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Analysis of Monoclonal Antibodies and Their Fragments by SEC Coupled with HRAM-MSThe biopharmaceutical industry continues to focus on the development of biotherapeutic monoclonal antibody (mAbs) drugs. In this article, the compatibility of SEC coupled with HRAM–MS for the analysis of mAbs is demonstrated.
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Determination of Very Low Abundance Diagnostic Proteins in Serum Using Immunocapture LC–MS/MSThere is growing interest in the determination of endogenous proteins in biological samples for diagnostic purposes, because a concentration increase or decrease of such proteins can allows us to monitor the state of a pathological condition such as cancer. Immunocapture LC–MS/MS analysis combines the workflow of conventional immunological assays with LC–MS analysis. This article describes typical challenges, such as cross reactivity and the mass spectrometer’s dynamic range, as well as the advantages of isoform differentiation and multiplexing.
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Determination of Very Low Abundance Diagnostic Proteins in Serum Using Immunocapture LC–MS/MSThere is growing interest in the determination of endogenous proteins in biological samples for diagnostic purposes, because a concentration increase or decrease of such proteins can allows us to monitor the state of a pathological condition such as cancer. Immunocapture LC–MS/MS analysis combines the workflow of conventional immunological assays with LC–MS analysis. This article describes typical challenges, such as cross reactivity and the mass spectrometer’s dynamic range, as well as the advantages of isoform differentiation and multiplexing.
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Retention Time Prediction Based on Molecular Structure in Pharmaceutical Method Development: A PerspectiveThe principal aim of this work was to provide a perspective with practical utility in streamlining the chromatographic method development in pharmaceutical industries based upon predicting the chromatographic retention times from molecular structures. Workflows were suggested with a focus on reversed-phase LC, IC, and HILIC as the three major techniques. Unlike HILIC, retention prediction in both reversed-phase LC and IC can benefit from the maturity of these techniques and the transparency of their retention mechanisms. In reversed-phase LC the solute coefficients in the hydrophobic subtraction model and in IC the a and b values in the linear solvent strength model can be the subject of modelling with their subsequent use in retention prediction. A workflow for HILIC can be based on the design of experiments approach, to account for all major contributors to the retention mechanism, and direct correlation of experimental retention times to the molecular descriptors.
Latest:
Retention Time Prediction Based on Molecular Structure in Pharmaceutical Method Development: A PerspectiveThe principal aim of this work was to provide a perspective with practical utility in streamlining the chromatographic method development in pharmaceutical industries based upon predicting the chromatographic retention times from molecular structures. Workflows were suggested with a focus on reversed-phase LC, IC, and HILIC as the three major techniques. Unlike HILIC, retention prediction in both reversed-phase LC and IC can benefit from the maturity of these techniques and the transparency of their retention mechanisms. In reversed-phase LC the solute coefficients in the hydrophobic subtraction model and in IC the a and b values in the linear solvent strength model can be the subject of modelling with their subsequent use in retention prediction. A workflow for HILIC can be based on the design of experiments approach, to account for all major contributors to the retention mechanism, and direct correlation of experimental retention times to the molecular descriptors.
Latest:
Retention Time Prediction Based on Molecular Structure in Pharmaceutical Method Development: A PerspectiveThe principal aim of this work was to provide a perspective with practical utility in streamlining the chromatographic method development in pharmaceutical industries based upon predicting the chromatographic retention times from molecular structures. Workflows were suggested with a focus on reversed-phase LC, IC, and HILIC as the three major techniques. Unlike HILIC, retention prediction in both reversed-phase LC and IC can benefit from the maturity of these techniques and the transparency of their retention mechanisms. In reversed-phase LC the solute coefficients in the hydrophobic subtraction model and in IC the a and b values in the linear solvent strength model can be the subject of modelling with their subsequent use in retention prediction. A workflow for HILIC can be based on the design of experiments approach, to account for all major contributors to the retention mechanism, and direct correlation of experimental retention times to the molecular descriptors.
Latest:
Retention Time Prediction Based on Molecular Structure in Pharmaceutical Method Development: A PerspectiveThe principal aim of this work was to provide a perspective with practical utility in streamlining the chromatographic method development in pharmaceutical industries based upon predicting the chromatographic retention times from molecular structures. Workflows were suggested with a focus on reversed-phase LC, IC, and HILIC as the three major techniques. Unlike HILIC, retention prediction in both reversed-phase LC and IC can benefit from the maturity of these techniques and the transparency of their retention mechanisms. In reversed-phase LC the solute coefficients in the hydrophobic subtraction model and in IC the a and b values in the linear solvent strength model can be the subject of modelling with their subsequent use in retention prediction. A workflow for HILIC can be based on the design of experiments approach, to account for all major contributors to the retention mechanism, and direct correlation of experimental retention times to the molecular descriptors.
Latest:
Retention Time Prediction Based on Molecular Structure in Pharmaceutical Method Development: A PerspectiveThe principal aim of this work was to provide a perspective with practical utility in streamlining the chromatographic method development in pharmaceutical industries based upon predicting the chromatographic retention times from molecular structures. Workflows were suggested with a focus on reversed-phase LC, IC, and HILIC as the three major techniques. Unlike HILIC, retention prediction in both reversed-phase LC and IC can benefit from the maturity of these techniques and the transparency of their retention mechanisms. In reversed-phase LC the solute coefficients in the hydrophobic subtraction model and in IC the a and b values in the linear solvent strength model can be the subject of modelling with their subsequent use in retention prediction. A workflow for HILIC can be based on the design of experiments approach, to account for all major contributors to the retention mechanism, and direct correlation of experimental retention times to the molecular descriptors.
Latest:
Asymmetric Flow Field-Flow Fractionation Analysis of Engineered Nanoparticles in Mouthwashes and Their Interaction with SalivaThe evaluation of the oral uptake of engineered nanoparticles (ENPs) contained in personal care products like mouthwashes is of great relevance to estimate the potential hazards and the toxicity of engineered nanomaterials (ENMs). Various experiments were performed while two commercially available mouthwash products (named M1 and M2) were selected as samples of interest. Asymmetric flow field‑flow fractionation (AF4) was chosen and optimized as the particle separation technique and two detectors were on-line coupled while dynamic light scattering (DLS) was used for evaluation and signals obtained by ultraviolet–visible (UV–vis) detection at 254 nm were used to gather additional information about the fate of the ENPs.
Latest:
Asymmetric Flow Field-Flow Fractionation Analysis of Engineered Nanoparticles in Mouthwashes and Their Interaction with SalivaThe evaluation of the oral uptake of engineered nanoparticles (ENPs) contained in personal care products like mouthwashes is of great relevance to estimate the potential hazards and the toxicity of engineered nanomaterials (ENMs). Various experiments were performed while two commercially available mouthwash products (named M1 and M2) were selected as samples of interest. Asymmetric flow field‑flow fractionation (AF4) was chosen and optimized as the particle separation technique and two detectors were on-line coupled while dynamic light scattering (DLS) was used for evaluation and signals obtained by ultraviolet–visible (UV–vis) detection at 254 nm were used to gather additional information about the fate of the ENPs.
Latest:
Asymmetric Flow Field-Flow Fractionation Analysis of Engineered Nanoparticles in Mouthwashes and Their Interaction with SalivaThe evaluation of the oral uptake of engineered nanoparticles (ENPs) contained in personal care products like mouthwashes is of great relevance to estimate the potential hazards and the toxicity of engineered nanomaterials (ENMs). Various experiments were performed while two commercially available mouthwash products (named M1 and M2) were selected as samples of interest. Asymmetric flow field‑flow fractionation (AF4) was chosen and optimized as the particle separation technique and two detectors were on-line coupled while dynamic light scattering (DLS) was used for evaluation and signals obtained by ultraviolet–visible (UV–vis) detection at 254 nm were used to gather additional information about the fate of the ENPs.
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Direct Analysis of Amino Acids by HILIC–ESI-MSHydrophilic interaction liquid chromatography coupled to electrospray ionization mass spectrometry (HILIC–ESI-MS) has been established as a method to separate and quantify polar and ionic analytes in a direct way for two decades. HILIC separation is based on the polarity of analytes, so the more polar analytes have stronger retention on a HILIC column.
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Analysis of Organophosphates in Lithium Ion Battery Electrolytes by HILIC–ESI-MSNew separation techniques for the analysis of polar and ionic analytes have aroused great interest in the field of metabolomics and environmental investigation in the past two decades. Hydrophilic interaction liquid chromatography (HILIC) is a promising tool to address this challenge. HILIC separation is based on the polarity of analytes, which generally show stronger retention with increasing polarity according to the HILIC separation mechanism. Furthermore, the high content of organic solvent in the mobile phase leads to good ionization properties in the electrospray ionization (ESI), and consequently enhances the detection sensitivity by hyphenated mass spectrometry (MS) detector.
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Imagine a World of ChromatographyThis year, the ISC’s theme is “Imagine a world of Chromatography." Here, we will see conference attendees discuss a wide variety of disciplines where chromatography plays a crucial role.
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Sensitive, Rapid Estimation of Moxidectin in Cattle Hair by LC–MS-MSValidation of this rapid bioanalytical method for the determination of moxidectin in cattle hair demonstrated that the method is accurate, reliable, and reproducible.
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Sensitive, Rapid Estimation of Moxidectin in Cattle Hair by LC–MS-MSValidation of this rapid bioanalytical method for the determination of moxidectin in cattle hair demonstrated that the method is accurate, reliable, and reproducible.
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A Simple Analysis of 4-Methylimidazole Using Automated Solid-Phase Extraction and High Performance Liquid Chromatography with MS-MS and MS–SIM DetectionThis work will demonstrate a simple methodology using automated solid-phase extraction (SPE) and HPLC coupled with mass spectrometric detection. This work will demonstrate a simple methodology using automated solid-phase extraction (SPE) and HPLC coupled with mass spectrometric detection. This work will demonstrate a simple methodology using automated solid-phase extraction (SPE) and HPLC coupled with mass spectrometric detection.
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Characterization and Determination of Irganox 1076 and 1010 in Polyethylene Using Thermal Desorption and Reactive Pyrolysis-GC-MSThis application note details a GC-MS-based analytical method for the qualitative and quantitative determination of Irganox 1076 and 1010 in polyethylene.
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New Technologies That Accelerate Persistent Organic Pollutant (POP) Sample PreparationAccelerated solvent extraction (ASE) is widely viewed as a better alternative to GC–MS when analyzing persistent organic pollutants (POPs). The authors explain why new technological developments offer many benefits and accelerate POP sample preparation processes.
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Visualize Your Process Streams in Real-Time with MS for Rapid Process DevelopmentThe Diablo 5000A RTGA-MS allows the process stream to be seen in real-time and provides quantitative data with reliable mass information. The result is visualization.
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A Simple Analysis of 4-Methylimidazole Using Automated Solid-Phase Extraction and High Performance Liquid Chromatography with MS-MS and MS–SIM DetectionThis work will demonstrate a simple methodology using automated solid-phase extraction (SPE) and HPLC coupled with mass spectrometric detection. This work will demonstrate a simple methodology using automated solid-phase extraction (SPE) and HPLC coupled with mass spectrometric detection. This work will demonstrate a simple methodology using automated solid-phase extraction (SPE) and HPLC coupled with mass spectrometric detection.
Latest:
A Simple Analysis of 4-Methylimidazole Using Automated Solid-Phase Extraction and High Performance Liquid Chromatography with MS-MS and MS–SIM DetectionThis work will demonstrate a simple methodology using automated solid-phase extraction (SPE) and HPLC coupled with mass spectrometric detection. This work will demonstrate a simple methodology using automated solid-phase extraction (SPE) and HPLC coupled with mass spectrometric detection. This work will demonstrate a simple methodology using automated solid-phase extraction (SPE) and HPLC coupled with mass spectrometric detection.
Latest:
A Simple Analysis of 4-Methylimidazole Using Automated Solid-Phase Extraction and High Performance Liquid Chromatography with MS-MS and MS–SIM DetectionThis work will demonstrate a simple methodology using automated solid-phase extraction (SPE) and HPLC coupled with mass spectrometric detection. This work will demonstrate a simple methodology using automated solid-phase extraction (SPE) and HPLC coupled with mass spectrometric detection. This work will demonstrate a simple methodology using automated solid-phase extraction (SPE) and HPLC coupled with mass spectrometric detection.
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FPLC versus Analytical HPLC: Two Methods, One Origin, Many DifferencesFast protein liquid chromatography (FPLC) and high performance liquid chromatography (HPLC): two liquid chromatographic methods in direct comparison. This article discusses the differences between the two techniques with particular emphasis on the requirements of their respective analytes.
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Applying LC with Low-Resolution MS/ MS and Subsequent Library Search for Reliable Compound Identification in Systematic Toxicological AnalysisSystematic toxicological analysis is an important step in medicolegal investigations of death, poisoning, and drug use. The primary goal is the detection and confirmation of potentially toxic compounds in evidence. This article describes a workflow using nontargeted liquid chromatography–tandem mass spectrometry (LC–MS/MS) for reliable compound identification.
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Applying LC with Low-Resolution MS/ MS and Subsequent Library Search for Reliable Compound Identification in Systematic Toxicological AnalysisSystematic toxicological analysis is an important step in medicolegal investigations of death, poisoning, and drug use. The primary goal is the detection and confirmation of potentially toxic compounds in evidence. This article describes a workflow using nontargeted liquid chromatography–tandem mass spectrometry (LC–MS/MS) for reliable compound identification.
