Ion Chromatography

Joining us for a discussion on ion chromatography this month are experts from Dionex, Metrohm and Merck.

Joining us for a discussion on ion chromatography this month are experts from Dionex, Metrohm and Merck.

Metrohm Application Note

The fully automated combustion ion chromatography (CIC) system presented here combines a highly efficient combustion system with the separation power of ion chromatography (IC). CIC allows for the simultaneous, speciated trace analysis of halide (F, Cl, Br and I) and sulphur compounds (as sulphate) from sub-ppm to percent levels in any nonaqueous sample matrix.

Methacholine chloride is a synthetic analogue of the neurotransmitter acetylcholine used to assess bronchial asthma of subjects in respiratory function labs and epidemiological field studies.

The basics of IC are reviewed and recent advances that may extend this technology to new applications are discussed.

Metrohm Application Note

The fully automated combustion ion chromatography (CIC) system presented here combines a highly efficient combustion system with the separation power of ion chromatography (IC).

Methacholine chloride is a synthetic analogue of the neurotransmitter acetylcholine used to assess bronchial asthma of subjects in respiratory function labs and epidemiological field studies (1).

This article considers some examples of uses of ion chromatography.

Separation of Chromium (III) and Chromium (VI) by Ion Chromatography - Dionex Apps Note

Organic acids are present in many media and play various crucial roles. Low molecular mass organic acids (LMMOAs) have been researched extensively in many areas, such as food chemistry, as these acids affect taste, shelf-life, and food safety (1–3); agriculture, ecosystems, and environmental science, as these acids act as key components in mechanisms that some plants use to cope with environmental stress (4) and fertilizer release (5); biotechnology, to better understand and optimize fermentation processes (6,7); and biomedical research (8–11). Recently, LMMOAs were found to have inhibitory effects on the bioconversion efficiencies of lignocelluloses to ethanol (12).

Metrohm Application Note

Ion chromatography (IC) is a similar technique to HPLC except that the detector of choice is usually a conductivity detector.

This article describes the use of combined ion chromatography-mass spectrometry (IC–MS) and ion chromatography-inductively coupled plasma mass spectrometry (IC-ICP–MS) to analyse potentially harmful compounds.

This article describes the use of combined ion chromatography-mass spectrometry (IC–MS) and ion chromatography-inductively coupled plasma mass spectrometry (IC-ICP–MS) to analyze potentially harmful compounds.

Determination of Fluoride in Acidulated Phosphate Topical Solutions Using Reagent-Free Ion Chromatography-Dionex Application Note

Determination of Parts-Per-Trillion Concentrations of Strontium by Pre-Concentration with Ion Chromatography and Suppressed Conductivity Detection- Dionex Application Note

Perflourinated organic acids are ubiquitous and found at relatively low concentrations in the environment (1). Trifluoroacetic acid (TFA) is the persistent atmospheric degradation product of hydrofluorocarbons (HFCs) that are increasingly used as an alternative to banned, ozone-damaging chlorofluorocarbons (CFCs). However, debate surrounds the use of HFCs because of their potential to contribute to global warming and demonstrated toxicity to the environment (2). TFA is also widely used in pharmaceutical and biotechnology purification processes. It is crucial to monitor for TFA in environmental risk assessment and in products intended for human use. TFA can be measured by gas chromatography (GC) after sample preparation and chemical derivatization (3), ion chromatography (IC) (4), and capillary electrophoresis (5). This paper describes an IC-MS method to separate TFA from common anions based on Reagent-Freeâ„¢ IC (RFICâ„¢) technology with sensitive and selective mass spectrometric detection.

Run a difficult food sample on your IC and you stand a big chance that you will wreck the column. Of course, you can waste a lot of time on tedious sample preparation steps to eliminate undesired matrix components. Or you can go for Metrohm's automated compact stopped-flow dialysis providing optimum separation while protecting your column from detrimental compounds.

Ionic liquids have the potential to replacing classic organic solvents in numerous applications.

This month, Chromatography Online's Technology Forum looks at the topic of HPLC/ Ion Chromatography and the trends and issues surrounding it. Joining us for this discussion is Chris Pohl of Dionex Corporation, Jody Clark of Selerity Technologies, Scott Anderson and Laura Kaepplinger of Grace Davison Discovery Sciences, and Doug McCabe of Waters Corporation.

This article describes a straightforward ion chromatographic method that uses isocratic elution and pulsed amperometric detection (PAD) to sensitively determine water-soluble polyols and sugar alcohols as well as mono-, di- and oligosaccharides in essential and nonessential foodstuffs. While carbohydrate determination of most foodstuffs requires only minimal sample pretreatment such as dilution and filtration, samples with interfering matrices such as protein-containing dairy products have to be dialyzed prior to injection.

Cefepime is a fourth generation cephalosporin (1). During preparation and storage, cefepime degrades by release of the N-methylpyrrolidine (NMP) side chain and opening of the beta-lactam ring. An NMP concentration increase will directly affect the potency of the active component of the drug. Therefore, it is critical to determine the amount of NMP in cefepime. The US Pharmacopeia (USP) monograph specifies the limit of NMP to <0.3% in cefepime hydrochloride and <1% in cefepime for injection (2,3). The latter is a dry mixture of cefepime hydrochloride and L-arginine. The current USP method uses cation-exchange chromatography with non-suppressed conductivity detection to determine the limit of NMP in cefepime. There are several disadvantages to this method, such as the ~3-4 h time required per injection, a lack of retention time stability for NMP in standard and sample solutions, and a lack of sensitivity. In this paper, we describe an improved method using a hydrophilic, carboxylate-functionalized cation..

This month's Technology Forum looks at the topic of HPLC/Ion Chromatography Focus and the trends and issues surrounding it. Joining us for this discussion is Chris Pohl, VP and Chief Science Officer at Dionex Corporation, Larry Tucker from Metrohm-Peak, and Yuichi Fusho of Shodex.

Ion chromatography (IC) is well suited for the analysis of a variety of inorganic and organic anions and cations. There is an additional dimension to an experiment using the technique as a result of the need to deal with changes in the ionic strength of the solution as the analyte materials are exchanged.

Ion chromatography (IC) is well suited for the analysis of a variety of inorganic and organic anions and cations.