agilent 1290 Infinity II Multicolumn Thermostat. Accordingly, the Agilent 1290 Infinity II Flexible Pump is attached to two external solvent-selection valves for automated solvent switching.

Evaporative light scattering (ELS) is a unique and highly sensitive technique for detection of semivolatile and nonvolatile solutes in a liquid stream. As a consequence of the physics of light scattering, conventional ELS detectors have a relatively small dynamic range. In contrast, the Agilent 1290 Infinity II Evaporative Light Scattering Detector has a 10-times higher dynamic range compared to conventional ELS detectors.

"This Application Note shows the analysis of 20 explosives with the Agilent 1290 Infinity II Variable Wavelength Detector (VWD) in dual-wavelength mode using 254 and 214 nm."

Comprehensive 2D-LC enables the analysis and quantification of purine alkaloids and catechins in green and black tea. As expected, green tea contained higher amounts of the catechins epigallocatechin gallate and epicatechin than black tea.

This automated SPE methodology is a solution that will reduce analyst labor, solvent usage, and turn-around-time while maintaining the high quality results required within today's laboratories.

TSKgel UP-SW3000 columns are 2 µm SEC columns designed for the analysis of monoclonal antibodies and other biopharma products. Higher resolution can be achieved for the separation of antibody monomers, dimers, and higher order aggregates with a TSKgel UP-SW3000 column compared to a competitor UHPLC column. The TSKgel UP-SW3000 column provided excellent reproducibility for the peak parameters of retention time, asymmetry, and column efficiency. As demonstrated by the %RSD values, injection-to-injection reproducibility was superior to the competitor column.

Homogenize 2 g of dry tea leaves with 25 mL of Li220 for 5 min. Centrifuge and filter through 0.45 µm nylon filter.

TSKgel UP-SW3000 columns are 2 µm SEC columns designed for the analysis of monoclonal antibodies and other biopharma products. Higher resolution can be achieved for the separation of antibody monomers, dimers, and higher order aggregates with a TSKgel UP-SW3000 column compared to a competitor UHPLC column. The TSKgel UP-SW3000 column provided excellent reproducibility for the peak parameters of retention time, asymmetry, and column efficiency. As demonstrated by the %RSD values, injection-to-injection reproducibility was superior to the competitor column.

Following sampling, tubes were transferred to a Markes UNITY™ thermal desorption system paired with an Agilent 7890B GC that was coupled to an Agilent 5977A MS detector. The UNITY™ system and GC–MS parameters are presented in Table I and Table II, respectively.

The US EPA has implemented an exposure research program aimed at conducting drinking water research on methods as part of the Microbiological and Chemical Exposure Assessment. This research is aimed at evaluating the chemical pollutants and their role and levels in which there is an unacceptable risk to either humans or wildlife, as well as evaluating the methodologies currently being used to determine levels of chemical pollutants.