Author


Colin Masui

Latest:

High-Throughput Experimentation: Where Does Mass Spectrometry Fit?

In the pharmaceutical industry, the use of mass spectrometry in high-throughput experimentation (HTE) has increased, thanks to the technique’s speed, sensitivity, and selectivity. We systematically evaluate the applicability of multiple MS techniques for different types of HTE samples and purposes, reviewing the pros and cons, and provide practical recommendations, Illustrated by application case studies.


Yifan Huang

Latest:

A Brief Review of Recent Advances in Isomeric N- and O-Glycomics

Glycan isomer expressions have not been well studied, due to inefficient separation and structural identification techniques. Fortunately, with the development of novel separation techniques and liquid chromatography–mass spectrometry (LC–MS) based glycan isomer identification strategies, new efforts have been made to investigate the glycan isomers in various diseases. Here, we review the recent advances of several isomeric separation techniques for both N- and O-linked glycans.


Wenjing Peng

Latest:

A Brief Review of Recent Advances in Isomeric N- and O-Glycomics

Glycan isomer expressions have not been well studied, due to inefficient separation and structural identification techniques. Fortunately, with the development of novel separation techniques and liquid chromatography–mass spectrometry (LC–MS) based glycan isomer identification strategies, new efforts have been made to investigate the glycan isomers in various diseases. Here, we review the recent advances of several isomeric separation techniques for both N- and O-linked glycans.


Yehia Mechref

Latest:

A Brief Review of Recent Advances in Isomeric N- and O-Glycomics

Glycan isomer expressions have not been well studied, due to inefficient separation and structural identification techniques. Fortunately, with the development of novel separation techniques and liquid chromatography–mass spectrometry (LC–MS) based glycan isomer identification strategies, new efforts have been made to investigate the glycan isomers in various diseases. Here, we review the recent advances of several isomeric separation techniques for both N- and O-linked glycans.



Liping Zhou

Latest:

Determination of α-Amanitin in Human Serum by Solid-Phase Extracton Coupled with HPLC-UV

A method is presented for the determination of ?-amanitin, a toxic cyclic octapeptide of amanitin mushrooms, in human serum by solid-phase extraction coupled with high performance liquid chromatography with UV detection.


John Lough

Latest:

Meeting Review: Emerging Separation Technologies 2019

Organized by The Chromatographic Society and the Separation Science Group of the Royal Society of Chemistry’s (RSC) analytical division, the Emerging Separation Technologies meeting was held on the 28 March 2019 at the RSC’s central London headquarters in Burlington House.


Igor Veryovkin

Latest:

Mass Spectrometry of Organic Molecules and Laser-Induced Acoustic Desorption: Applications, Mechanisms, and Perspectives

This study illustrates the versatility of a laser-induced acoustic desorption (LIAD) approach for volatilization of analytes in modern mass spectrometry.



ForteBio

Latest:

Accelerating antibody discovery by monitoring titer and glycosylation with the Octet platform

Significantly reduce time to develop antibodies by combining titer and glycan screening using Bio-Layer Interferometry. Save on FTE costs and perform more projects.


Hillel K. Brandes

Latest:

High-Throughput and High-Efficiency Separations of Antibodies by Reversed-Phase Chromatography Using Organic Alcohols

The strategies described here can improve the efficiency and analyte recovery of chromatographic methods used to characterize monoclonal antibodies and antibody– drug conjugates.


Jonathan G. Shackman

Latest:

The LCGC Blog: Remember the First Time - Chromatography

What’s the fastest way to acquire a new chromatographic skill? The answer lies with those who are just beginning to learn.


Nazim Mekaoui

Latest:

Solvent Selection in Countercurrent Chromatography Using Small-Volume Hydrostatic Columns

How to use small columns to test potential biphasic liquid systems for use in large-scale countercurrent chromatography separations




Halina Katsialevich

Latest:

Employing Mechanistic Model’s Flexibility to Create Robust UHPLC Method Design: Case Study on Industrial Cannabis sativa Sample

With the era of new complex drug products there is an increasing demand on multivariate modelling tools to integrate robust analytical method design, facilitate efficient and science-based change management, and improve communication between regulators and industry. Mechanistic models provide several advantages in terms of modelling efficiency, versatility, and high predictive power. The following case study will demonstrate a robust method design for industrial Cannabis sativa.



Maryam Lashgari

Latest:

Frontiers of Sampling: Design of High Surface Area Thin-Film Samplers for On-site Environmental Analysis

In on-site environmental applications, representative sampling and proper replication are essential. For these reasons, recent work in thin-film solidphase microextraction has focused on the development of unique holders and customized samplers that are tailored for distinct sampling environments. Here, we explore the latest developments.


Jonathan Grandy

Latest:

Frontiers of Sampling: Design of High Surface Area Thin-Film Samplers for On-site Environmental Analysis

In on-site environmental applications, representative sampling and proper replication are essential. For these reasons, recent work in thin-film solidphase microextraction has focused on the development of unique holders and customized samplers that are tailored for distinct sampling environments. Here, we explore the latest developments.


Varoon Singh

Latest:

Frontiers of Sampling: Design of High Surface Area Thin-Film Samplers for On-site Environmental Analysis

In on-site environmental applications, representative sampling and proper replication are essential. For these reasons, recent work in thin-film solidphase microextraction has focused on the development of unique holders and customized samplers that are tailored for distinct sampling environments. Here, we explore the latest developments.


Joseph Helble

Latest:

Fast LC for Conventional HPLC Systems

Many laboratory budgets do not allow the purchase of new ultrahigh-pressure liquid chromatography (UHPLC) systems, and workloads typically are not declining. Fast LC incorporates the use of faster mobile phase flow rates and smaller particles to achieve separations in less time and with equivalent resolution to traditional high performance liquid chromatography (HPLC).


Torsten Kretschmer

Latest:

Macherey-Nagel - Determination of Triphenylmethane Dyes from Aquaculture Samples

This application note describes the determination of the triphenylmethane dyes Malachite Green and Crystal Violet and their metabolites from the aquaculture samples brown trout, shrimp, and tuna using dispersive SPE (dSPE) with CHROMABOND QuEChERS mixes for sample clean-up.


Kathirvel Alagesan

Latest:

Effective Enrichment of Glycopeptides in Drop‑HILIC Approach Using iSPE®-HILIC Material

Demonstratation of the glycopeptide enrichment efficiency of iSPE®-HILIC material for glycoproteomics applications using a well characterized glycoprotein standard.


YMC America, Inc.

Latest:

Exploring the Versatile Functionality of YMC’s Contichrom® CUBE

YMC America’s J Preston and Jeff Kakaley discuss the 5-in-1 functionality of YMC’s flagship chromatography system – the Contichrom® CUBE. They’ll explore using the instrument for different modes of chromatography, the added benefits of YMC resins, and how the CUBE can be used to scale up to YMC’s TWIN MCSGP systems.


Jenõ Fekete

Latest:

HPLC Determination of Residual Primary Amine Content in Epoxy-Amine Adducts

LC Determination of Residual Primary Amine Content in Epoxy-Amine Adducts


Stefan Moser

Latest:

Design of Experiment Strategy for an Electrospray Ionization Optimization in Supercritical Fluid Chromatography–Mass Spectrometry Coupling

The optimization of adjustable factors in electrospray ionization (ESI) by design of experiments (DoE) is presented. This approach allows a comprehensive and systematic optimization of all relevant factors for the ionization of compounds and increases the understanding about factor influences on the ionization efficiency.


Hans-Werner Bilke

Latest:

Design of Experiment Strategy for an Electrospray Ionization Optimization in Supercritical Fluid Chromatography–Mass Spectrometry Coupling

The optimization of adjustable factors in electrospray ionization (ESI) by design of experiments (DoE) is presented. This approach allows a comprehensive and systematic optimization of all relevant factors for the ionization of compounds and increases the understanding about factor influences on the ionization efficiency.


Aran Paulus

Latest:

Host Cell Protein Monitoring During Downstream Processing Using Micro-Pillar Array Columns Combined with Mass Spectrometry

This article investigates host cell protein analysis using micro-pillar array columns combined with mass spectrometry.


Andrew Williamson

Latest:

Host Cell Protein Monitoring During Downstream Processing Using Micro-Pillar Array Columns Combined with Mass Spectrometry

This article investigates host cell protein analysis using micro-pillar array columns combined with mass spectrometry.


Geert Van Raemdonck

Latest:

Host Cell Protein Monitoring During Downstream Processing Using Micro-Pillar Array Columns Combined with Mass Spectrometry

This article investigates host cell protein analysis using micro-pillar array columns combined with mass spectrometry.