Strategies for Rapid Chiral HPLC Method Development for Pharmaceutical Analysis

Article

The Column

ColumnThe Column-04-19-2012
Volume 7
Issue 8
Pages: 2–8

Automated column and mobile phase screening is the current trend in the pharmaceutical industry to develop chiral methods. This study presents comprehensive strategies for automated rapid chiral HPLC method development including normal-phase, reversed-phase and polar organic phase separation modes.

Automated column and mobile phase screening is the current trend in the pharmaceutical industry to develop chiral methods. This study presents comprehensive strategies for automated rapid chiral HPLC method development including normal-phase, reversed-phase and polar organic phase separation modes.

Click here for the full article in The Column

Articles in this issue
Strategies for Rapid Chiral HPLC Method Development for Pharmaceutical Analysis
Strategies for Rapid Chiral HPLC Method Development for Pharmaceutical Analysis
oil-770692-1408605809243.jpg
Oil spill
Autism biomarkers
High Throughput Microchromatography
High Throughput Microchromatography
Ultra-High Resolution Chromatography Using 500 mm UHPLC Columns for Detection Of Hidden Sample Components
Ultra-High Resolution Chromatography Using 500 mm UHPLC Columns for Detection Of Hidden Sample Components
Organic separation
Biofuel-770673-1408605871524.jpg
Biofuel analysis
Recent Videos
An Interview with Martin Medal Winner Fabrice Gritti
An Interview with Martin Medal Winner Fabrice Gritti
Latest Trends in Oligonucleotide Analysis: An Interview with Quang-Dong Bui
Latest Trends in Oligonucleotide Analysis: An Interview with Quang-Dong Bui
Biotech Fluidics: No Troubles with Bubbles®
Biotech Fluidics: No Troubles with Bubbles®
ASMS 2024: An Interview with Peter Horvatovich
ASMS 2024: An Interview with Peter Horvatovich
Toby Astill | Image Credit: © Thermo Fisher Scientific
Thermo Fisher PFAS Expert Discusses Laboratory Testing, Regulatory Partnerships
Rising Stars of Separation Science: David Steiner
Rising Stars of Separation Science: David Steiner
Rising Stars of Separation Science: Shijia Tang
Rising Stars of Separation Science: Shijia Tang
Related Content
Abstract flying dragons on a dark blue background. Technological background for design on the topic of artificial intelligence, neural networks, big data | Image Credit: © LariBat - stock.adobe.com

Predicting Concentration Profiles in Gradient LC Using Neural Networks

Aaron Acevedo
March 13th 2025
Article

Physics-informed neural networks were tested for their capabilities in predicting concentration profiles in gradient liquid chromatography.

The laboratory scientist prepares samples for download to High-performance Liquid Chromatograph Mass Spectrometr. | Image Credit: © Sodel Vladyslav - stock.adobe.com

New Study Investigates Optimizing Extra-Column Band Broadening in Micro-flow Capillary LC

Aaron Acevedo
March 12th 2025
Article

Shimadzu Corporation and Vrije Universiteit Brussel researchers recently investigated how extra-column band broadening (ECBB) can be optimized in micro-flow capillary liquid chromatography.

Scientist injecting a sample via the autosampler of HPLC system. High performance liquid chromatography at chemical laboratory. Developing of pharmaceuticals or vaccine. Biochemistry analysis | Image Credit: © lightpoet - stock.adobe.com

New Study Reviews 2D-LC for Natural Product Analysis

Aaron Acevedo
March 12th 2025
Article

A new review article provides an overview of the use of two-dimensional liquid chromatography (2D-LC) for the quantitative analysis of natural products.

Laptop computer displaying logo of Agilent Technologies | Image Credit: © monticellllo - stock.adobe.com

Agilent Reports Financial Results from Q1 of 2025

Aaron Acevedo
March 11th 2025
Article

The company reported $1.68 billion in revenue at a 25.1% operating margin across its businesses in America, Europe, and Asia.

Ribonucleic acid strands consisting of nucleotides important for protein bio-synthesis entering cell wall | Image Credit: © Christoph Burgstedt - stock.adobe.com

Hydrophilic Interaction Liquid Chromatography for Oligonucleotide Therapeutics: Method Considerations

Szabolcs Fekete;Makda Araya;Balasubrahmanyam Addepalli;Matthew A. Lauber
March 10th 2025
Article

Hydrophilic interaction liquid chromatography (HILIC) has emerged as a promising alternative to traditional ion-pair reversed phase liquid chromatography (IP-RPLC) methods for separating oligonucleotides (ON). This work investigates the application of HILIC to the separation of ON sequence and length variants, duplexes, and single-stranded components.

Scientist moved the vial of sample out from the tray autosampler of the Liquid Chromatography Mass Spectrometer LC/MS after the analysis was complete. | Image Credit: © S. Singha - stock.adobe.com

Matrix Effects on Quantitation in Liquid Chromatography: Sources and Solutions

Dwight R. Stoll
March 10th 2025
Article

The “matrix effect” is one of the sticky details that can make the use of liquid chromatography (LC) for quantitative purposes challenging, even though it is conceptually quite straightforward. In this installment of LC Troubleshooting, Dwight Stoll reviews what is being referred to during talks about “matrix effects”, and discusses some of the potential origins of the phenomenon in practice.