Adjusting Conditions for a Routine Reversed-Phase HPLC Assay, Part 1: Changing the Column

Article

LCGC Europe

LCGC EuropeLCGC Europe-04-01-2005
Volume 18
Issue 4
Pages: 196–203

In the first part of this series, the authors look at method modification from a scientific and regulatory standpoint with regard to changing a column and the operating parameters.

External Link - article-152910.pdf

Articles in this issue
Split Peaks — A Case Study
CDS and LIMS Integration
Adjusting Conditions for a Routine Reversed-Phase HPLC Assay, Part 1: Changing the Column
Visual Presentation of Data by Means of Box Plots
Accelerated and Automated Extraction of Hexavalent Chromium from Solid Waste Materials Coupled with Ion Chromatography Determination
Emerging Technologies in the MS Arsenal
Recent Videos
An Interview with Martin Medal Winner Fabrice Gritti
An Interview with Martin Medal Winner Fabrice Gritti
Biotech Fluidics: No Troubles with Bubbles®
Biotech Fluidics: No Troubles with Bubbles®
ASMS 2024: An Interview with Peter Horvatovich
ASMS 2024: An Interview with Peter Horvatovich
Toby Astill | Image Credit: © Thermo Fisher Scientific
Thermo Fisher PFAS Expert Discusses Laboratory Testing, Regulatory Partnerships
Rising Stars of Separation Science: David Steiner
Rising Stars of Separation Science: David Steiner
Rising Stars of Separation Science: Zhuoheng Zhou
Rising Stars of Separation Science: Zhuoheng Zhou
Related Content
Abstract flying dragons on a dark blue background. Technological background for design on the topic of artificial intelligence, neural networks, big data | Image Credit: © LariBat - stock.adobe.com

Predicting Concentration Profiles in Gradient LC Using Neural Networks

Aaron Acevedo
March 13th 2025
Article

Physics-informed neural networks were tested for their capabilities in predicting concentration profiles in gradient liquid chromatography.

Pittcon 2025: Chris Palmer Discusses Halogen Bonding in HPLC

Pittcon 2025: Chris Palmer Discusses Halogen Bonding in HPLC

Will Wetzel
March 13th 2025
Article

In this video interview from Pittcon, we ask Christopher Palmer about developing novel polymeric materials for microscale liquid phase separations, as well as some of the current trends and challenges he is observing in chromatography.

The laboratory scientist prepares samples for download to High-performance Liquid Chromatograph Mass Spectrometr. | Image Credit: © Sodel Vladyslav - stock.adobe.com

New Study Investigates Optimizing Extra-Column Band Broadening in Micro-flow Capillary LC

Aaron Acevedo
March 12th 2025
Article

Shimadzu Corporation and Vrije Universiteit Brussel researchers recently investigated how extra-column band broadening (ECBB) can be optimized in micro-flow capillary liquid chromatography.

Scientist injecting a sample via the autosampler of HPLC system. High performance liquid chromatography at chemical laboratory. Developing of pharmaceuticals or vaccine. Biochemistry analysis | Image Credit: © lightpoet - stock.adobe.com

New Study Reviews 2D-LC for Natural Product Analysis

Aaron Acevedo
March 12th 2025
Article

A new review article provides an overview of the use of two-dimensional liquid chromatography (2D-LC) for the quantitative analysis of natural products.

Laptop computer displaying logo of Agilent Technologies | Image Credit: © monticellllo - stock.adobe.com

Agilent Reports Financial Results from Q1 of 2025

Aaron Acevedo
March 11th 2025
Article

The company reported $1.68 billion in revenue at a 25.1% operating margin across its businesses in America, Europe, and Asia.

Ribonucleic acid strands consisting of nucleotides important for protein bio-synthesis entering cell wall | Image Credit: © Christoph Burgstedt - stock.adobe.com

Hydrophilic Interaction Liquid Chromatography for Oligonucleotide Therapeutics: Method Considerations

Szabolcs Fekete;Makda Araya;Balasubrahmanyam Addepalli;Matthew A. Lauber
March 10th 2025
Article

Hydrophilic interaction liquid chromatography (HILIC) has emerged as a promising alternative to traditional ion-pair reversed phase liquid chromatography (IP-RPLC) methods for separating oligonucleotides (ON). This work investigates the application of HILIC to the separation of ON sequence and length variants, duplexes, and single-stranded components.