Application Notes

The separation of closely related compounds is of interest to many disciplines including natural products, flavors and fragrances, and pharmaceuticals.

The determination of enantiomeric purity is crucial in new drug development.

New efficient, effective, and sensitive sample preparation application for the extraction of ethyl glucuronide from human urine at significantly low levels using EVOLUTE AX; a resin-based mixed-mode sorbent with an optimised combination of nonpolar (hydrophobic), polar (hydrophilic), and strong anion exchange interactions.

Aflatoxins occur naturally in peanuts, cottonseed, corn, and dried chili pepper, as well as many mixed or processed foods and feeds.

This article describes a straightforward ion chromatographic method that uses isocratic elution and pulsed amperometric detection (PAD) to sensitively determine water-soluble polyols and sugar alcohols as well as mono-, di- and oligosaccharides in essential and nonessential foodstuffs.

In food, organic acids originate from the natural biochemistry processes or are added as preservatives, acidulants and stabilizers.

Using a bonded polymeric zwitterionic stationary phase, in HILIC mode, polar quaternary herbicides like Diquat can easily be retained, with good peak symmetry.

Naringenin, a chiral flavonoid found in several fruit juices, is well known for its beneficial health-related properties, including antioxidant, antimicrobial, anti-inflammatory, and immunomodulating activities. The HPLC and supercritical fluid chromatography (SFC) enantioselective separation of (R,S)-Naringenin has been performed on both analytical and semi-preparative scale using an amylose derived RegisPack CSP.

Isolation and purification of compounds and their impurities/degradants is an important part of the drug discovery and development process.

Polysaccharides are often separated using SEC columns. However, SEC columns do have limitations, specifically: poor column efficiency or peak shape.

GC–MS analysis of urine for drugs of abuse is commonplace, but can be complicated by high matrix effects and frequent co-elution.

In the recently published 2nd edition of Modern Size Exclusion Liquid Chromatography: Practice of Gel Permeation and Gel Filtration Chromatography (1) it is stated that the debate about which column to place first when running a series of SEC columns is still open.

There are currently several hormones and hormonelike agents with marked ability to improve the rate of growth and efficiency of feed intake for animals.

Tips and Tricks: GPC/SEC Copolymer Analysis in GPC/SEC 3: Two-dimensional Chromatography

Cationic surfactants are used as disinfectants, corrosion inhibitors, phase-transfer catalysts, wetting agents, dispersants, and even mobile phase modifiers in liquid chromatography.

ISOLUTE EPH columns have been developed to fractionate pentane or hexane based soil extracts into aliphatic and aromatic hydrocarbon fractions (C8?C40 aliphatics, C10?C22 aromatics).

Viruses are packets of infectious nucleic acid (either DNA or RNA) surrounded by a protective coat consisting of a large number of protein subunits.

Cocaine is a central nervous system stimulant derived from Erythroxylon coca.

Protein-based pharmaceutical drugs, such as fusion proteins and monoclonal antibodies (mAb), are playing an increasing role in modern therapeutics.

Polysaccharide-based chiral stationary phases (CSPs) are often utilized for a broad range of enantiomeric chromatographic separations due to their wide chiral recognition ability.

Furan is naturally occurring at low levels in many foods and drinks (1).

The new MAbPacTM SCX-10 column is based on 10 µm nonporous, highly cross-linked styrene-type polymeric media with a proprietary uniform hydrophilic coating that is grafted with sulfonic acid functional groups.

Chemical modification of therapeutic proteins in order to enhance their biological activity is of increasing interest.

The Chirosil RCA(+) and SCA(–) chiral stationary phases (CSPs) are specialized chiral phases very effective in separating various natural and unnatural amino acids as well as compounds containing primary amines.

An extension of ASTM D7169 is shown using new Zebron ZB-1XT SimDist metal GC columns.

In developing the Atomx automated sample prep system, Teledyne Tekmar integrated both a P&T concentrator and a multi-matrix autosampler into a single platform system that can be used in the analysis of soil, water, and soils requiring methanol extraction.

Illicit cocaine, amphetamine, methamphetamine, and ecstasy (MDMA) are often encountered in the form of salts and identification of the specific counter ion present may provide valuable information about the source of production and the manufacturing pathway.

Hydroxyethylstarches (HES) are used increasingly as plasma expanders in medical applications.

Coffees have aroma and taste based on where the beans are grown, roasting techniques, and flavor additives.

This note examines the benefits of using flash or HPLC for compound purification. Results show that flash is a viable alternative to HPLC.