The Importance of Ultrapure Water for Nano LC-MS Applications
This work presents a proof of concept that using freshly produced ultrapure water to prepare nano LC eluent does not cause emitter clogging.
Nano-flow LC (nano LC) has emerged as an important tool in proteomics research as well as in quantitative measurement of low level biomarkers. A common problem facing users of this technique is emitter clogging. To avoid this, it is important not only to use robust nanobore columns and emitters but also to make sure that the solvents and reagents are of the highest purity. This work presents a proof of concept that using freshly produced ultrapure water to prepare nano LC eluent does not cause emitter clogging. This is demonstrated by comparing the mass chromatograms of peptides over numerous injections using the same emitter and using fresh ultrapure water in the eluent, as well as by physical examination of the used emitter under a light microscope.
RAFA 2024 Highlights: Contemporary Food Contamination Analysis Using Chromatography
November 18th 2024A series of lectures focusing on emerging analytical techniques used to analyse food contamination took place on Wednesday 6 November 2024 at RAFA 2024 in Prague, Czech Republic. The session included new approaches for analysing per- and polyfluoroalkyl substances (PFAS), polychlorinated alkanes (PCAS), Mineral Oil Hydrocarbons (MOH), and short- and medium-chain chlorinated paraffins (SCCPs and MCCPs).
Advancing Bladder Cancer Research with Mass Spectrometry: A FeMS Interview with Marta Relvas-Santos
November 12th 2024LCGC International interviewed FeMS Empowerment Award winner Marta Relvas-Santos on her use of mass spectrometry to identify potential biomarkers and therapies for bladder cancer. She also shared insights on her work with FeMS and advice for fellow scientists.
