October 29th 2024
Manitoba Centre for Proteomics and Systems Biology scientists produced a new means of predicting peptide retention times for hydrophilic interaction liquid chromatography (HILIC) at acidic pH in formic-acid based eluents.
Researchers Compare Configurations for Two-Dimensional LC for the Analysis of Peptides
August 21st 2013In new research, separation scientists from the University of Lyon, France, have compared different configurations of on-line two-dimensional liquid chromatography (2D LC) for the separation of charged compounds such as peptides.
Inaccuracies in the Quantification of Peptides — A Case Study Using β-Endorphin Assay
August 1st 2013This article describes possible sources of error that may contribute to significant inaccuracy in peptide assay methods: The fi ltration material (cellulose) used in sample clean up and the vial material (glass and plastic) used in LC analysis. This study is based on ?-endorphin but has relevance for most peptide assays.
The Current Status and Future of LC Column Technology: What the Experts Are Saying
August 1st 2013A summary of the results from a survey of 14 leading HPLC–UHPLC column experts is presented, covering the state of sub-2-?m porous particles, superficially porous (core–shell) particles, silica monoliths, and polymeric monoliths.
How UHPLC and Large Molecules Fit Together
July 5th 2013The use of UHPLC has triggered a remarkable increase in efficiency and throughput of LC and LC –MS methods. The fastest growing market segment in the pharmaceutical industry is biopharmaceuticals, with an increased interest in applying UHPLC to biopharmaceutical analysis.
A New Method for Quality by Design Robust Optimization in Liquid Chromatography
A new method to optimize liquid chromatography (LC) methods using a Quality by Design (QbD) approach is presented. This method is based on the use of design of experiments (DOE) and independent component analysis (ICA) to accurately estimate the modeled responses (that is, the retention times at the beginning, the apex, and the end) of each peak, even for coeluted peaks. This method was applied to the optimization of the separation of nine compounds in a mixture, yielding the design space and the demonstration of robustness of the method.
Material Identification by HPLC with Charged Aerosol Detection
July 1st 2013A method is described that incorporates a mobile-phase gradient consistent with a HILIC separation mechanism and uses a mixed-mode column, which provides reversed-phase and cation-and anion-exchange properties, and charged aerosol detection.