The LCGC Blog: HPLC Diagnostic Skills–Noisy Baselines
February 13th 2019Just as medical practitioners are able to discern worrying features from a variety of medical physics devices (electrocardiogram, electroencephalogram, ultrasound, for example), we need to develop the skill to identify worrying symptoms from our HPLC instrument output.
The LCGC Blog: Would Your Process Benefit from Multivariate Optimization?
February 6th 2019If you have a method or process that involves a number of different variables, multivariate optimization approaches can provide a faster route to optimum conditions and can lead to a more reliable outcome than using a one-factor-at-a-time approach. With a little study and practice, students and researchers can apply these optimization techniques, even if a complete understanding of the underlying statistical treatments is not immediately apparent.
Tips & Tricks GPC/SEC: System Peaks or Ghost Peaks in GPC/SEC
February 6th 2019Extraneous peaks, unrelated to the solute to be characterized, are quite common in gel permeation chromatography/size-exclusion chromatography (GPC/SEC), especially when refractive index (RI) detection is used. This instalment of Tips & Tricks explains why system or ghost peaks appear and how to minimize their appearance.
Innovations in Ion-Exchange Chromatography for mAb Variant Analysis
February 6th 2019A surge in the development and commercialization of monoclonal antibody (mAb)-based ther-apeutics has driven the requirement for accurate and reproducible analytical methods for pro-tein characterization. Monoclonal antibodies are popular biologic drug candidates, but are sus-ceptible to a myriad of modifications during manufacture and complex degradation pathways during purification and storage, often leading to distinct charge variants that require character-ization and quantification. Ion‑exchange liquid chromatography (IEX) is a well-accepted and widely used technique to separate various mAb charge variant species for the sake of charac-terization and profiling. With the most recent advances in analytical technologies, IEX can be used to help ensure the selection of stable and efficacious mAb drug candidates, from discov-ery through manufacturing.
GCxGC: From Research to Routine
February 1st 2019Two-dimensional gas chromatography (GCxGC) is becoming the technique of choice for analysis of highly complex samples such as petroleum, pharmaceuticals, biological materials, food, flavors, and fragrances. Here, we explain how GCxGC works and provide examples that illustrate its advantages.
Data Integrity Focus, Part II: Using Data Process Mapping to Identify Integrity Gaps
February 1st 2019Data integrity is paramount when working in a regulated environment. Data process mapping is an excellent way to identify and mitigate data gaps and record vulnerabilities in a chromatographic process. This approach is simple and practical.
Optimizing Sensitivity in GC–FID Analysis
February 1st 2019It is often possible to achieve better sensitivity and lower limits of detection and quantitation using standard gas chromatography instrumentation. Paying attention to fundamental variables-like sample solvent, initial oven temperature hold, carrier gas operating mode, and thermal gradients- can lead to much improved method performance.
Going Green in Pharmaceutical Analysis
February 1st 2019LCGC Asia Pacific spoke to Yong Liu and Adam Socia from MSD about the cost-saving benefits of implementing green chromatography in the pharmaceutical sector, the importance of analytical method volume intensity (AMVI), and effective practices to reduce solvent consumption and replace harmful solvents, including supercritical fluid chromatography (SFC), fast chromatography, and “cocktail chromatography”.
The LCGC Blog: UV Detection for HPLC—Fundamental Principle, Practical Implications
January 17th 2019I’ve written several times on the operational principles and variables used in UV detection, but I also get asked questions on the basic theory of UV detection, and this time I’m going to address some of those FAQs to give a little more background theory as well as some operational tips and tricks.