Liquid Chromatography (LC/HPLC)

There are many factors that influence the performance of a high performance liquid chromatography (HPLC) stationary phase, of which the chemical nature of the bonded phase ligand is important, but by no means all encompassing. Minor manufacturing parameters such as the method of electropolishing the internal surface of the column can also have an effect on the selectivity and efficiency produced by a particular column.

Incognito found a 2D LC solution to a tricky separation problem but this technique did not meet the client's requirements. The magnanimous columnist admits to a rare defeat on this occasion but praises the thrill of seeking a solution to a perplexing method development problem. Could any readers suggest a more straightforward solution?

In honor of LCGC's celebration of 30 years covering the latest developments in separation science, we asked a panel of experts (listed in the sidebar) to assess the current state of the art of liquid chromatography (LC) column technology, and to try to predict how the technology will develop in the future. This article is part of a special group of articles covering the state of the art in sample preparation, gas chromatography (GC) columns, GC instrumentation, LC columns, and LC instrumentation.

Automated column and mobile phase screening is the current trend in the pharmaceutical industry to develop chiral methods. This study presents comprehensive strategies for automated rapid chiral HPLC method development including normal-phase, reversed-phase and polar organic phase separation modes.

Some of the newer hydrophilic interaction liquid chromatography (HILIC) columns are reviewed, and experiments performed to categorize columns for optimized separations are described.

Monolithic columns feature both high column efficiency and high column permeability. This article discusses their preparation, applications, and future prospects.

A simple and sensitive high performance liquid chromatography (HPLC) method with ultraviolet (UV) detection was developed to analyse phenylurea herbicides: – monuron, diuron, linuron, metazachlor and metoxuron – in three soft drink brands and tap water.

A mysterious autosampler problem is solved.

A systematic way to develop HPLC methods consists of building up and running a useful set of experiments (DOE, design of experiments) to evaluate the influence of several factors or method conditions on the selectivity of a chromatogram. The crucial part here is the peak tracking between the chromatograms.

The results of a recent survey on high performance liquid chromatography (HPLC) columns shows some interesting changes.

The practical aspects of hollow-fiber liquid-phase microextraction in the three-phase mode (HF3LPME) are described.

A review of HPLC 2011, which took place in Budapest, Hungary in June this year.

The GPC/SEC column is the key component for the separation of macromolecules by size. It enables a conventional HPLC instrument to be used for GPC/SEC analysis.

Flash chromatography is a type of preparative liquid chromatography commonly used in the separation of organic compounds.

The 2011 Manfred Donike award for scientific excellence in sports-doping testing has been presented to Simon Beuck and Nicolas Leuenberger.

Waters has welcomed the Proteomics Core Facility at the Duke University School of Medicine, Durham, North Carolina, USA, into its Centers of Innovation programme.

In this review, practical principles and guidelines for designing LC?LC methods are given.

Incognito draws similarities between advances in car technology and HPLC systems. Better end products have come at the cost of knowledge about how it actually works and can be maintained.

The Singapore Eye Research Institute will advance biomarker candidates that it has identified for chronic inflammatory eye conditions into large-scale clinical trials for validation using AB SCIEX technology.

This work presents a proof of concept that using freshly produced ultrapure water to prepare nano LC eluent does not cause emitter clogging.

Here is a step-by-step approach to investigate the source of ghost peaks in a gradient LC method.

The development of chiral column technology since the review in 2008 is discussed by Thomas Beesley.

This year, many HPLC systems focused on facilitating process development, method transfer, and biological analysis.

In this review, practical principles and guidelines for designing LCxLC methods are given.

This process can improve efficiency in a drug discovery laboratory.

Here's how these new reference standards were characterized.

The Canadian National Research Council?s Institute for Marine Biosciences (NRC-IMB) has signed an agreement with AB SCIEX that will enable researchers worldwide to benefit from the insitute?s experience in the detection and identification of marine and freshwater biotoxins and the creation of certified reference materials.

In 1958 the University of Chicago zapped a simulated primordial earth atmosphere with a series of electrical discharges to demonstrate the way in which organic compounds may have been originally synthesized on Earth. Archived and unreported samples have recently been uncovered and submitted to modern analytical techniques, which have revealed even more abundant amino acid and amine yields than previous experiments.

What fuels our enthusiasm and transforms what on first appearance may seem a boring technique into something that offers inspiration?

The ability to determine vitamin D levels in patients rapidly and reliably is a valuable tool in identification and management of treatment regimes.