HPLC

A look at liquid chromatography in the past, what it has become today, and where it is going in the future

A number of popular myths or half-truths in UHPLC are described and data are provided that contradict some of these commonly held beliefs.

Recent advances in sampling techniques in the pharmaceutical industry sparked significant interest in applying improvements to extraction methods for greater analyte detection and quantitation.

Click here to view the complete E-Separation Solutions newsletter from September 24, 2013.

Click here to view the complete E-Separation Solutions newsletter from September 12, 2013.

This article examines the problem of dermorphin doping in horse racing and presents an effective method for its detection using LC–MS TQ.

This article reviews some of the main developments that have led to recent advances in liquid chromatography–mass spectrometry (LC–MS).

An excerpt from LCGC's e-learning tutorial on HPLC methods at CHROMacademy.com

This troubleshooting study of a problem of retention time variation provides a good example of how to track down the source of a problem and how to speculate on probable cause.

When transferring HPLC methods between instruments, many parameters need to be considered. Knowledge of underlying principles and a few key equations will help you.

A review of the advances and trends in technology and applications discussed at the HPLC 2013 conference, including a summary of the awards presented.

In new research, separation scientists from the University of Lyon, France, have compared different configurations of on-line two-dimensional liquid chromatography (2D LC) for the separation of charged compounds such as peptides.

An automated liquid chromatography (LC) method with on-line enrichment was successfully developed for the analysis of eight chlorinated phenoxyacid herbicides in water.

Recently, LCGC asked a panel of experts (listed in the sidebar) to assess the current state of the art of liquid chromatography (LC) columns and methods and to try to predict how the technology will develop in the future.

Liquid Chromatography

Where do all those extra peaks come from?

"HPLC Particle Pioneer" Joseph Jack Kirkland answers questions from Gert Desmet on his pioneering career in chromatography and his interest in superfi cially porous particles (SPPs).

A set of extended chiral separation screens using HPLC and SFC has been tailored to meet the specific and changing needs of customers in a pharmaceutical development setting.

This article describes possible sources of error that may contribute to significant inaccuracy in peptide assay methods: The fi ltration material (cellulose) used in sample clean up and the vial material (glass and plastic) used in LC analysis. This study is based on ?-endorphin but has relevance for most peptide assays.

This tutorial on reversed-phase LC explains the role of solvent, chain conformation, solute position, and retention dynamics.

A summary of the results from a survey of 14 leading HPLC–UHPLC column experts is presented, covering the state of sub-2-?m porous particles, superficially porous (core–shell) particles, silica monoliths, and polymeric monoliths.

HPLC method development can be accelerated by using three distinct method templates of increasing complexity.

The functional principles of AF4 and HF5 are discussed.

A global lipid method has been developed using a charged aerosol detector that resolves and detects numerous classes of lipids in a single analysis. The method was used to characterize complex oil samples.

The use of UHPLC has triggered a remarkable increase in efficiency and throughput of LC and LC –MS methods. The fastest growing market segment in the pharmaceutical industry is biopharmaceuticals, with an increased interest in applying UHPLC to biopharmaceutical analysis.

Achieving a high quality sample separation is critical to the success of UHPLC analyses. Numerous practical factors must be considered to achieve this.

A new method to optimize liquid chromatography (LC) methods using a Quality by Design (QbD) approach is presented. This method is based on the use of design of experiments (DOE) and independent component analysis (ICA) to accurately estimate the modeled responses (that is, the retention times at the beginning, the apex, and the end) of each peak, even for coeluted peaks. This method was applied to the optimization of the separation of nine compounds in a mixture, yielding the design space and the demonstration of robustness of the method.

The underlying principles of gradient elution HPLC are very different from those of the isocratic mode. Appreciating the differences can lead to better methods.

The underlying principles of gradient elution HPLC are very different from those of the isocratic mode. Appreciating the differences can lead to better methods.

Evidence to demystify HPLC and UHPLC column myths