Liquid Chromatography (LC/HPLC)

Why is SFC becoming increasingly more important as a sustainable analytical technique?

Recombinant adeno-associated viruses (rAAV) have been the subject of much scientific investigation as their use in gene therapy enters the mainstream.

In a talk at ASMS 2023, Rebecca L. Beres of the University of North Carolina at Chapel Hill focused on two types of analysis methods: liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS) and a liquid chromatography, ion mobility spectrometry, and mass spectrometry (LC–IMS-MS) platform.

Researchers have developed polyacrylamide hydrophilic stationary phases with adjustable performance. The innovative approach allows for customized separation capabilities, enhancing the efficiency and versatility of chromatographic techniques. The findings open up new possibilities for natural product chemistry and pharmaceutical research.

Researchers achieve a breakthrough in countercurrent chromatography, successfully separating three types of sanshools from Zanthoxylum bungeanum oleoresin, paving the way for enhanced purification and isolation of valuable compounds.

Several factors strongly influence separation speed, including the pressure available to drive the separation, column temperature, particle size, and column length. Developing efficient methods to improve speed while not sacrificing accuracy require an understanding of the abovementioned variables and analysis time.

Nonspecific adsorption leads to poor peak shapes and poor performance from HPLC systems, but coating-enabling technologies can serve as a potential solution. We explain how.

What you need to know about pharmaceutical regulations as an analytical chemist is provided here, including an explanation about how working in a Good Manufacturing Practice (GMP) environment can yield positive results.

Characterizing lipid nanoparticles (LNPs) is a developing delivery modality in biotherapeutic analysis. We address some of the current challenges and opportunities in this field, including an examination of the most common tools used to characterize LNPs.

Are analytical chemists really operating sustainable laboratories, or are they being too quick in marketing their “green” laboratory credentials?

This study suggests a 2D-LC screening method to evaluate peak purity and ensure specificity in pharmaceutical analysis, thereby promoting the safe production of medicines by detecting active pharmaceutical ingredients (API) and related substances.

Building up knowledge of how different factors affect resolving power from a theoretical point of view, as well as what changes are possible within practical constraints, is powerful when we are confronted with a separation that needs to be improved, or when an existing separation is not performing as expected.

Nonspecific adsorption leads to poor peak shapes and poor performance from HPLC systems, but coating-enabling technologies can serve as a potential solution. We explain how.

The latest developments and challenges of using liquid chromatography (LC) for copolymer analysis are discussed.

Metal organic frameworks (MOFs) and ionic liquids (ILs) combine to form a powerful gas chromatography stationary phase, enabling improved separation properties and expanded application possibilities. This innovative system offers enhanced separation capabilities and paves the way for advancements in gas chromatography analysis.

Superficially porous particle (SPP) column technology is leveraged to optimize high-throughput HPLC methods for small-molecule drug discovery, resulting in improved efficiency and reduced runtime.

An analytical team has successfully developed a novel strategy for the separation of sanshools from Zanthoxylum bungeanum oleoresin using countercurrent chromatography, providing valuable insights for the separation of compounds with similar chemical properties.

A selective adsorption material was sought to capture and possibly remove ochratoxin A (OTA) from food and environmental samples prior to high performance liquid chromatography (HPLC) analysis.

Researchers in Iran utilize graphene oxide aerogels to significantly improve the extraction and analysis of risperidone from plasma samples, offering enhanced accuracy and efficiency in pharmaceutical analysis.

A novel system combining hydrogel-modified human umbilical vein endothelial cell membrane chromatography with high-performance liquid chromatography-mass spectrometry revolutionizes the screening and purification of active ingredients in herbal medicines.

A new publication introduces a groundbreaking automated system for high-throughput mass spectrometry analysis, reshaping the future of biotherapeutic characterization.

Rosuvastatin, lovastatin, and simvastatin were determined for dosage quality control in pharmaceutical formulations using hybrid micellar liquid chromatography (HMLC).

A recent study has introduced comprehensive quality control procedures for large-scale plasma proteomics analyses. The research provides valuable insights into optimizing performance, enhancing reproducibility, and ensuring quantitative accuracy in proteomics investigations.

Using intact protein separation (IPS) together with traditional peptide separation (PS) enhanced detection for LC–MS/MS better than either method could do independently.

A cutting-edge technique combining droplet microfluidics and mass spectrometry revolutionizes monoclonal antibody screening, enabling rapid and high-throughput analysis while setting a new standard for efficient clone selection in biopharmaceutical development.

Automation and high-throughput MALDI mass spectrometry revolutionize peptide metabolic stability screening, allowing for rapid analysis of a large number of samples and minimizing solvent waste.

A new study introduces a comprehensive two-dimensional liquid chromatography coupled with tandem mass spectrometry (2D-LC–MS/MS) method for the analysis of 112 pesticides in corn-based products. The method exhibits high precision, lower limit of quantification values, and successful detection of trace levels of pesticides in real samples, offering promise for the analysis of complex matrices.

Eighty drugs prohibited in athletic competition were analyzed, with seven samples returning adverse findings compared to previous methods.

The eggs of the yellow-legged gull and Audouin’s gull and the blood of the greater flamingo were studied to assess their exposure to perfluoroalkyl substances (PFAS) as bioindicators of chemical pollution.

A biomarker with significant clinical and analytical potential in an enzyme present in many human diseases was determined at higher efficiency using liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS).