Liquid Chromatography (LC/HPLC)

Liquid chromatography (LC) is a platform technology amenable to portable and “at-site” or deployable applications. This has awoken end-users to new possibilities and potential cost savings and process improvements.

At HPLC 2023, Deirdre Cabooter, a professor at KU Leuven in Belgium, gave a talk titled, “Detailed Investigation of Intra-Particle Diffusion in Liquid Chromatography”. She spoke with LCGC on her current and future research in fundamentals.

At HPLC 2023, John Langley, a professor at the University of Southampton in Southampton, UK, presented a talk called "(R)evolution of Hyphenations, Through Open-Access RP-HPLC–MS, Open-Access UHPSFC–MS and 2D-LC–IMS-MS in Academia". He spoke with LCGC on the importance of open-access hyphenation, and how to implement it in your research.

A novel countercurrent chromatography solvent system, utilizing deep eutectic solvents, offers efficient separation of trans-crocetin and cis-crocetin from Gardenia jasminoides Ellis, enabling high-purity isolation in a single process.

Scientists from the University of Utah in Salt Lake City, Utah, created a new technique to characterize lipids, specifically glycolipids, using ion mobility spectrometry–mass spectrometry (IMS-MS).

Untargeted analysis using liquid chromatography with tandem mass spectrometry (LC–MS/MS) chemical profiling is a valuable tool, but care needs to be taken with experimental design.

Scientists used supercritical fluid chromatography (SFC) and ultrahigh-pressure liquid chromatography (UHPLC)—both coupled with high-resolution mass spectrometry (HRMS)—to analyze various types of chlorinated paraffins (CPs) in fish oil-based dietary supplements.

Mobile phase degassing has become very useful for troubleshooting problems with pumps and detection.

How can low-resolution mass spectrometry (LRMS) and high-resolution (HR) MS work together to provide unambiguous results in clinical and forensic toxicology?

The methodologies demonstrated here open the way to fully compliant at-line monitoring of monoclonal antibody quality attributes.

As chromatography technology evolves, our use of it should change also. This article outlines a number of protective and troubleshooting methods for LC and how these approaches have changed over time.

An overview of GLP regulations and related public standards.

Results from our recent survey on sample preparation techniques uncover trends in the field over the last generation and their impact going forwards.

Alasdair Matheson spoke to Jacob de Boer of Vrije Universiteit Amsterdam about a recent innovative research project investigating per- and polyfluoroalkyl substances (PFAS) in fast food packaging.

Researchers have developed a multitargeted internal calibration method for precise quantification of chronic kidney disease (CKD)-related endogenous metabolites using liquid chromatography–mass spectrometry (LC–MS).

At the Cannabis Sciences Conference, Zacariah Hildenbrand held a talk which focused on various analytical methods and how they affect the cannabis production process.

Okadaic acid (OA) is considered a marine toxin with distributed hydroxyl, polycyclic ether, and carboxyl groups, easily accumulated in shellfish and known to cause food poisoning in humans.

In a recent study, researchers from China presented a novel approach that uses a supramolecular solvent (SUPRAS) method for detecting bioactive compounds.

This review of pharmaceutical analysis in the last three years includes three categories of chiral liquid chromatography stationary phases: saccharides, macrocyclic substances, and porous organic materials.

Scientists from the National Institute of Pharmaceutical Education and Research (NIPER) in India used a variety of techniques to characterize erdafitinib and its various products.

Researchers from Hebei University in China have developed and characterized three novel peptide chiral stationary phases (CSPs) for enantiomeric separation, demonstrating their potential in high performance liquid chromatography (HPLC).

Researchers from Corteva Agriscience tested a new approach for separating and quantifying small molecule structure elucidation.

A pair of scientists from Gdańsk University of Technology (GUT) in Gdańsk, Poland, used the method to analyze the presence of antibiotics in animal products like milk and eggs.

We spoke with Ibraam Mikhail, a postdoctoral research fellow (analytical chemistry) at the Australian Centre for Research on Separation Science (ACROSS), University of Tasmania, Australia, about his work developing a multiwavelength absorbance detector that uses pulsed-deep ultraviolet light-emitting diodes (DUV-LEDs), and how this detector performs compared to other standard detectors.

Small modifications to the way measurements are approached can enable reductions in the waste generated and carbon dioxide produced during an analysis.

The 27th International Symposium on Separation Sciences (ISSS 2023) will be held 24–27 September 2023 in Cluj-Napoca, Romania. Here’s a sneak preview of what attendees can look forwards to.

Pop quiz—can you define resolution? Would you be surprised at the number of correct answers? Which one is the best for chromatographers? In this LCGC Blog, we look at the various ways resolution can be interpreted scientifically.

Brazilian scientists are testing a system to detect endocrine disruptors in wastewater samples to help improve public health.

Researchers from the University of Washington have begun testing a new system to fight antimicrobial resistance by combining liquid chromatography (LC) with ion mobility (IM) separation.

Optimal reaction conditions for the desired deethylation were found to be mild, using only water and oxygen at or close to ambient temperatures.