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Modifying the Metal Surfaces in HPLC Systems and Columns to Prevent Analyte Adsorption and Other Deleterious EffectsA novel surface modification technology has been developed to reduce interactions between analytes and metal surfaces in HPLC instruments and columns. We demonstrate the impact of this technology on peak symmetry, peak area, and injection-to-injection and column-to-column reproducibility for several metal-sensitive analytes.
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Ion Mobility–Mass Spectrometry (IM–MS): Enhancing Performance of Analytical MethodsModern ion mobility–mass spectrometry (IM–MS) is a key separation technology for detailed molecular characterization studies and also as part of emerging data acquisition strategies for demanding small molecule and several applications. Here is what you need to know.
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Ion Mobility–Mass Spectrometry for Food Analysis: An UpdateIn food analysis, many different biological matrices are investigated containing numerous compounds that can interfere with liquid chromatographyÐmass spectrometry (LC–MS) analysis. To overcome the challenges that arise with these highly complex matrices, the additional separation of analytes and matrix compounds complementing chromatographic separation is becoming more significant. In this article, the potential of IM-MS to increase selectivity and for additional identity confirmation is investigated. An extensive evaluation of IM-MS instruments was performed on a broad test set of food safety contaminants. The tested IM-MS platforms were DMS, TWIMS, low field DTIMS, and TIMS. CCS data were determined using the different instruments, and the ability to separate isomers and compounds of interest from sample matrix in the IM dimension was explored.
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Microflow LC–MS-MS: The Past, the Present, and the Path ForwardMicroflow LC–MS-MS has seen a surge of attention, development, and popularity among research scientists and bioanalysts over the last few years. The potential of this technology to provide better sensitivity, less solvent waste, near-zero dead volume, and high through-put are a big part of this renewed interest. However, microflow LC techniques are hardly a new idea. More than 40 years ago, in 1974, a group at Nagoya University in Japan first developed a microcolumn liquid chromatography system, elements of which can be found in today’s commercial products. With the advances in technology over the last several years, development and implementation of this technique have been kicked into high gear. In this article, we discuss the history of microflow LC–MS-MS, the current state of the art, and where the future might lead for this rapidly growing technology.
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Analysis of Active Cannabis Compounds in Edible Food Products: Gummy Bears and BrowniesPotency testing in marijuana-infused edibles is a problematic task due to the complexity of the matrices. The concentration of active ingredients in edibles can range from a few ppm to 3.5% (1). In this application, active cannabinoid compounds were extracted from gummy bears (and also brownies, results not shown), followed by HPLC analysis.
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Novel Sample Preparation Methods for Analyzing Pain Management Drugs in Complex MatricesThe importance of sample preparation for analyzing pain management drugs in different matrices
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Is the Art of HPLC Method Development Dead?CHROMacademy's Tutor: Scott Fletcher discusses whether HPLC method development has become a dying art.
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Screening for Hundreds of Pesticide Residues Using a GC/Q-TOF with an Exact Mass Pesticide Database in FoodThis note discusses how to rapidly screen, identify, and generate quantitative information for pesticide residues in food with the Agilent 7200 GC/Q-TOF with Agilent MassHunter Qualitative Analysis All Ions workflow.
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Screening for Hundreds of Pesticide Residues Using a GC/Q-TOF with an Exact Mass Pesticide Database in FoodThis note discusses how to rapidly screen, identify, and generate quantitative information for pesticide residues in food with the Agilent 7200 GC/Q-TOF with Agilent MassHunter Qualitative Analysis All Ions workflow.
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Mass Spectrometry in Late Development and QC: Practical Considerations for Multi-Attribute Monitoring and BeyondAs a result of the pharmaceutical cGMP for the 21st century and quality by design (QbD) initiatives championed by regulators, the biopharmaceutical industry has been looking for ways to introduce more automated and higher information content analyses into manufacturing, late-development, and quality control (QC). Mass spectrometry (MS-) based attribute monitoring assays have been proposed as key tools to provide the sensitivity, throughput, selectivity, and flexibility required for monitoring critical product and process attributes for biopharmaceutical production and release. Two analytical workflows, subunit multi-attribute monitoring (MAM) and peptide MAM, have emerged to dominate this discussion, and this article is intended to reflect on the active debates over the needs, challenges, and practical limitations for adopting MS-based attribute monitoring for late-development and QC.
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Quantitative Comparison of Hormones in Drinking Water Between Low and High Resolution Mass SpectrometryThe quantitative performance of the latest generation of high-resolution instruments is comparable to that of a triple quadrupole MS, even though different scanning modes are used. Higher-resolution instrumentation also allows flexibility concerning compound identification because the experiment can be set up for targeted quantitation, screening, or both. In an Orbitrap-based instrument, the parallel reaction monitoring (PRM) mode performs most closely to a triple quadrupole mass analyzer using selected reaction monitoring (SRM) mode. This study looks at the performance of an Orbitrap-based LC–MS method for EPA Method 539.
