November 13th 2024
The 54th International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC 2025) will be held from Sunday through Thursday, June 15-19, 2025, in Bruges, Belgium.
Facilitated Column Ranking and Selection in Reversed-Phase Liquid Chromatographic Analysis
November 1st 2004In this article, the authors present a column ranking system to facilitate column selection in reversed-phase liquid chromatographic analysis. The system ranks columns based on their similarity to a reference column of choice and its applicability to a real pharmaceutical analysis is demonstrated.
Elevated Temperature HPLC: Principles and Applications to Small Molecules and Biomolecules
October 1st 2004In this article the authors review the use of elevated temperatures in HPLC, and provide examples covering separations of both small molecules and biomolecules. Generic issues of temperature dependence of retention and plate height are discussed, and comparisons are made between temperature gradient and solvent gradient elution. They describe how the use of elevated temperatures allow good chromatographic efficiency to be obtained at flow-rates higher than those optimal at ambient temperature, thus increasing the speed of separation.
Shear-Driven Flow LC: Dispensing with Pumps and Voltage Supplied for Increased Speed and Resolution
May 1st 2004The authors discuss the application of shear-driven chromatography, a technique that depends on viscous drag to propel mobile phase in high-resolution/high-speed liquid chromatography. This method, it is claimed, overcomes the pressure- and voltage-drop limitations of both HPLC and CEC. Early development of the technique is reviewed and updated with the current status of the group's work. Also covered are the challenges faced and opportunities available to interface such systems to MS and UV/vis absorption detectors.
Parallel Chromatography — Double Your Money
May 1st 2004In this month's instalment, the authors cover the topics of column-switching techniques and parallel chromatography for LC?MS?MS methods, and describe the time benefits that can be achieved using such systems. An example set-up is presented and the authors outline the various steps required to perform such analyses routinely.
New Chromatography Columns and Accessories at the 2004 Pittcon Conference, Part I
April 1st 2004In the first of a two-part series, Ron Majors examines new column introductions at Pittcon with special attention to high performance liquid chromatography columns and packings for reversed-, normal- and bonded-phase; ion exchange; ion and size-exclusion chromatography. He also considers speciality HPLC columns.
Success with Evaporative Light-Scattering Detection, Part II: Tips and Techniques
April 1st 2004Following on from a previous instalment, this month's column assesses evaporative light-scattering detection as a complementary detection mode and builds upon the previous discussion in terms of detector applications.