HPLC

Noteworthy new HPLC systems and related products

If scaling isocratic separations is so simple, why is gradient scaling so confusing?

The mechanisms of mixed-mode chromatography are explained, with reference to the parameters that are used to optimize selectivity and retention.

This article focuses on the progression that chiral stationary phases (CSPs), specifically developed for HPLC, are currently undergoing because of the pressing need of an easy switch to UHPLC.

For proper column installation it is important to be familiar with column characteristics such as solvent compatibility, maximum flow rate, and maximum operating temperature.

What kind of adjustments need to be made when scaling an isocratic method?

A simple and fast ion-pair chromatography method to detect sodium alkyl sulphates in environmental samples using conductivity detection is described.

Joe Pesek answers questions about IEX, HILIC, polar-embedded, and polar-endcapped columns, and aqueous normal phase chromatography.

What kind of adjustments need to be made when scaling an isocratic method?

Click here to view the complete E-Separation Solutions newsletter from January 17, 2014.

To ensure an efficient, reliable, and sensitive determination of all corkiness-related off-flavour compounds in wine samples, the DLR Mosel in Germany has successfully turned to GC–MS combined with stir-bar sorptive extraction.

This article provides examples of the robustness of monolithic HPLC columns and how they can be used to help overcome the inherent challenges presented when analytes are in complex matrices.

What good is that big, ugly peak at the beginning of the chromatogram?

Secondary parameters in the interface and mass analyzer can often have a major impact on sensitivity and reproducibility. This column considers how and when to consider optimizing these parameters through a study of the working principles of LC–MS analysis.

Oxycodone and its metabolites were analyzed by high performance liquid chromatography–mass spectrometry (HPLC–MS) using a diisobutyl stable bond column. This article discusses the results.

Why do some peaks front when others don't - in the same method?

A fully automated solution was developed for the determination of THC and its metabolites THC–OH and THC-COOH in serum.

How to evaluate a calibration curve and calculate the lower limit of calibration.

A new chromatography-based method for analyzing a traditional Chinese medicine and its metabolites.

An interview with Hans-Gerd Janssen of Unilever Research and Development Vlaardingen and the University of Amsterdam. Janssen is involved with method development for gas chromatography, liquid chromatography, and mass spectrometry; the development of instrumentation for multidimensional chromatography for food analysis and biomacromolecular characterization; and problem solving and efficient routine analysis of foods (particularly oils and fats). This interview discusses the challenges encountered in the analysis of fats and oils, how multidimensional chromatography can be applied to the analysis, 2D LC for food-related compound analysis, the analysis of natural antioxidants in edible oils, and future steps in his research.

Click here to view the complete E-Separation Solutions newsletter from October 17, 2013.

Click here to view the complete E-Separation Solutions newsletter from October 3, 2013.

A look at liquid chromatography in the past, what it has become today, and where it is going in the future

A number of popular myths or half-truths in UHPLC are described and data are provided that contradict some of these commonly held beliefs.

Recent advances in sampling techniques in the pharmaceutical industry sparked significant interest in applying improvements to extraction methods for greater analyte detection and quantitation.

Click here to view the complete E-Separation Solutions newsletter from September 24, 2013.

Click here to view the complete E-Separation Solutions newsletter from September 12, 2013.

This article examines the problem of dermorphin doping in horse racing and presents an effective method for its detection using LC–MS TQ.

This article reviews some of the main developments that have led to recent advances in liquid chromatography–mass spectrometry (LC–MS).

An excerpt from LCGC's e-learning tutorial on HPLC methods at CHROMacademy.com