All News
Volumetric Absorptive Microsampling for Hepcidin Peptide Extraction from Whole Blood
May 1st 2016Whole blood analysis is an emerging trend in the field of bioanalysis. We developed a fast and simple protocol to extract and analyze a peptide, hepcidin, from whole blood. Sampling and extraction were carried out using volumetric absorptive microsampling (VAMS), a novel blood collection device that allows the sampling of a known blood volume independently from hematocrit. The composition of the extraction medium was optimized using an experimental design to get the most intense signal of hepcidin, considering different organic solvents and acidic additives.
Challenges & Benefits of Using SFC
April 27th 2016Ted Szczerba, Technical Director of Chromatography and SFC Separations at Regis Technologies, overviews incorporating Supercritical Fluid Chromatography (SFC) into his separations lab. With over 30 years experience working with chiral compounds and over 10 years running SFC, he assesses the value of the technique versus the drawbacks of what is available in the field today. Learn about a typical screening day and this industry veteran's advice on what is needed for an effective separations process.
Development Partners for BioPharma
April 27th 2016Your current contract laboratory and instrument provider may be familiar and comfortable, but, maintaining the status quo doesn’t lead to greater rewards. In fact, it often involves putting up with less-than-desired results. Join us in this executive summary to hear how Ajinomoto Althea develops unsurpassed solutions for biopharma companies, and how a team at Thermo Fisher Scientific has challenged the status quo to deliver uncompromised UHPLC.
Can SBWC Replace HPLC in Pharmaceutical Analysis?
April 25th 2016Subcritical water chromatography (SBWC) is seen as an increasingly enticing prospect to replace high performance liquid chromatography (HPLC) in industrial settings because of its low costs and environmental impact. Yu Yang of East Carolina University, in Greenville, North Carolina, USA, has conducted research into this process for two decades. He recently spoke to Lewis Botcherby of The Column about his work, why companies should consider SBWC, and its role in pharmaceutical analysis.
Investigating Alzheimer’s Using UHPLC–MS
April 25th 2016Researchers from Heilongjiang University of Chinese Medicine, China, have used ultrahigh-performance liquid chromatography coupled to mass spectrometry and metabolomics to investigate the metabolic networks of Alzheimer’s disease, identifying potential biomarkers and biochemical mechanisms.
A Stoichiometric Displacement Model for Proteins in Reversed-Phase LC
April 25th 2016The literature on reversed-phase liquid chromatography (LC) of proteins is reasonably well developed, but not taught in the college classroom much. Kevin Schug therefore wants to focus on a stoichiometric displacement model for reversed-phase LC of proteins and why it is particularly insightful from a practical perspective.
Magnetic Nanoparticles for Solid-Phase Extraction
April 21st 2016Magnetic nanoparticles (m-NPs) are becoming important in analytical chemistry as sorbents in dispersive solid-phase extraction (d-SPE) because they simplify the extraction process and save time as a result of their isolation from the sample matrix by an external magnetic field. Many synthetic processes have been developed to fabricate these nanomaterials and an additional coating step is usually included at the end of the synthesis to improve their stability and to avoid the formation of agglomerates. A wide variety of coatings have been used for this purpose to improve selectivity. This manuscript provides an overview of the different synthesis methods, coatings, and applications of m-NPs as sorbents in d-SPE.
Practical Advances in SFC for the Purification of Pharmaceutical Molecules
April 21st 2016Recent progress in supercritical fluid chromatography (SFC) instrumentation has led to renewed interest in the technique as a powerful tool for chiral and achiral separations of pharmaceutical molecules, for both analytical and preparative purposes. The “green” aspect of the technique and low running costs make SFC technology particularly attractive for preparative chromatography because it considerably reduces the consumption of organic solvents. These factors led to a revised strategy for purification and to a general interest in evaluating possible extensions for the application of packed SFC (pSFC). The results of this extensive evaluation have led to the establishment of SFC platforms for preparative achiral purifications as a standard practice, alongside its use for preparative chiral separations.
