Choice of Buffer for the Analysis of Basic Peptides in Reversed-Phase HPLC

Article

LCGC Europe

LCGC EuropeLCGC Europe-05-01-2005
Volume 18
Issue 5
Pages: 290–297

This article examines the results of chromatographic performance when using different buffers. A study of the origins of the differences in peak shape obtained when using varying buffers–additives is presented with regard to the analysis of basic peptides in reversed-phase HPLC.

External Link - article-159008.pdf

Articles in this issue
The Bermuda Triangle
Preparative Chiral SFC as a Green Technology for Rapid Access to Enantiopurity in Pharmaceutical Process Research
A Question of Time (and Date)
What’s in a Method
PeakMaster — A Freeware Simulator of Capillary Zone Electrophoresis
Choice of Buffer for the Analysis of Basic Peptides in Reversed-Phase HPLC
Recent Videos
Toby Astill | Image Credit: © Thermo Fisher Scientific
Related Content
RAFA 2024 Highlights: Contemporary Food Contamination Analysis Using Chromatography

RAFA 2024 Highlights: Contemporary Food Contamination Analysis Using Chromatography

Alasdair Matheson
November 18th 2024
Article

A series of lectures focusing on emerging analytical techniques used to analyse food contamination took place on Wednesday 6 November 2024 at RAFA 2024 in Prague, Czech Republic. The session included new approaches for analysing per- and polyfluoroalkyl substances (PFAS), polychlorinated alkanes (PCAS), Mineral Oil Hydrocarbons (MOH), and short- and medium-chain chlorinated paraffins (SCCPs and MCCPs).

Best of the Week: Emerging Chromatography Leader, Bladder Cancer Research, and HPLC 2025

Best of the Week: Emerging Chromatography Leader, Bladder Cancer Research, and HPLC 2025

Aaron Acevedo
November 15th 2024
Article

Here is some of the most popular content posted on LCGC International this week.

Canals of Bruges, Belgium | Image Credit: © gqxue - stock.adobe.com

In Bruges: HPLC 2025

Gert Desmet;Deirdre Cabooter;Frédéric Lynen;Sebastiaan Eeltink;Ken Broeckhoven
November 13th 2024
Article

The 54th International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC 2025) will be held from Sunday through Thursday, June 15-19, 2025, in Bruges, Belgium.

Generate a list of online forums or discussion groups for additional support (Generated with AI) | Image Credit: © ngstock - stock.adobe.com

Recent Trends and Developments in Sample Preparation

Will Wetzel
November 13th 2024
Article

LCGC International spoke with various corporate personalities about trends and developments in sample preparation.

Advancing Bladder Cancer Research with Mass Spectrometry: A FeMS Interview with Marta Relvas-Santos

Advancing Bladder Cancer Research with Mass Spectrometry: A FeMS Interview with Marta Relvas-Santos

Aaron Acevedo
November 12th 2024
Article

LCGC International interviewed FeMS Empowerment Award winner Marta Relvas-Santos on her use of mass spectrometry to identify potential biomarkers and therapies for bladder cancer. She also shared insights on her work with FeMS and advice for fellow scientists.

Molecule of mRNA, 3D illustration (Generated with AI) | Image Credit: © Dr_Microbe - stock.adobe.com

mRNA Characterization, from 5’ Cap to Poly (A): What Ion-Pair Reversed-Phase Liquid Chromatography (IP-RPLC) Analysis Can Tell You

Roxana Eggleston-Rangel
November 12th 2024
Article

This article will discuss an ion-pair reversed-phase liquid chromatography mass spectrometry (IP-RPLC–MS) workflow for the characterization of three messenger RNA (mRNA) key critical quality attributes: 5’ Cap, ORF, and poly(A) tail.