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Click the title above to open The Column January 09, 2014 North American issue, Volume 10, Number 1, in an interactive PDF format.

Click the title above to open The Column January 09, 2014 Europe & Asia issue, Volume 10, Number 1, in an interactive PDF format.

The Australian Patent Office has granted a patent related to W. R. Grace & Company?s (Columbia, Maryland) Reveleris flash chromatography system. The patent concerns the use of evaporative light scattering detection (ELSD) with flash chromatography.

Chris Reddy, a marine geochemist at the Woods Hole Oceanographic Institution (Massachusetts, USA), has been selected to receive the 2014 Clair C. Patterson Award from the Geochemical Society. The award recognizes one scientist a year who has made an innovative breakthrough in environmental geochemistry in service to society.

Click here to view the full European E-newsletter from January 2.

A look at what's in store for chromatographers at Pittcon 2014, which will be held at McCormick Place South, Chicago, USA, from 2–6 March 2014.

What good is that big, ugly peak at the beginning of the chromatogram?

This tutorial on reversed-phase LC explains the role of solvent, chain conformation, solute position, and retention dynamics.

This month's instalment tracks the progress of helium, hydrogen, and nitrogen carrier gases as they start their journey through a GC system.

Entirely new sample preparation technologies continue to be introduced, mainly in the academic sector. Some of these technologies will undoubtedly stay in the academic laboratory. However, some new technologies may "cross the chasm" and eventually become a standard laboratory procedure. This instalment will examine some of those methods.

The jubilee 20th International Symposium on Separation Sciences (ISSS 2014) will take place at the Hotel DAP in Prague, Czech Republic, from 30?August to 2 September 2014.

Click the title above to open the LCGC Europe 2014 regular issue, Vol 27 No , in an interactive PDF format.

Click the title above to open the LCGC North America January 2014 regular issue, Vol 32 No 1, in an interactive PDF format.

The head of the Chinese Academy of Sciences Interdisciplinary Research Center on Biology and Chemistry (Beijing, China), Junying Yuan, has received an Agilent Thought Leader Award. Agilent Technologies (California, USA) has given the award in recognition of Yuan?s scientific contributions to cellular biology and the understanding of neurodegenerative diseases.

Waters Corporation (Massachusetts, USA) has announced that Chief Financial Officer John Ornell intends to move to a reduced workload and resign from 1 February 2014. Ornell will continue to work for the company in an advisory capacity on a part-time basis. Eugene G. Cassis, currently Corporate Vice President of Worldwide Business Development and Investor Relations, will serve as the company?s interim Chief Financial Officer from 1 February 2014.

The head of the Chinese Academy of Sciences Interdisciplinary Research Center on Biology and Chemistry (Beijing, China), Junying Yuan, has received an Agilent Thought Leader Award. Agilent Technologies (California, USA) has given the award in recognition of Yuan?s scientific contributions to cellular biology and the understanding of neurodegenerative diseases.

There has been much focus within the industry of late on ways of improving the way in which HPLC method development is carried out - which has led to a more widespread adoption of modelling and optimisation software combined with dedicated method development platforms from the major instrument vendors.

Waters Corporation (Massachusetts, USA) has announced that Chief Financial Officer John Ornell intends to move to a reduced workload and resign from 1 February 2014. Ornell will continue to work for the company in an advisory capacity on a part-time basis. Eugene G. Cassis, currently Corporate Vice President of Worldwide Business Development and Investor Relations, will serve as the company?s interim Chief Financial Officer from 1 February 2014.

Click here to view the full European E-newsletter from December 19.

Click here to view the full European E-newsletter from December 19.

Based on Wyatt Technology?s legacy of multi-angle light scattering (MALS) detectors, the DAWN HELEOS-II is the MALS detector with the greatest sensitivity for absolute molecular weight and size determinations of polymers and biopolymers in solution. The DAWN may be connected in series to any chromatographic system in order to determine absolute molar masses without the use of reference standards or column calibration.

Proteins, monoclonal antibodies (mAbs) and antibody-drug conjugates (ADCs) are powerful therapeutic agents. Because of their high molecular complexity, however, biopharmaceuticals may be quite sensitive to changes in the manufacturing processes that can lead to considerable micro-heterogeneity. As a result, a panel of separation techniques based on both liquid chromatography and electrophoresis has been used for their characterization and comparability studies. In terms of detection, mass spectrometry (MS) plays a pivotal role in the structural elucidation of biopharmaceuticals, because it offers an additional degree of separation by mass/charge ratio, greatly facilitating the characterization of variants. This two-part web seminar will discuss possibilities and limitations of chromatographic techniques and mass spectrometry detection for the physico-chemical characterization of biopharmaceutical compounds. Part II will focus on mass spectrometry techniques for the physico-chemical characterization of mAbs and related compounds. Classical MS analysis comprising intact mAb mass measurements, LC-MS analyses in reducing conditions or after deglycosylation treatment, and peptide mapping will be presented. The focus will be on enzymatic treatments that generate mAb fragments of smaller size that are more readily analyzable by MS techniques. In this presentation, some new trends in MS including native MS and ion mobility?MS (IM-MS) approaches will be also discussed. Finally, the benefits of high resolution native MS analysis will be explained.

Proteins, monoclonal antibodies (mAbs) and antibody-drug conjugates (ADCs) are powerful therapeutic agents. Because of their high molecular complexity, however, biopharmaceuticals may be quite sensitive to changes in the manufacturing processes that can lead to considerable micro-heterogeneity. As a result, a panel of separation techniques based on both liquid chromatography and electrophoresis has been used for their characterization and comparability studies. In terms of detection, mass spectrometry (MS) plays a pivotal role in the structural elucidation of biopharmaceuticals, because it offers an additional degree of separation by mass/charge ratio, greatly facilitating the characterization of variants. This two-part web seminar will discuss possibilities and limitations of chromatographic techniques and mass spectrometry detection for the physico-chemical characterization of biopharmaceutical compounds. Part I will focus on chromatographic techniques used for the physico-chemical characterization of biopharmaceutical compounds. Historically, ion exchange chromatography (IEX) and size exclusion chromatography (SEC) were the reference techniques. In this presentation, some new trends in IEX and SEC will be explained. However, because reversed-phase liquid chromatography is naturally more compatible with MS, the talk will focus on the potential of state-of-the-art UHPLC instruments and reversed-phase LC columns (sub-2?m wide-pore porous particles or core-shell particles) for biopharmaceutical characterization.

Click here to view the complete E-Separation Solutions newsletter from December 17, 2013.

Editors Series' : Integrating On-Line Sample Preparation and HPLC Separations with Triple Quadrupole Mass Spectrometry for Ultratrace Quantitative Analysis of Small Molecules from Complex Matrices

LCGC Pittcon 2014 product Review-Product2

Residues of chili pepper (Capsicum) have been found on pottery dating back 2000 years in southern Mexico, according to a study published in PLOS ONE.1 The New World Archeological Foundation (NWAF) first excavated the pottery (thought to date from 400 BCE–300 CE) from Chiapa de Corzo between 1955 and 1963.

Click here to view the complete E-Separation Solutions newsletter from December 12, 2013.

Flow injection analysis (FIA) is a powerful technique for quantitative analysis, especially in conjunction with mass spectrometry (MS) detection. Kevin Schug discusses how FIA can be combined with MS using electrospray ionization and describes how the technique can be used for analytes such as binding constant determination, multipoint instrument response calibrations, and removing the dependence of measured analyze concentrations on matrix effects.