Biopharmaceuticals and Protein Analysis

Researchers explore the benefits of a mobile affinity selection chromatography (MASC) luminon assay method to assess the quality of therapeutic monoclonal antibodies.

Scientists recently used laser desorption ionization mass spectrometry (LDI-MS) to detect toxic alkaloids in traditional Chinese medicines (TCM).

Two scientists from Baskent University in Ankara, Turkey analyzed multiple proteins in cream formulation using reverse-phase high-performance liquid chromatography.

Scientists from Zhejiang, China recently conducted experiments meant to properly characterize sucrose ester isomers from tobacco samples.

We present formulation and delivery of gene therapy products, focusing on adeno-associated virus (AAV) formulations. We also discuss the analytical technologies most used to characterize these products.

This month we interview Zhuoheng Zhou from the Vrije Universiteit Brussel in Belgium, about his focus on advancing instrumental and column technologies for modern chromatography and its applications in novel biopharmaceutical modalities and proteomics. We also spoke to him about his work developing a protocol to help with the design of polymer monolithic capillary columns.

A recent study published in the Journal of American Society of Mass Spectrometry introduced a new method for characterizing nonpolar lipids (1).

Scientists from the University of Utah in Salt Lake City, Utah, created a new technique to characterize lipids, specifically glycolipids, using ion mobility spectrometry–mass spectrometry (IMS-MS).

The comprehensive characterization of multispecific antibodies is essential to the development of safe and efficacious cancer treatments.

The methodologies demonstrated here open the way to fully compliant at-line monitoring of monoclonal antibody quality attributes.

Researchers from Texas A&M University presented a new N–H aziridination method designed to target carbon–carbon double bonds in nonpolar sterol lipids.

In a recent study, the research team introduces a new software tool for proteomic analysis that can analyze both ESI and MALDI mass spectral data.

The phenolic compounds of Chrysanthemum morifolium have recently been separated in a study meant to identify their medical benefits.

In a recent study, a new instrument configuration suggests an alternative method for investigating polypeptides.

Researchers in China studied a new process for measuring aberrant metabolites.

To further understand what role ion chromatography (IC) plays in urine analysis, scientists investigated urine pretreatment techniques and how IC systems are designed and developed to measure various chemicals.

HRPs, while vital to creating drugs meant to fight serious diseases, are very difficult to quantify. To streamline the process, a group of scientists used a new approach for analyzing HRPs using MSPE and HPLC-UV.

To gain a better understanding of phosphatidylinositols, scientists used different LC–MS/MS techniques to find classifications to use in identifying further species of Pls.

When used appropriately in RPLC, ion pairing agents can increase the separation and retention of charged analytes. But should they be explored in other modes of HPLC as well?

An overview of different approaches for the qualitative and quantitative analysis of polysorbates.

In this interview, we explore the significance of this groundbreaking technology and its impact on mass spectrometry with Aaron Robitaille, Director of Marketing for Mass Spectrometry at Thermo Fisher Scientific in San Jose, California.

During the North American Chemical Residue Workshop, scientists discussed predicting liquid chromatographic retention times to identify contaminants.

Researchers compare the separation efficiency of olefins and paraffins using stationary phases containing silver(I) ions in ionic liquid (IL) and polymeric ionic liquid (PIL) matrices for gas chromatography techniques.

Researchers have introduced a novel primary method utilizing gas chromatography–isotope dilution infrared spectrometry (GC–IDIR) for accurate peptide purity analysis.

Researchers have developed a robust method utilizing high-performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS) to accurately quantify amino acids in plasma, making it a valuable tool for clinical diagnostics and research in amino acid metabolism.

Scientists from the Pacific Northwest National Laboratory are using high-resolution ion mobility spectrometry-mass spectrometry to identify unknown compounds.

Researchers have discovered that phospholipids form doubly charged lipid-metal ion complexes during electrospray ionization (ESI), providing valuable insights into the analysis of phospholipid structures.

A research team has developed a new approach for selecting precursor ions in tandem mass spectrometry (MS/MS) experiments called DiffN, which increases the likelihood of biomarker discovery and provides a promising tool for lipidomic analyses.

New research reports on the development of a groundbreaking strategy that utilizes metal oxide-nanochannel arrays as templates for the in-situ growth of metal-organic frameworks (MOFs), revolutionizing the field of protein isolation, offering promising potential for high-efficiency protein recovery from complex biological samples.

In Alejandro Cifuentes's keynote lecture at HPLC 2023, he discussed the research he and his team have done to explore how foodomics can be effective in combating Alzheimer's disease.