There are many factors that influence the performance of a high performance liquid chromatography (HPLC) stationary phase, of which the chemical nature of the bonded phase ligand is important, but by no means all encompassing. Minor manufacturing parameters such as the method of electropolishing the internal surface of the column can also have an effect on the selectivity and efficiency produced by a particular column.
There are many factors that influence the performance of a high performance liquid chromatography (HPLC) stationary phase, of which the chemical nature of the bonded phase ligand is important, but by no means all encompassing. Minor manufacturing parameters such as the method of electropolishing the internal surface of the column can also have an effect on the selectivity and efficiency produced by a particular column.
Few of us have time to study each individual parameter (of which there are hundreds) and assess their interactive effects on the selectivity of our stationary phases. We need readily accessible measures of column performance to identify similar or orthogonal chemistries to those we are currently using, or to gain an insight into which column types might work for particular applications.
Several attempts have been made to produce a "definitive" set of chemical probes to best characterize the huge number of stationary phases available (well over 1000 different types are currently available). As yet a harmonized set of test probes and methodologies has not been identified, however three, independent, publicly available databases of HPLC columns exist today:
Figure 1: Schematic representations of the five interactions described by the hydrophobic subtraction model (adapted from reference 5).
The PQRI database is the best populated, with 588 columns, and is a very useful tool to aid HPLC column selection. A description of the Tanaka test probes is given below to help understand the various classifications, with the analogous PQRI test probes indicated in parentheses. It should be noted that the PQRI classification uses different chemical probes to the Tanaka (now ACD) database but the results describe a similar chemical behavior.
Most of these groups have used chemometric approaches to produce quantitative comparisons between column characteristics based on principal component analysis (PCA) or tools to visualize the relative groupings of commercially available columns according to their key descriptors.
References
(1) K. Kimata, K. Iwaguchi, S. Onishi, K. Jinno, R. Eksteen, K. Hosoya, M. Arki, and N. Tanaka, J. Chromatogr. Sci. 27, 721 (1989).
(2) M.R. Euerby and P. Petersson, J. Chromatogr. A 994, 13–36 (2003).
(3) M.R. Euerby and P. Petersson, J. Chromatogr. A 1088, 1–15 (2005).
(4) M.R. Euerby and P. Petersson, J. Chromatogr. A 1154, 138–151 (2007).
(5) N.S. Wilson, M.D. Nelson, J.W. Dolan, L.R. Snyder, R.G. Wolcott, and P.W. Carr, J. Chromatogr. A 961, 171–193 (2002).
(6) J.W. Dolan, A. Maule, D. Bingley, L. Wrisley, C.C. Chand, M. Angod, C. Lunte, R. Krisko, J.M. Winston, B.A. Homeier, D.V. McCalley, and L.R. Snyder, J. Chromatogr. A 1057, 59–74 (2004).
(7) L.R. Snyder, A. Maule, A. Heebsh, R. Cuellar, S. Paulson, J. Carrano L. Wrisley, C.C. Chan, N. Pearson, J.W. Dolan, and J.J. Gilroy, J. Chromatogr. A 1057, 49–57 (2004).
(8) N.S. Wilson, M.D. Nelson, J.W. Dolan, L.R. Snyder, R.G. Wolcott, and P.W. Carr, J. Chromatogr. A 961, 195–215 (2002).
GC–TOF-MS Finds 250 Volatile Compounds in E-Cigarette Liquids
November 1st 2024A study has used gas chromatography coupled to a time-of-flight mass spectrometer to build an electron ionization mass spectra database of more than 250 chemicals classified as either volatile or semi-volatile compounds. An additional, confirmatory layer of liquid chromatography–mass spectrometry analysis was subsequently performed.
Green Chemistry: What is it (and What Is It Not)? And How Does It Apply to Gas Chromatography?
October 31st 2024Everyone is talking about sustainability, and organizations are creating sustainability programs. But what does green chemistry really mean, and how does it apply to gas chromatography?
HILIC Peptide Retention Times Predicted Using New Approach
October 29th 2024Manitoba Centre for Proteomics and Systems Biology scientists produced a new means of predicting peptide retention times for hydrophilic interaction liquid chromatography (HILIC) at acidic pH in formic-acid based eluents.