Liquid Chromatography (LC/HPLC)

Researchers have developed a novel liquid chromatography–mass spectrometry method for analyzing sialic acid linkage in N-linked glycopeptides, offering insights into glycosylation patterns.

A new method determines gas-polydimethylsiloxane (PDMS) distribution constants of key Cannabis terpenes and terpenoids, providing insights into their behavior and for optimizing SPME methods for analysis of these compounds.

A researcher has derived an expression for peak width in liquid chromatography with exponential retention and linear program preceded by an isocratic hold, providing valuable insights for enhancing chromatographic separations.

Mono-type, blending-type, coating-type, and bonding-type chromatographic packings based on chiral metal-organic frameworks (CMOFs) were analyzed regarding their effectiveness in enantioseparation.

We interviewed an AI program (ChatGPT) for LCGC North America asking questions about AI and its role in various applications for separation science to include data analysis, and high performance liquid chromatography (HPLC), hydrophilic-interaction chromatography (HILIC), reversed-phase liquid chromatography (RPLC), liquid chromatography–mass spectrometry (LC–MS), gas chromatography–mass spectrometry (GC–MS), high resolution mass spectrometry (HRIM–MS), high resolution tandem mass spectrometry (HRMS/MS), and related topics.

Webinar Date/Time: Tue, Jun 13, 2023 11:30 AM EDT

New statistical framework developed by researchers at the University of California in Riverside allows confident identification of isomeric molecules by mass spectrometry, even when no unique mass-to-charge peaks are generated between the isomeric pairs.

This month we interview Juan L. Benedé from the University of Valencia in Spain, about his work developing microextraction approaches, based on the use of sorbent materials, for application to complex matrices, and his focus on the determination of disease biomarkers in biofluids.

The evolution of biomolecule analysis is discussed in this article.

At Pittcon 2023, LCGC spoke with Jim Grinias of Rowan University. This segment is the full-length version of the interview that was conducted at the conference.

The 51st International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC 2023) will be held at the Conference Center Düsseldorf (CCD) in Germany, from 18–22 June 2023.

What parameters can influence an siRNA separation?

How to enhance the sensitivity, specificity, and speed of metabolite measurement using TIMS, which in turn helps to accelerate the pace of metabolomics research.

A team of researchers from the University of Barcelona has developed a new method for the selective extraction of monosaccharide anhydrides from sediment samples, which is crucial for their analysis.

The workshop will take place 28–29 June 2023 in Liège, Belgium.

A newly proposed concept in liquid chromatography (LC) positions spherical particles, either individually or stacked, in pockets forming microgrooves that act as perfectly aligned chromatographic columns.

A new study reports the development a highly sensitive LC–MS/MS method to measure salivary melatonin levels. The method has a lower limit of the measuring interval of 0.8 pg/mL, making it the most sensitive method for quantifying melatonin in saliva.

A recent study has found that using only seven points across an LC–MS/MS peak can provide sufficient accuracy and precision for drug quantitation studies in drug discovery and development, challenging the "rule-of-thumb" of using at least 15 points.

A new article discusses the benefits and drawbacks of RPLC and RPLC×RPLC chromatography techniques. It provides recommendations for selecting the best approach for analyzing small pharmaceuticals and peptides.

Samples of the prefrontal cortex, striatum, and hippocampus of rats were analyzed by LC–MS to assess brain tissue response to combined consumption of ethanol and cocaine.

A recent study analyzed the composition and metabolism of steroidal alkaloids in Solanum nigrum fruits of different maturities and identified steroidal glycoalkaloids and steroidal alkaloids, providing a foundation for further research on biosynthesis and discovery of new steroidal alkaloid anticancer drugs.

A new published study introduces a simple and fast way to differentiate between animal species based on their hair. The in-sample digestion method coupled with mass spectrometry analysis was successfully tested on multiple species.

A new study has identified potential quality-markers for Eucommiae Folium (Duzhongye), a traditional Chinese medicine, using UHPLC–MS/MS analysis, and has recommended isoquercitrin as a new quality-marker candidate.

Trace amounts of polychlorinated biphenyls (PCBs) in a river and lake in China were detected using a custom coating for stir bar sorptive extraction (SBSE) followed by high performance liquid chromatography with diode array detection (HPLC-DAD).

The reliability of column chromatography for GC–MS analysis of hopanes was tested in oil samples of three different types, using neutral alumina as a solid-phase adsorbent and a Pasteur pipette as a separation device.

A new approach for identifying isomeric and isobaric metabolites has been developed using liquid chromatography (LC) with high-resolution ion mobility spectrometry (IMS) and cryogenic infrared (IR) spectroscopy, allowing for faster, more accurate, and cost-effective metabolomics workflows.

Researchers have developed a new approach to extract chloramphenicol (CAP) from milk and chicken samples using magnetic nanofluid-based deep eutectic solvents (DES), which was combined with high performance liquid chromatography-ultraviolet (HPLC-UV) analysis. The method was found to be low-cost, green, efficient, and effective in detecting even trace amounts of CAP.

Scientists have developed a magnetic ionic liquid hypercrosslinked polymer composite for use in magnetic solid-phase extraction (MSPE) of non-steroidal anti-inflammatory drugs (NSAIDs), which was coupled with high-performance liquid chromatography (HPLC) for the detection of trace amounts of NSAIDs in water and urine samples.

This article compares the performance of wide-pore silica monolithic, sub-2µm FPP, and SPP columns, addresses the question of whether 1000 Å or 400 Å SPP columns are more suitable for reversed-phase LC-type protein separations, and presents a kinetic performance comparison of different columns.

A micro-pillar array format for mapping the proteome of human stem cell-derived liver organoids using timsTOF–MS is presented.