Application Notes

Using oral fluid as the matrix, a viable and simple solid phase extraction method for a wide range of drugs is developed. Several oral fluid collection devices were evaluated to determine the effectiveness of the cleanup procedure, ultimately confirming that the method is both robust and widely applicable.

An ultra-sensitive sample preparation technique using solid phase extraction (SPE) microelution 96-well plates effectively concentrates peptides and allows for cleanup of small volume.

A suite of unknown drug compounds from whole blood are analyzed by LC–MS-MS, requiring the need for an effective yet nonspecific cleanup technique. The resulting method demonstrates a simple and fast sample preparation procedure that is suitable for screening many compounds.

A suite of unknown drug compounds from whole blood are analyzed by LC–MS-MS, requiring the need for an effective yet nonspecific cleanup technique. The resulting method demonstrates a simple and fast sample preparation procedure that is suitable for screening many compounds.

An ultra-sensitive sample preparation technique using solid phase extraction (SPE) microelution 96-well plates effectively concentrates peptides and allows for cleanup of small volume.

The objective of this application note is to demonstrate that the ACQUITY QDa Detector provides a simple and cost-effective solution for detecting oligonucleotides across a wide molecular weight range and can be readily integrated into existing UV-based workflows.

This summary will explore the latest developments in the analysis of contaminants of emerging concern in drinking water. We will explore the requirements and results from the EPA’s Unregulated Contaminant Monitoring Rule 3 program for Perfluorinated organics, and hormones. After a brief overview of the EPA analytical techniques, we’ll take a look at new technology and ask how it compares for both and if it can find compounds we didn’t even know were there.

Intact and reduced IgGs have been successfully retained and separated using a non-porous column. Potential applications of Presto columns for a single-injection separation of free drug, antibody, and antibody-drug complexes (ADCs) is also discussed.

The validation process of an analytical method is a complex and demanding activity, consisting of many time-consuming steps. Empower® 3 Method Validation Manager (MVM) automates the method validation workflow within a single software environment, reducing time and ensuring conformance to the validation requirements and acceptance criteria defined in the protocol. This application note demonstrates validation of a UPLC® method for metoclopramide HCl and related substances using Empower 3 MVM.

Hamilton polymeric PRP-X100 and PRP-X110 anion exchange columns are the easy way to separate inorganic and organic anions from 10 ppb to 500 ppm without elaborative preconcentration or suppression.

Uncertainty in chromatographic method development and quantitative experiments often arises. RADAR can detect uncertainty, discover co-eluting compounds, collect full scan MS data and monitor the background matrix, enabling fast method development.

This Application Note demonstrates the separation and quantification of beta-blockers, listed as prohibited substances by the World Anti-Doping Agency, in urine using the Agilent 1260 Infinity Analytical SFC System.

HPLC column stability is a critical factor. This note describes how the Agilent Poroshell HPH-C18 column performed brilliantly in an elevated pH mobile phases such as ammonium bicarbonate buffer.

This Application Note demonstrates the separation and quantification of beta-blockers, listed as prohibited substances by the World Anti-Doping Agency, in urine using the Agilent 1260 Infinity Analytical SFC System.

Ion Chromatography Applications and Detection Methods from the Thermo Scientific portfolio of products for the detection of inorganic anions and cations, toxic contaminants, and disinfection byproducts.

Determination and quantification of water contamination by phenols using the Dionex AutoTrace 280 SPE instrument.

A method incorporating direct analysis in real time (DART) ionization and Thermo Scientific™ Orbitrap™ high-resolution mass spectrometry for rapid analysis and identification of contaminating substances in water.

Specifications and data highlighting the Thermo Scientific™ Dionex™ IonPac™ CS16-4μm and CS16-Fast-4μm cation-exchange columns for the determination of disparate concentration ratios of sodium and ammonium in diverse sample matrices.

Automated Solid-phase extraction (SPE) with GC-ECD for the determination of 15 nitrobenzene compounds in finished drinking water using the automated Thermo Scientific™ Dionex™ AutoTrace 280 Solid Phase-Extraction instrument in conjunction with the Thermo Scientific™ TRACE™ 1310 gas chromatograph.

Development of an ion chromatography (IC) method using suppressed conductivity detection for the determination of Cr(VI) in drinking water.

A method for the determination of pyrethroids in water at ultra-low-level concentrations of 0.02 and 0.10 ng/mL was developed using solid-phase extraction (SPE) for pre-concentration and subsequent analysis by GC with PTV injection.

Analysis of OCPs, PAHs and PCBs in environmental samples through a consolidated GC-MS/MS method using the Thermo Scientific™ TRACE™ 1310 GC and the TSQ™ 8000 triple quadrupole GC-MS/MS.

WAX type columns are the industry standard analyzing a wide range of compounds with polar functional groups. The new Agilent DB-WAX Ultra Inert GC column is presented.

WAX type columns are the industry standard analyzing a wide range of compounds with polar functional groups. The new Agilent DB-WAX Ultra Inert GC column is presented.