Introduction: HPLC Column Technology - State of the Art

Hydrophilic interaction liquid chromatography (HILIC) has emerged as a promising alternative to traditional ion-pair reversed phase liquid chromatography (IP-RPLC) methods for separating oligonucleotides (ON). This work investigates the application of HILIC to the separation of ON sequence and length variants, duplexes, and single-stranded components.

The “matrix effect” is one of the sticky details that can make the use of liquid chromatography (LC) for quantitative purposes challenging, even though it is conceptually quite straightforward. In this installment of LC Troubleshooting, Dwight Stoll reviews what is being referred to during talks about “matrix effects”, and discusses some of the potential origins of the phenomenon in practice.

During Waters Corporation’s 2025 Investor Day, the company’s top executives highlighted the company’s growth and hints at future points of development.

Here is some of the most popular content posted on LCGC International this week.

In the final section of our interview with Christopher Pohl, he imparts knowledge to the next generation of aspiring separation scientists.

In this month's edition of the LCGC Blog, Emanuela Gionfriddo discusses chromatography resources that can help make the field more accessible to newcomers and veterans alike.