Chiral Separation of Beta Blocker Pindolol Enantiomers
Pindolol is a non-selective beta-blocker used for treatment of hypertension and angina pectoris. It is applied as a racemate, although only the (S)-form is the active stereoisomer.
YMC offers immobilized chiral phases that can be used either in normal or reversed phase mode as well as in supercritical fluid chromatography (SFC) mode. CHIRAL ART Cellulose-SC is a very versatile phase to separate many chiral substances. YMC have developed two isocratic applications to separate the pindolol enantiomers in normal phase or reversed phase mode. Both methods offer a high resolution. The normal phase application provides more potential for a preparative scale up, while the reversed phase approach separates the enantiomers in a shorter time.
Normal Phase Method
Reversed Phase Method
Figure 1: Structure of pindolol.
Figure 2: Normal phase method.
Figure 3: Reversed phase method.
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SEC-MALS of Antibody Therapeutics—A Robust Method for In-Depth Sample Characterization
June 1st 2022Monoclonal antibodies (mAbs) are effective therapeutics for cancers, auto-immune diseases, viral infections, and other diseases. Recent developments in antibody therapeutics aim to add more specific binding regions (bi- and multi-specificity) to increase their effectiveness and/or to downsize the molecule to the specific binding regions (for example, scFv or Fab fragment) to achieve better penetration of the tissue. As the molecule gets more complex, the possible high and low molecular weight (H/LMW) impurities become more complex, too. In order to accurately analyze the various species, more advanced detection than ultraviolet (UV) is required to characterize a mAb sample.
