Octadecyl bonded silica gel (C18) is widely used in solid-phase extraction (SPE), but there are many variations including particle size, pore size, surface chemistry, and carbon loading applications.
Whole blood analysis is an emerging trend in the field of bioanalysis. We developed a fast and simple protocol to extract and analyze a peptide, hepcidin, from whole blood. Sampling and extraction were carried out using volumetric absorptive microsampling (VAMS), a novel blood collection device that allows the sampling of a known blood volume independently from hematocrit. The composition of the extraction medium was optimized using an experimental design to get the most intense signal of hepcidin, considering different organic solvents and acidic additives.
Whole blood analysis is an emerging trend in the field of bioanalysis. We developed a fast and simple protocol to extract and analyze a peptide, hepcidin, from whole blood. Sampling and extraction were carried out using volumetric absorptive microsampling (VAMS), a novel blood collection device that allows the sampling of a known blood volume independently from hematocrit. The composition of the extraction medium was optimized using an experimental design to get the most intense signal of hepcidin, considering different organic solvents and acidic additives.
Whole blood analysis is an emerging trend in the field of bioanalysis. We developed a fast and simple protocol to extract and analyze a peptide, hepcidin, from whole blood. Sampling and extraction were carried out using volumetric absorptive microsampling (VAMS), a novel blood collection device that allows the sampling of a known blood volume independently from hematocrit. The composition of the extraction medium was optimized using an experimental design to get the most intense signal of hepcidin, considering different organic solvents and acidic additives.
Whole blood analysis is an emerging trend in the field of bioanalysis. We developed a fast and simple protocol to extract and analyze a peptide, hepcidin, from whole blood. Sampling and extraction were carried out using volumetric absorptive microsampling (VAMS), a novel blood collection device that allows the sampling of a known blood volume independently from hematocrit. The composition of the extraction medium was optimized using an experimental design to get the most intense signal of hepcidin, considering different organic solvents and acidic additives.
Whole blood analysis is an emerging trend in the field of bioanalysis. We developed a fast and simple protocol to extract and analyze a peptide, hepcidin, from whole blood. Sampling and extraction were carried out using volumetric absorptive microsampling (VAMS), a novel blood collection device that allows the sampling of a known blood volume independently from hematocrit. The composition of the extraction medium was optimized using an experimental design to get the most intense signal of hepcidin, considering different organic solvents and acidic additives.
A newly discovered method, matrix-assisted ionization (MAI), is described for generating gas-phase ions from volatile and nonvolatile compounds. The method is both simple and sensitive.
A newly discovered method, matrix-assisted ionization (MAI), is described for generating gas-phase ions from volatile and nonvolatile compounds. The method is both simple and sensitive.
A newly discovered method, matrix-assisted ionization (MAI), is described for generating gas-phase ions from volatile and nonvolatile compounds. The method is both simple and sensitive.
A newly discovered method, matrix-assisted ionization (MAI), is described for generating gas-phase ions from volatile and nonvolatile compounds. The method is both simple and sensitive.
A newly discovered method, matrix-assisted ionization (MAI), is described for generating gas-phase ions from volatile and nonvolatile compounds. The method is both simple and sensitive.
Jean-Luc Veuthey and Alexandre Grand-Guillaume-Perrenoud reveal the latest developments in supercritical fluid chromatography (SFC) that are bringing the technique back to the limelight.
A newly discovered method, matrix-assisted ionization (MAI), is described for generating gas-phase ions from volatile and nonvolatile compounds. The method is both simple and sensitive.
A newly discovered method, matrix-assisted ionization (MAI), is described for generating gas-phase ions from volatile and nonvolatile compounds. The method is both simple and sensitive.
The development of various analytical MS methods to investigate the chemical composition of liquids used in electronic cigarettes and characterize their quality is presented in this study.
The development of various analytical MS methods to investigate the chemical composition of liquids used in electronic cigarettes and characterize their quality is presented in this study.
The development of various analytical MS methods to investigate the chemical composition of liquids used in electronic cigarettes and characterize their quality is presented in this study.
The development of various analytical MS methods to investigate the chemical composition of liquids used in electronic cigarettes and characterize their quality is presented in this study.
The development of a microfabricated comprehensive GC that can be used as a portable measurement device.
High-resolution mass spectrometry (MS) is not readily compatible with chromatographic techniques using high-salt mobile phases. This study presents the development and use of 2D-LC–MS via an on-line desalting step for the analysis of monoclonal antibodies and antibody–drug conjugates.
Measuring trypsin inhibitors in legumes is important to feed processors, who are concerned with providing high-quality products for animal feed. In this study, a rapid, accurate, and precise method for the quantification of trypsin inhibitor activity is evaluated.
Bioinert and biocompatible liquid chromatography (LC) systems are becoming more commonplace in laboratories, but the majority of biomolecule separations still use LC systems composed primarily of stainless steel parts. Can passivation or mobile phase additives improve separations on these systems for metal-sensitive biomolecules?
A novel method to simultaneously characterize the capping and poly(A) tail for therapeutic mRNA in a single sample preparation workflow is described.
Among all the analytical techniques available for epitope mapping studies, hydrogen–deuterium exchange mass spectrometry (HDX-MS) is usually the fastest and easiest to carry out. We present here the epitope mapping of three distinct monoclonal antibody (mAb) candidates targeting the same antigen, an interleukin receptor. The goal is to establish the binding mode of these mAbs, and explain possible differences observed for in vitro binding and in vivo function.
High-resolution mass spectrometry (MS) is not readily compatible with chromatographic techniques using high-salt mobile phases. This study presents the development and use of 2D-LC–MS via an on-line desalting step for the analysis of monoclonal antibodies and antibody–drug conjugates.
Explore the potential for screening human blood fortified with ethylenediaminetetraacetic acid (EDTA) via LC/MS using an expression single quadrupole compact mass spectrometer.
Explore the potential for screening human blood fortified with ethylenediaminetetraacetic acid (EDTA) via LC/MS using an expression single quadrupole compact mass spectrometer.
With the growing use of illegal opioids, analysts should be prepared for a large influx of samples in their laboratories. While the workload may be increasing, the number of analysts and equipment may not, so the need for faster and better liquid chromatography–mass spectrometry (LC–MS) methods is important. By implementing an efficient sample preparation technique for matrix cleanup for some of the most common and traditional opioid matrices-blood and urine-coupled with a rapid and accurate LC method, laboratories can address the analytical needs for this growing problem.