Automated MAb Workflow: From Harvest Cell Culture to Intact Mass Analysis of Variants
Recombinant monoclonal antibodies (MAbs) are a growing class of therapeutic molecules. Early in the development of MAbs, a large number of harvest cell culture (HCC) samples must be screened for immunoglobulin G (IgG) titer, aggregations, and charge variants. This study describes a two-dimensional (2D) high performance liquid chromatography (HPLC) workflow for afinity, size-exclusion, and ion-exchange analysis.
Recombinant monoclonal antibodies (MAbs) are a growing class oftherapeutic molecules. Early in the development of MAbs, a large numberof harvest cell culture (HCC) samples must be screened for immunoglobulinG (IgG) titer, aggregations, and charge variants. This study describes atwo-dimensional (2D) high performance liquid chromatography (HPLC)work ow for af nity, size-exclusion, and ion-exchange analysis.
Inside the Laboratory: The Zydney Group at Pennsylvania State University
November 26th 2024In this edition of “Inside the Laboratory,” Andrew Zydney of Pennsylvania State University discusses his laboratory’s work with using high performance liquid chromatography (HPLC) to analyze biopharmaceutical products before and after membrane separation processes.
Green Chemistry: What is it (and What Is It Not)? And How Does It Apply to Gas Chromatography?
October 31st 2024Everyone is talking about sustainability, and organizations are creating sustainability programs. But what does green chemistry really mean, and how does it apply to gas chromatography?
