Screening Procedure for “Non Banned” Drugs
November 23rd 2015A new screening procedure has been developed for the detection of common “non banned” drugs used by athletes. The method targets multiple pharmacologically active compounds by liquid chromatography-electrospray ionization coupled to tandem mass spectrometry (LC-ESI-MS-MS).
The LCGC Blog: Column Overload in Gas Chromatography with Vacuum Ultraviolet Detection
November 10th 2015Column overload is a very commonly encountered issue in GC for beginners. Changes in peak symmetry, generally observed as peak fronting, can be subtle in the sharp peaks generated by GC, but the result can be significant shifts in retention times, loss of resolution, and error in peak integration.
Characterizing Therapeutic Monoclonal Antibodies
November 5th 2015Accounting for the inherent heterogeneity of monoclonal antibodies is essential to ensure production of consistent and safe therapeutics and, in light of this, there is an array of chromatographic and supporting regulatory systems. Robust characterization and analysis of monoclonal antibodies is becoming a fast and straightforward process thanks to the myriad technological advances underpinning this rapidly evolving area.
The First 50 Years of HPLC—A Parallel Journey
November 1st 2015Ron Majors’s personal recollections on the first 50 years of column development-with perspectives on what was going on behind the scenes, particularly during the exciting early days-including a summary of his early publications that contributed to development of HPLC column technology.
The first 50 years of HPLC—A Parallel Journey
November 1st 2015Ron Majors’s personal recollections on the first 50 years of column development-with perspectives on what was going on behind the scenes, particularly during the exciting early days-including a summary of his early publications that contributed to development of HPLC column technology.
What Is “Dead” Volume and Why Should Chromatographers Worry About It?
November 1st 2015The term dead volume often comes up in chromatography discussions and literature. This month we address the nature of this phenomenon, when it can become a problem that affects chromatographic results, and how to understand and take control of it.
The Current Status and Future of LC Column Technology: What the Experts Are Saying
November 1st 2015A summary of the results from a survey of 14 leading HPLC–UHPLC column experts is presented, covering the state of sub-2-?m porous particles, superficially porous (core–shell) particles, silica monoliths, and polymeric monoliths.
Split-Vent Traps: GC’s Dirty Secret
November 1st 2015In gas chromatography (GC) split injection systems, as much as 99% or more of the injected sample never enters the column: It is released downstream of the inlet where it can encounter and possibly perturb precision gas control devices. The split-vent trap acts to prevent or at least moderate such effects. This month’s instalment addresses the operation and maintenance of split-vent traps.