Application Notes

This application note describes the GC¬¬–MS analysis of 23 drugs of abuse from various compound classes such as amphetamines, benzodiazepines, cannabinoids, and other illicit drugs.

A simple sample preparation procedure and a rapid, sensitive, specific UHPLC–MS-MS method has been developed for quantifying several antidepressants and metabolites.

Using a single LC–MS-MS application, 12 steroids were separated. However, three separate panels are also run as an alternative method.

A simple sample preparation procedure and a rapid, sensitive, specific UHPLC–MS-MS method has been developed for quantifying several antidepressants and metabolites.

This application note describes the GC¬¬–MS analysis of 23 drugs of abuse from various compound classes such as amphetamines, benzodiazepines, cannabinoids, and other illicit drugs.

The collection of oral fluid is gaining popularity due to the fact that it is not intrusive and easy to use. In this study we look at four different devices, and compare the results obtained for drugs varying in terms of hydrophobicity and their acidity/basicity.

The collection of oral fluid is gaining popularity due to the fact that it is not intrusive and easy to use. In this study we look at four different devices, and compare the results obtained for drugs varying in terms of hydrophobicity and their acidity/basicity.

This podcast will focus on new developments involving our understanding of disinfection by products in drinking water. We will cover updates to toxicological compounds and advancements in new technologies to identify them.

Data integrity problems in pharmaceutical quality control laboratories are driving more regulatory action than ever before. It is obvious that something has changed to drive all this activity. There is plenty of information available, but much of it seems to confuse or frustrate rather than clarify or help. In this summary, we will provide clarity, dispelling confusion by looking at the facts, based on a study of available resources and direct interactions with FDA staff and their consultants. You’ll learn from Loren Smith, Agilent’s software compliance expert and a UC Berkeley instructor with 25 years of regulated software experience, how to put the current enforcement environment in historical context, and to apply critical thinking skills to what you hear or read regarding data integrity. You’ll also learn how to evaluate your current laboratory software and associated processes against these new expectations, as well as how vendors are redesigning laboratory software to help you respond to these new realities.

Explore the potential for screening human blood fortified with ethylenediaminetetraacetic acid (EDTA) via LC/MS using an expression single quadrupole compact mass spectrometer.

Recently the US EPA published a list of 30 UCMR4 (the 4th Unregulated Contaminant Monitoring Rule) analytes which may potentially be present in tap water but are not yet subject to EPA’s drinking water standards set under the Safety Drinking Water Act. Four among the 30 UCMR4 compounds, including o-toluidine, quinoline, butylated hydroxyanisole (BHA), and dimethipin, are determined by EPA method 530 using solid phase extraction (SPE) and GC/MS detection. In this study, analytes were extracted using UCT’s divinylbenzene based sorbent (HLDVB). GC/MS SIM method with solvent standard calibration was carried out for data acquisition and analyte quantitation.

The increased use of hormone-based therapies in health care throughout the world has resulted in hormones finding their way into municipal water supplies. The potential health risks of unintended consumption of hormones through drinking water have significantly increased the interest in identifying these compounds in our water supplies. The Milli-Q system incorporates a combination of purification processes that provides ultrapure, hormone-free lab water for the LC–MS techniques used for interference-free analysis of these contaminants.

Historically, UV detection has been favored in many laboratories for its ease of use, robustness, and reliability. However, some of the inherent challenges include analytes that do not have a response in a UV channel, coelutions, and unknowns, any of which can require an orthogonal approach such as mass detection. While mass detection offers a number of benefits when used in tandem with UV detection, incorporating it into an existing UV workflow can be time-consuming and laborious.

Data integrity problems in pharmaceutical quality control laboratories are driving more regulatory action than ever before. It is obvious that something has changed to drive all this activity. There is plenty of information available, but much of it seems to confuse or frustrate rather than clarify or help. In this summary, we will provide clarity, dispelling confusion by looking at the facts, based on a study of available resources and direct interactions with FDA staff and their consultants. You’ll learn from Loren Smith, Agilent’s software compliance expert and a UC Berkeley instructor with 25 years of regulated software experience, how to put the current enforcement environment in historical context, and to apply critical thinking skills to what you hear or read regarding data integrity. You’ll also learn how to evaluate your current laboratory software and associated processes against these new expectations, as well as how vendors are redesigning laboratory software to help you respond to these new realities.

Ion chromatography (IC) is a well-established technique for monitoring inorganic anions in environmental waters around the world. It is approved by the U.S. Environmental Protection Agency (U.S. EPA) for compliance monitoring of primary and secondary inorganic anions in drinking water and has been validated by European standards organizations, including the International Standards Organization (ISO). These agencies have published IC methods for the analysis of inorganic anions in drinking water, groundwater, and wastewater. These methods can be made faster and more cost-effective, however, by using the latest IC technologies, including high-pressure IC. In this summary, we will discuss regulatory compliance testing of municipal drinking water and wastewater, and how this testing can be made faster and more cost-efficient through the use of high-pressure-capable ion chromatography systems.

Using a targeted solid phase extraction (SPE) method and GC–ECD analysis, chlorinated pesticides are extracted from poultry fat resulting in a method that decreases the amount of labor and reagents when compared to the USDA-FSIS CHC2 method.

A simple, automatable cleanup method is developed for the analysis of acrylamide from coffee, yielding absolute recoveries that exceed 92% when analyzed by LC–MS-MS.

Using a targeted solid phase extraction (SPE) method and GC–ECD analysis, chlorinated pesticides are extracted from poultry fat resulting in a method that decreases the amount of labor and reagents when compared to the USDA-FSIS CHC2 method.

A simple, automatable cleanup method is developed for the analysis of acrylamide from coffee, yielding absolute recoveries that exceed 92% when analyzed by LC–MS-MS.

Cannabinoids are successfully extracted from a complex brownie matrix using the QuEChERS sample preparation technique, significantly reducing the presence of matrix interferences. The resulting clean samples are analyzed by GC–MS using a specialized deactivated GC column to provide heightened peaks for the cannabinoid compounds.

EPA Method 625 is greatly simplified by using solid phase extraction (SPE) as an alternative to traditional liquid-liquid extraction (LLE). Using this SPE technique, paired with a sensitive GC–MS analysis, increases throughput and data quality, while decreasing manual labor and solvent usage.

Cannabinoids are successfully extracted from a complex brownie matrix using the QuEChERS sample preparation technique, significantly reducing the presence of matrix interferences. The resulting clean samples are analyzed by GC–MS using a specialized deactivated GC column to provide heightened peaks for the cannabinoid compounds.

Improving and optimizing EPA Method 593 using solid phase extraction (SPE) enhances sensitivity and maximizes efficiency while screening by LC–MS-MS for different female birth control hormones present in drinking and waste water.

EPA Method 625 is greatly simplified by using solid phase extraction (SPE) as an alternative to traditional liquid-liquid extraction (LLE). Using this SPE technique, paired with a sensitive GC–MS analysis, increases throughput and data quality, while decreasing manual labor and solvent usage.

Phospholipid removal proves to yield superior results as compared to a traditional protein precipitation step when analyzing a complex plasma matrix via LC–MS-MS. The results displayed a decrease in ion suppression, increased analyte sensitivity, and an improvement in column lifetime.

Enhancing the extraction of vitamins A and E in serum using simplified liquid extraction (SLE) significantly improves the LC–MS-MS detection of target compounds, all while preserving a simple and robust method.

Phospholipid removal proves to yield superior results as compared to a traditional protein precipitation step when analyzing a complex plasma matrix via LC–MS-MS. The results displayed a decrease in ion suppression, increased analyte sensitivity, and an improvement in column lifetime.

Enhancing the extraction of vitamins A and E in serum using simplified liquid extraction (SLE) significantly improves the LC–MS-MS detection of target compounds, all while preserving a simple and robust method.

Using oral fluid as the matrix, a viable and simple solid phase extraction method for a wide range of drugs is developed. Several oral fluid collection devices were evaluated to determine the effectiveness of the cleanup procedure, ultimately confirming that the method is both robust and widely applicable.

Improving and optimizing EPA Method 593 using solid phase extraction (SPE) enhances sensitivity and maximizes efficiency while screening by LC–MS-MS for different female birth control hormones present in drinking and waste water.