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Multidimensional Separation Techniques for Characterization of BiotherapeuticsMultidimensional separations, in which two or more separation methods are coupled, are a valuable analytical tool for higher peak capacity and improved selectivity for the analysis of complex samples like biotherapeutics.
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Evaluation of Achiral Stationary Phases for Gradient Screening with Supercritical Fluid ChromatographyUnlike reversed-phase liquid chromatography, SFC lacks a universal stationary phase. Thus, it is important to re-evaluate the default column screening library used with SFC. In this study, three uncommon achiral SFC columns were investigated and compared to three popular stationary phases.
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Kinase Fragments Dimerize Without Oligomerization Domains, Shown by SEC-MALSetermination of oligomeric states is an important issue in protein chemistry. For example, self-assembly via oligomerization domains is crucial for the regulation of several protein kinases. Determination of the oligomeric state of fragments of these kinases is a means of verifying the involvement of each domain in self-assembly.
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An Uncommon Fix for LC–MS Ion SuppressionMetal-free columns should be considered when troubleshooting ion suppression. Chelating compounds that can form metal salt with stainless steel column housings are examined.
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A New HILIC Column for Saccharide AnalysisDue to the highly polar nature of saccharides, the analysis of sugars is typically achieved using hydrophilic interaction chromatography (HILIC).
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SFC–GCxGC to Analyse Matrices from Petroleum and CoalSFC–GCxGC is shown to be useful in fossil fuel analysis. Practical examples for the analysis of complex matrices are described.
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Strategies for the Quantification of Endogenously Present Small Molecules in Biological SamplesThe main objective of this review article is to provide a clear summary of the different methods that can be used to quantify endogenous small molecules. Because of the increased use of mass spectrometry (MS) in the field of bioanalysis, a special focus will be placed on quantification by liquid chromatography (LC)–MS. Practical recommendations to face this bioanalytical challenge, in particular in terms of method validation, will also be provided.
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Strategies for the Quantification of Endogenously Present Small Molecules in Biological SamplesThe main objective of this review article is to provide a clear summary of the different methods that can be used to quantify endogenous small molecules. Because of the increased use of mass spectrometry (MS) in the field of bioanalysis, a special focus will be placed on quantification by liquid chromatography (LC)–MS. Practical recommendations to face this bioanalytical challenge, in particular in terms of method validation, will also be provided.
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Mobile Phase Buffers in LC: Effect of Buffer Preparation Method on Retention RepeatabilityFor liquid chromatography (LC) methods where the buffer pH and composition have an influence on retention, which buffer preparation method will provide the most repeatable results?
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Sample Treatment in Routine Analysis: A Case Study: Sulphonamides in LiverCurrently, sample treatment is still the bottleneck in the development of analytical methods to analyze complex samples, especially for routine analysis where high-tech instruments are not always available. Research on the evaluation of different sample treatments is needed to achieve the sensitivity and selectivity required. This article presents a case study related to the determination of sulphonamides in liver. Different extraction strategies, including solid-liquid extraction (SLE), ultrasound-assisted extraction (UAE), and solid-phase extraction (SPE), were evaluated to produce a simple and effective extraction method.
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Sample Treatment in Routine Analysis: A Case Study: Sulphonamides in LiverCurrently, sample treatment is still the bottleneck in the development of analytical methods to analyze complex samples, especially for routine analysis where high-tech instruments are not always available. Research on the evaluation of different sample treatments is needed to achieve the sensitivity and selectivity required. This article presents a case study related to the determination of sulphonamides in liver. Different extraction strategies, including solid-liquid extraction (SLE), ultrasound-assisted extraction (UAE), and solid-phase extraction (SPE), were evaluated to produce a simple and effective extraction method.
Latest:
Sample Treatment in Routine Analysis: A Case Study: Sulphonamides in LiverCurrently, sample treatment is still the bottleneck in the development of analytical methods to analyze complex samples, especially for routine analysis where high-tech instruments are not always available. Research on the evaluation of different sample treatments is needed to achieve the sensitivity and selectivity required. This article presents a case study related to the determination of sulphonamides in liver. Different extraction strategies, including solid-liquid extraction (SLE), ultrasound-assisted extraction (UAE), and solid-phase extraction (SPE), were evaluated to produce a simple and effective extraction method.
Latest:
Sample Treatment in Routine Analysis: A Case Study: Sulphonamides in LiverCurrently, sample treatment is still the bottleneck in the development of analytical methods to analyze complex samples, especially for routine analysis where high-tech instruments are not always available. Research on the evaluation of different sample treatments is needed to achieve the sensitivity and selectivity required. This article presents a case study related to the determination of sulphonamides in liver. Different extraction strategies, including solid-liquid extraction (SLE), ultrasound-assisted extraction (UAE), and solid-phase extraction (SPE), were evaluated to produce a simple and effective extraction method.
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A Whole Lot of Data Going On Book Review: Data Integrity and Data Governance by R.D. McDowallR.D. McDowall has written an excellent book on data integrity and data governance. Those who need to understand what this is should read the book and follow his advice. He has included both what the regulations and regulators say and what we need to do to be in compliance. This is a scalable approach with enhancements for larger items and shortcuts for smaller items, with numerous examples throughout the book.
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Analysis of Active Cannabis Compounds in Edible Food Products: Gummy Bears and BrowniesPotency testing in marijuana-infused edibles is a problematic task due to the complexity of the matrices. The concentration of active ingredients in edibles can range from a few ppm to 3.5% (1). In this application, active cannabinoid compounds were extracted from gummy bears (and also brownies, results not shown), followed by HPLC analysis.
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Optimizing the Use of High Purity Water in Ion Chromatography – Direct-Feed and Hints and TipsAs instrumentation and analytical methods are becoming increasingly sensitive, the ability to perform trace and ultra trace analyses relies on the high quality and purity of the reagents used.
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Determination of Psilocin and Psilocybin in Magic Mushrooms Using iHILIC®-Fusion and MSHallucinogenic mushrooms, known as magic mushrooms, contain psychoactive compounds such as psilocin and psilocybin (Figure 1). This hallucinogenic effect means they are constantly offered on the black market. Therefore, the reliable quantification of these compounds is a particularly important task for forensic analysis because their results have a significant impact on the judgement passed by the courts.
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Determination of Psilocin and Psilocybin in Magic Mushrooms Using iHILIC®-Fusion and MSHallucinogenic mushrooms, known as magic mushrooms, contain psychoactive compounds such as psilocin and psilocybin (Figure 1). This hallucinogenic effect means they are constantly offered on the black market. Therefore, the reliable quantification of these compounds is a particularly important task for forensic analysis because their results have a significant impact on the judgement passed by the courts.
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Determination of Psilocin and Psilocybin in Magic Mushrooms Using iHILIC®-Fusion and MSHallucinogenic mushrooms, known as magic mushrooms, contain psychoactive compounds such as psilocin and psilocybin (Figure 1). This hallucinogenic effect means they are constantly offered on the black market. Therefore, the reliable quantification of these compounds is a particularly important task for forensic analysis because their results have a significant impact on the judgement passed by the courts.
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The 30th International Symposium on Pharmaceutical and Biomedical Analysis (PBA 2019)A preview of the upcoming 30th International Symposium on Pharmaceutical and Biomedical Analysis (PBA 2019), which is due to be held on 15–18 September 2019 at the Convention Centre of The Dan Panorama Hotel in Tel Aviv, Israel.
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Gulf Coast Conference Continues to Transform the Analytical WorldThe Gulf Coast Conference (GCC) is held in Galveston annually. For the past 50+ years, this gathering of international scientists has blazed a trail for analytical needs and innovations for the refining and petrochemical industries.
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Multi-Attribute Analysis of Monoclonal AntibodiesMulti-attribute analysis combines protein A affinity chromatography, SEC, and LC/MS in a 3D setup for assessment of features including mAb titer and size variants.
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Direct Analysis of Drugs in BiofluidsSample pretreatment is often a time consuming and labor-intensive step which can be a restricting factor for the rapid analysis. This is especially true for the analysis of drugs in biofluids containing many interfering matrices.
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A Convenient Alternative to MALDI and ESIIonization of small, large, volatile, and nonvolatile compounds with charge states nearly identical to electrospray ionization are produced from a solid matrix or solution with high sensitivity utilizing the vacuum inherent with any mass spectrometer. With the proper matrix, analytes can be analyzed from ambient conditions or by direct introduction into vacuum.
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The Determination of Trace Per- and Polyfluoroalkyl Substances and Their Precursors Migrated into Food Simulants from Food Contact Materials by Liquid Chromatography–MS/MS and Gas Chromatography–MS/MSAn increasing range of per- and polyfluoroalkyl substances (PFAS) has been found migrating from food contact material into food. This article establishes an integrated analytical approach combining HPLC–MS/MS and GC–MS/MS to detect 36 such PFAS.
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The Determination of Trace Per- and Polyfluoroalkyl Substances and Their Precursors Migrated into Food Simulants from Food Contact Materials by Liquid Chromatography–MS/MS and Gas Chromatography–MS/MSAn increasing range of per- and polyfluoroalkyl substances (PFAS) has been found migrating from food contact material into food. This article establishes an integrated analytical approach combining HPLC–MS/MS and GC–MS/MS to detect 36 such PFAS.
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The Determination of Trace Per- and Polyfluoroalkyl Substances and Their Precursors Migrated into Food Simulants from Food Contact Materials by Liquid Chromatography–MS/MS and Gas Chromatography–MS/MSAn increasing range of per- and polyfluoroalkyl substances (PFAS) has been found migrating from food contact material into food. This article establishes an integrated analytical approach combining HPLC–MS/MS and GC–MS/MS to detect 36 such PFAS.
Latest:
The Determination of Trace Per- and Polyfluoroalkyl Substances and Their Precursors Migrated into Food Simulants from Food Contact Materials by Liquid Chromatography–MS/MS and Gas Chromatography–MS/MSAn increasing range of per- and polyfluoroalkyl substances (PFAS) has been found migrating from food contact material into food. This article establishes an integrated analytical approach combining HPLC–MS/MS and GC–MS/MS to detect 36 such PFAS.
