Application Notes

This Application Note describes the comparison of complex hop aroma profiles by GC–MS, and the identification of aroma-active terpenoids aided by soft electron ionisation.

This Application Note describes the comparison of complex hop aroma profiles by GC–MS, and the identification of aroma-active terpenoids aided by soft electron ionisation.

High pressures and columns with small particle sizes have been used in high-performance liquid chromatography (HPLC) for a long time, but not in ion chromatography (IC). But now, high pressures are coming to IC. Read this new e-book to learn about: • The advantages of high-pressure and small-particle-size columns in IC • How those advantages apply to real-world examples in the analysis of food, beverages, and environmental samples • The reasons why the move to high-pressures in IC has taken so long • The drivers for the trend toward higher-capacity IC columns . . . and more! Download the e-book today!

Studies of adenosine monophosphate (AMP) and phosphorylated sugar in rat blood have become increasingly important in toxicology and insulin investigation. HPLC is typically the ideal method for this study. Shodex VT-50 2D would be the ultimate column due to its cAMP and AMP capabilities.

Initial precision was demonstrated by spiking four 1-L volumes with one Snip and Pour pre-measured standard, each (40 mg). The data for four replicates was collected for 47 mm Disks and 100 mm Disks. The average percent recovery is excellent and meets the criterion specified of 83–101% HEM recovery for both size disks. The standard deviation is better than the criterion specified of 11% for HEM.

A variety of alcoholic beverages purchased within the UK were analyzed for the presence of Ethylcarbamate. A 1 µL injection of these alcoholic beverages were analyzed on an Ellutia 200 GC followed by detection on an Ellutia 820 TEA working in nitrogen detection mode. This mode of analysis detects nitrogen containing compounds within a sample. The Ellutia 810/820 systems are able to analyse various nitro or nitroso compounds, utilizing the selectivity available through nitrogen or nitroso modes. Nitrogen mode utilizes the catalytic pyrolyzer tube, oxygen reactor, and higher temperatures to detect nitrogen compounds. Results for various alcoholic beverages are shown in Figure 3. The samples were found to have various low levels of ethylcarbamate, the system was optimized through levels of oxygen input and EHT sensitivity. The samples were then analyzed adopting a standard addition approach.

Hamilton PRP-X100 and PRP-X110 are the perfect solution for anion analysis in water or environmental samples. The robust design and covalent surface modification of the packing material lead to a highly increased lifetime as compared to the competition. The production process is compliant to ISO 9001 ensuring an outstanding column-to-column and lot-to-lot reproducibility.

This application note describes the development of an analytical method for the sensitive and accurate determination of free and total thyroid hormones in serum and plasma using a mixed-mode SPE extraction procedure and UHPLC–MS-MS analysis.

The standards are commercially available from Wellington Laboratories Inc. (Ontario, Canada): TF-TCDD-MXB.

Analysis of the six matrices processed yielded acceptable recoveries for all analytes with standard deviations below 20%. Analysis of an n-hexane blank sample resulted in no detectable target analytes measured within the calibration range of each respective compound.

Commission Regulation (EC) No 152-2009 published in the official Journal of European Union laid down the methods of sampling and analysis for the official control of feed. The Regulation describes methods of analysis to control the composition of feed materials and compound feed products. Establishing the amino acids profile is an important way to control quality and nutritional value of feeds.

The ex vivo lability of some drug compounds and molecules in bio-analytical assays can add complexity and uncertainty to results and can pose significant challenges in drug development efforts. This can be especially true for compounds that are subjected to long-term cryogenic storage and that may need to be tested multiple times (for example, samples collected for clinical trials). Frozen aliquotting technology may offer a simple solution for stabilizing target compounds in frozen biological specimens, helping to streamline bio-analytical assay development and execution. The CXT 750 Frozen Sample Aliquotter, an automated instrument capable of generating quantitative aliquots of frozen plasma without thawing the sample, was evaluated at GlakoSmithKline for integration into biaonalytical workflows and the stabilization of labile compounds in EDTA plasma.

In addition to the weight-average molar mass and the polydispersity, the DAWN can also determine the shape of the polymer by measuring the rms radius directly at each elution volume. It is well-known that butadiene forms highly branched polymers and that styrene forms linear polymers-and this is revealed by the DAWN.

In the example shown here we seek to provide an alternative to HILIC methods by using a novel multi-mode column called the Scherzo SS-C18. This stationary phase has both reversed phase (C18) and polar components with the addition of a high density of strong anion and cation exchange ligands. This strategy for increasing polar retention is much more predictable run to run and does not require lengthy column pre-treatments.

Shiseido succeeded in development of CAPCELL CORE ADME S2.7, a novel stationary phase with an adamantyl group having a cage structure. This is characterized as alkyl group; therefore, the packed column is still easy to use just as a reversed-phase column.

The peak resolution profile remained nearly unchanged. In particular, the resolution profile of thyroglobulin was similar to the resolution that was obtained by the 4.6 mm ID × 30 cm column. This suggests that the separation of high order molecular weight species, such as aggregates from mAb, can be easily achieved using this 4.6 mm ID × 15 cm column. Ten consecutive runs yielded excellent reproducibility. In fact, peaks from 10 consecutive injections were nicely overlaid.

This application note describes techniques to increase sample throughput without compromising the accuracy of analysis during the monitoring of aggregate formation during biotherapeutic development

This application note describes techniques to increase sample throughput without compromising the accuracy of analysis during the monitoring of aggregate formation during biotherapeutic development

Size exclusion chromatography (SEC) is widely used for detecting aggregates, fragments, and other impurities in a biotherapeutic sample. When coupled with multi-angle light scattering (MALS), SEC enables researchers to quantify the true solution molar mass of each species in a sample, in addition to the mass fraction of each spe-cies.

Multi-angle light scattering (MALS) is primarily used to determine molecular weight, size-and shape-related parameters of macromolecules or nanoparticles as they elute from a separation device. In batch (unfractionated) mode, MALS provides the weight-average molecular weight wand the z-average mean square radius z. The DAWNHELEOSIIis particularly well suited for these tasks owing to its flow cell design, holding a mere ~67µl and readily connecting toa fluid line through HPLC-type fittings.

The dioxin family consists of 210 compounds, of which 17 contain the 2,3,7,8 pattern of chlorination. These 2,3,7,8 containing compounds are of extreme human health concern due to their high level of toxicity. Approximately, 12 of the 209 polychlorinated biphenyls have also been identified as human toxins. For this reason, the US FDA and EU have established strict regulations for the monitoring of food products for human consumption, in particular edible oils. Manual extractions of oils can be a time consuming procedure often delaying lab turnaround times. By automating the process with the EconoPrep®, food oil samples can be reliably processed with routine 24 hr turnaround times.

The dioxin family consists of 210 compounds, of which 17 contain the 2,3,7,8 pattern of chlorination. These 2,3,7,8 containing compounds are of extreme human health concern due to their high level of toxicity. Approximately, 12 of the 209 polychlorinated biphenyls have also been identified as human toxins. For this reason, the US FDA and EU have established strict regulations for the monitoring of food products for human consumption, in particular edible oils. Manual extractions of oils can be a time consuming procedure often delaying lab turnaround times. By automating the process with the EconoPrep®, food oil samples can be reliably processed with routine 24 hr turnaround times.

A fully tested LC-MS/MS workflow for rapid and robust quantification of more than 250 pesticides below maximum residue limits (MRLs) with sensitivity, accuracy, and precision that meets stringent EU guidelines.

Previously, SFE and SFC were offline operations for pretreatment or analysis, respectively, and treated as completely separate workflows. This note, however, presents a flow diagram showing the integration of SFE-SFC processes from pretreatment to data acquisition in a single workflow, as well as an analysis of reduced coenzyme Q10.

This note describes the use of TSKgel UP-SW3000, 2µm SEC columns for the analysis of proteins, with data demonstrating the operation of these columns using a simple and well established method for use in both HPLC and UHPLC systems. TSKgel UP-SW3000 columns have superior resolution for proteins and the shorter column dimension, 4.6 mm ID × 15 cm, allows runs to be completed 2 times faster than its longer column dimension counterpart without compromising resolution and reproducibility.

The use of ion-exchange sorbents for the preconcentration, separation and determination of metal ions is well established in the literature. Selection of an appropriate sorbent ensures both high efficiency in metal chelating while minimizing the mass of sorbent required for a particular analytical task. A high efficiency sorbent means that a smaller bed mass may be used thereby reducing the quantity of solvent required for elution yielding greater analytical sensitivity.

This Application Note demonstrates the sensitive and rapid determination of Polycyclic Aromatic Hydrocarbons (PAHs) in tap water using the Agilent 1200 Infinity Series Online SPE Solution.

This Application Note demonstrates the sensitive and rapid determination of Polycyclic Aromatic Hydrocarbons (PAHs) in tap water using the Agilent 1200 Infinity Series Online SPE Solution.

Centrifugal Partition Chromatography (CPC) also known as Counter Current Chromatography (CCC) is a preparative, pilot and industrial liquid purification technique that does not require traditional solid supports. CPC was used to purify few mg of gingerol from crude extract.

This Application Note describes UHPLC methods to simultaneously separate and identify seven tannin compounds as aging markers in barrel-aged alcoholic beverages. The proposed methodology was applied to four different matrixes: whisky, brandy, rum, and tequila, to demonstrate a correlation between aging time and total tannin concentration level.