Improving SFC Chiral Separations by Employing a New Chlorinated Polysaccharide Chiral Stationary Phase: The RegisPack CLA-1
The Application Notebook
As the number of chiral columns and chiral separations has grown, it has become difficult to organize the information for use in developing new methods. The success of chiral method development depends on the introduction of appropriate columns. The RegisPack? polysaccharide-based chiral stationary phase (CSP) is most successful in achieving a majority of chiral separations. However, there are cases in which this proven chiral selector leads to partial or no separation. Excellent separations using the newly introduced RegisPack? CLA-1 CSP have been obtained for a range of compounds, either by improving or complementing RegisPack's selectivity.
As the number of chiral columns and chiral separations has grown, it has become difficult to organize the information for use in developing new methods. The success of chiral method development depends on the introduction of appropriate columns. The RegisPack® polysaccharide-based chiral stationary phase (CSP) is most successful in achieving a majority of chiral separations. However, there are cases in which this proven chiral selector leads to partial or no separation. Excellent separations using the newly introduced RegisPack® CLA-1 CSP have been obtained for a range of compounds, either by improving or complementing RegisPack's selectivity.
This study used the well-established RegisPack® CSP and the new chlorinated RegisPack® CLA-1 CSP. Both phases were based on 5-µm silica. Columns were both 25 cm x 4.6 mm in size.
Figure 1: Chromatogram Set 1. Sample: Propafenone. Mobile Phase: CO2/IPA (70/30) + 0.5% DEA. Flow Rate: 4.0 mL/min. Temp: 40 °C. Pressure: 125 bar. UV: 254 nm.
The RegisPack® and RegisPack® CLA-1 are coated with tris-(3,5-dimethylphenyl) carbamate of amylose and tris-(5-chloro-2-methylphenyl) carbamate of amylose, respectively.
Table I: Various other samples screened showing an improvement in separation with RegisPack® CLA-1.
This study investigated several compounds that had poor or no separation on a RegisPack® column. They were screened on the new RegisPack® CLA-1 column. Following are some chromatographic illustrations and a tabular summary of our results.
Figure 2: Chromatogram Set 2. Sample: Verapamil. Mobile Phase: CO2/IPA (75/25) + 0.5% DEA. Flow Rate: 4.0 mL/min. Temp: 40 °C. Pressure: 125 bar. UV: 290 nm.
Conclusion
The RegisPack® is a well-established CSP that does a superior job of separating the majority of compounds submitted for chiral screening. There are cases where the RegisPack® CSP can only achieve partial or no separation on some analytes. The new RegisPack® CLA-1 can exhibit unique selectivity in separation when existing CSP's fail. This CSP is highly recommended to be added to the screening process.
Note
All work was performed on a WATERS THAR SFC Method Station.
Regis Technologies, Inc.
8210 Austin Ave, Morton Grove, IL 60053
tel. (847) 583-7661, fax (847) 967-1214
E-mail: teds@registech.com, Website: www.registech.com/chiral
New Study Uses MSPE with GC–MS to Analyze PFCAs in Water
January 20th 2025Scientists from the China University of Sciences combined magnetic solid-phase extraction (MSPE) with gas chromatography–mass spectrometry (GC–MS) to analyze perfluoro carboxylic acids (PFCAs) in different water environments.
The Next Frontier for Mass Spectrometry: Maximizing Ion Utilization
January 20th 2025In this podcast, Daniel DeBord, CTO of MOBILion Systems, describes a new high resolution mass spectrometry approach that promises to increase speed and sensitivity in omics applications. MOBILion recently introduced the PAMAF mode of operation, which stands for parallel accumulation with mobility aligned fragmentation. It substantially increases the fraction of ion used for mass spectrometry analysis by replacing the functionality of the quadrupole with high resolution ion mobility. Listen to learn more about this exciting new development.
A Guide To Finding the Ideal Syringe and Needle
January 20th 2025Hamilton has produced a series of reference guides to assist science professionals in finding the best-suited products and configurations for their applications. The Syringe and Needle Reference Guide provides detailed information on Hamilton Company’s full portfolio of syringes and needles. Everything from cleaning and preventative maintenance to individual part numbers are available for review. It also includes selection charts to help you choose between syringe terminations like cemented needles and luer tips.
The Complexity of Oligonucleotide Separations
January 9th 2025Peter Pellegrinelli, Applications Specialist at Advanced Materials Technology (AMT) explains the complexity of oligonucleotide separations due to the unique chemical properties of these molecules. Issues such as varying length, sequence complexity, and hydrophilic-hydrophobic characteristics make efficient separations difficult. Separation scientists are addressing these challenges by modifying mobile phase compositions, using varying ion-pairing reagents, and exploring alternative separation modes like HILIC and ion-exchange chromatography. Due to these complexities, AMT has introduced the HALO® OLIGO column, which offers high-resolution, fast separations through its innovative Fused-Core® technology and high pH stability. Alongside explaining the new column, Peter looks to the future of these separations and what is next to come.
Oasis or Sand Dune? Isolation of Psychedelic Compounds
January 20th 2025Magic mushrooms, once taboo, have recently experienced a renaissance. This new awakening is partially due to new findings that indicate the effects of psilocybin, and its dephosphorylated cousin psilocin may produce long lasting results for patients who might be struggling with anxiety, depression, alcohol and drug abuse, and post-traumatic stress disorder. Hamilton Company has developed a methodology for the isolation and identification of 5 common psychedelic compounds used in the potential treatment of disease. The PRP-1 HPLC column resin remains stable in the harsh alkaline conditions ideal for better separations.
