Biopharmaceuticals and Protein Analysis

New types of smart proteolytic samplers were tested for their effectiveness in liquid chromatography-mass spectrometry (LC–MS)-based protein quantification.

A new solid-phase microextraction-based technique has been developed for extracting triazole residues from environmental samples including water and juice.

Here is some of the most popular content posted on LCGC International this week.

Hydrophilic interaction liquid chromatography (HILIC) has emerged as a promising alternative to traditional ion-pair reversed phase liquid chromatography (IP-RPLC) methods for separating oligonucleotides (ON). This work investigates the application of HILIC to the separation of ON sequence and length variants, duplexes, and single-stranded components.

Here is some of the most popular content posted on LCGC International this week.

Here is some of the most popular content posted on LCGC International this week.

Guangdong University of Technology researchers used a gas chromatography-based method to detect nonylphenol isomers in sewage and wastewater sludge.

Scientists from Bristol Myers Squibb developed a method using supercritical fluid chromatography for chiral bioanalysis.

Here is some of the most popular content posted on LCGC International this week.

Supercritical fluid chromatography (SFC) has been optimized for the analysis of 5-, 10-, 15-, and 18-mer oligonucleotides (ONs) and evaluated for its effectiveness in separating impurities from ONs.

Researchers have produced a review of chromatography media and purification processes for handling complex and super-large biomolecules.

A review of recent trends and remaining challenges in bispecific antibody (bsAb) production has been published.

Here is some of the most popular content posted on LCGC International this week.

A new method has been created for determining N-nitrosamines (NAs) in pharmaceutical preparations using liquid chromatography with tandem mass spectrometry.

Ion chromatography can be utilized in various ways to analyze halogens and similar substances within inorganic matrices.

New methods have been developed to determine polar emerging contaminants with hydrophilic interaction liquid chromatography (HILIC) techniques.

Scientists from the Henan Academy of Sciences recently developed a new technique for the determination of hydroxyl polycyclic aromatic hydrocarbons (OH-PAHs) from urine.

A new process for tracking targeted and non-targeted fluorinated residuals using liquid chromatography–high-resolution mass spectrometry (LC–HRMS) has been created.

Scientists from Nanjing University recently developed a new solid-phase microextraction (SPME)-based system for detecting nitrated polycyclic aromatic hydrocarbons (nitro-PAHs) in aquatic environments.

Here is some of the most popular content posted on LCGC International this week.

Scientists recently studied how different factors affect antisense oligonucleotides (ASOs) under hydrophilic interaction liquid chromatography (HILIC) conditions.

Spanish scientists recently tested flow injection analysis–mass spectrometry (FIA–MS) and its effectiveness in detecting fraudulent substances in Coleus forskohlii food supplements.

Shihezi University scientists recently developed a new imprinted structure to separate acteoside (ACT) from other substances within Cistance tubulosa, or “desert ginseng.”

Harbin University of Commerce and Northeast Forestry University scientists led efforts to create new means of analyzing components in traditional Chinese medicine.

A recent joint study between the University of Ferra and YMC ChromaCon led an effort to a new approach to more efficiently purify oligonucleotides.

University of Oslo scientists combined electromembrane extraction (EME) and flow injection-MS/MS (FI–MS/MS) to determine polar endogeneous metabolites in human plasma.

An overview of different approaches for the qualitative and quantitative analysis of polysorbates.

In this article, the use of comprehensive two-dimensional liquid chromatography (LC×LC) coupled to mass spectrometry (MS) for characterizing glycosylation of therapeutic enzymes is presented.

New gene therapy modalities, such as CRISPR guide RNA (single guide ribonucleic acid [sgRNA]) and messenger RNA (mRNA), continue to make progress in both primate and first-in-human trials. As this progress builds, the industry remains accountable for characterizing these molecules to meet the requirements of regulatory authorities.

How is mD-LC–MS changing the way we evaluate HPLC peaks and assess performance?