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Whole-Column-Imaging Detection for Capillary Isoelectric Focusing and Capillary ElectrophoresisIn this article, the authors review recent developments in the research of whole-column-imaging detection for capillary electrophoresis (CE). Whole-column-imaging detection was developed for capillary isoelectric focusing, for which it proved to be an ideal detector. Several whole-column-imaging detectors -- including refractive index gradient imaging, UV-absorption imaging, and fluorescence imaging detectors -- have been studied. The capillary isoelectric focusing UV-absorption imaging technique even has been commercialized. The development of whole-column-imaging detection itself facilitates CE studies in many directions such as in electrophoretic dynamics within narrow channels, new separation modes, and two-dimensional separations. Whole-column-imaging detection also finds application in capillary zone electrophoresis.
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Embedded-Polar-Group Bonded Phases for High Performance Liquid ChromatographyThe authors compare the chromatographic properties of embedded-carbamate-group bonded phases with those of their purely alkyl counterparts.
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Embedded-Polar-Group Bonded Phases for High Performance Liquid ChromatographyThe authors compare the chromatographic properties of embedded-carbamate-group bonded phases with those of their purely alkyl counterparts.
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Embedded-Polar-Group Bonded Phases for High Performance Liquid ChromatographyThe authors compare the chromatographic properties of embedded-carbamate-group bonded phases with those of their purely alkyl counterparts.
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Embedded-Polar-Group Bonded Phases for High Performance Liquid ChromatographyThe authors compare the chromatographic properties of embedded-carbamate-group bonded phases with those of their purely alkyl counterparts.
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Preparation and Characterization of a Pyrethrum Extract StandardThe authors describe how they separated and collected milligram quantities of six pyrethrin esters in pyrethrum extract and used them to create analytical reference standards for these insecticide compounds.
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Buffers and BaselinesThis month's "LC Troubleshooting" focuses on the baseline characteristics of several common LC buffers and methods to minimize drift.
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Analysis of Weak UV-Absorbing Pharmaceutical Drug Substances Using CE with Condensation Nucleation Light-Scattering DetectionThe authors descibe the use of CE with condensation nucleation light-scattering detection to analyze three pharmaceutical analytes. Because the compounds lack adequate UV chromophores, they require a more sensitive means of detection.
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Preparation and Characterization of a Pyrethrum Extract StandardThe authors describe how they separated and collected milligram quantities of six pyrethrin esters in pyrethrum extract and used them to create analytical reference standards for these insecticide compounds.
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Analysis of Weak UV-Absorbing Pharmaceutical Drug Substances Using CE with Condensation Nucleation Light-Scattering DetectionThe authors descibe the use of CE with condensation nucleation light-scattering detection to analyze three pharmaceutical analytes. Because the compounds lack adequate UV chromophores, they require a more sensitive means of detection.
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Analysis of Weak UV-Absorbing Pharmaceutical Drug Substances Using CE with Condensation Nucleation Light-Scattering DetectionThe authors descibe the use of CE with condensation nucleation light-scattering detection to analyze three pharmaceutical analytes. Because the compounds lack adequate UV chromophores, they require a more sensitive means of detection.
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Analysis of Weak UV-Absorbing Pharmaceutical Drug Substances Using CE with Condensation Nucleation Light-Scattering DetectionThe authors descibe the use of CE with condensation nucleation light-scattering detection to analyze three pharmaceutical analytes. Because the compounds lack adequate UV chromophores, they require a more sensitive means of detection.
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The Story of Thin-Layer ChromatographyThis installment surveys the evolution of thin-layer chromatography...
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Buffers and BaselinesThis month's "LC Troubleshooting" focuses on the baseline characteristics of several common LC buffers and methods to minimize drift.
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Ultrahigh-Efficiency Protein Separations with Submicrometer Silica Using Slip FlowProfessor Mary Wirth and graduate students Bingchuan Wei and Benjamin Rogers from Purdue University demonstrate a quantum leap in protein column efficiency. Using colloidal silica particles of submicrometer diameters (470 nm), they obtained plate heights that were as much as 15-fold lower than the theoretical limit for Hagen-Poiseuille flow.
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Ultrahigh-Efficiency Protein Separations with Submicrometer Silica Using Slip FlowProfessor Mary Wirth and graduate students Bingchuan Wei and Benjamin Rogers from Purdue University demonstrate a quantum leap in protein column efficiency. Using colloidal silica particles of submicrometer diameters (470 nm), they obtained plate heights that were as much as 15-fold lower than the theoretical limit for Hagen-Poiseuille flow.
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Selective Sorbents for Solid-Phase Extraction Based on Molecularly Imprinted PolymersHighly selective sorbents for solid-phase extraction (SPE) are desirable tools for the development of selective and sensitive methods for trace analysis. Molecular imprinting is an emerging technique that can yield analyte- or group-selective polymeric sorbents.
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Advances in Amino Acid AnalysisThe authors review current strategies for amino acid analysis and explain their novel approach for simplified sample preparation of body fluids for amino acid analysis...
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Advances in Amino Acid AnalysisThe authors review current strategies for amino acid analysis and explain their novel approach for simplified sample preparation of body fluids for amino acid analysis...
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Detecting Mushroom Peptide Toxins in Body Fluids by Capillary ElectrophoresisThe Death Cap mushroom is the cause of most mushroom-related poisonings in the world. The author has developed a highly efficient, sensitive CE technique that toxicologists and forensic analysts can use to determine the poisonous peptides in body fluids of affected patients.
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Capillary Electrophoresis as a Routine Analytical Tool in Pharmaceutical AnalysisThis "CE Currents" column examines the use of capillary electrophoresis (CE) in three important areas of pharmaceutical analysis.
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Capillary Electrophoresis as a Routine Analytical Tool in Pharmaceutical AnalysisThis "CE Currents" column examines the use of capillary electrophoresis (CE) in three important areas of pharmaceutical analysis.
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Selective Sorbents for Solid-Phase Extraction Based on Molecularly Imprinted PolymersHighly selective sorbents for solid-phase extraction (SPE) are desirable tools for the development of selective and sensitive methods for trace analysis. Molecular imprinting is an emerging technique that can yield analyte- or group-selective polymeric sorbents.
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Striving to Validate NutraceuticalsGuest authors Thompson and Morris summarize the problems and progress in establishing regulated analytical methods for dietary supplements.
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Striving to Validate NutraceuticalsGuest authors Thompson and Morris summarize the problems and progress in establishing regulated analytical methods for dietary supplements.
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New Developments in the Application of Monolithic HPLC ColumnsThe guest authors discuss the use of monolithic silica columns in high-throughput HPLC, including developments and applications in combinatorial chemistry. They also explain performance characteristics for these columns and provide caveats about their effective usage.
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New Developments in the Application of Monolithic HPLC ColumnsThe guest authors discuss the use of monolithic silica columns in high-throughput HPLC, including developments and applications in combinatorial chemistry. They also explain performance characteristics for these columns and provide caveats about their effective usage.
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New Developments in the Application of Monolithic HPLC ColumnsThe guest authors discuss the use of monolithic silica columns in high-throughput HPLC, including developments and applications in combinatorial chemistry. They also explain performance characteristics for these columns and provide caveats about their effective usage.
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New Developments in the Application of Monolithic HPLC ColumnsThe guest authors discuss the use of monolithic silica columns in high-throughput HPLC, including developments and applications in combinatorial chemistry. They also explain performance characteristics for these columns and provide caveats about their effective usage.
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Analytica 2024A look at some of the highlights that separation scientists can look forward to at Analytica 2024.
