Author
Latest:
Evaluating the Isolation and Quantification of Sterols in Seed Oils by Solid-Phase Extraction and Capillary Gas–Liquid ChromatographyThe authors describe two tests performed using SPE. The first involved sterol isolation and cleanup of seed oils, and the second analyzed crude seed oils.
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Hybrid Organic–Inorganic Particle Technology: Breaking Through Traditional Barriers of HPLC SeparationsThe authors describe reversed-phase HPLC based on hybrid particles and demonstrate the packing's retention and selectivity characteristics.
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Hybrid Organic–Inorganic Particle Technology: Breaking Through Traditional Barriers of HPLC SeparationsThe authors describe reversed-phase HPLC based on hybrid particles and demonstrate the packing's retention and selectivity characteristics.
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Embedded-Polar-Group Bonded Phases for High Performance Liquid ChromatographyThe authors compare the chromatographic properties of embedded-carbamate-group bonded phases with those of their purely alkyl counterparts.
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Hybrid Organic–Inorganic Particle Technology: Breaking Through Traditional Barriers of HPLC SeparationsThe authors describe reversed-phase HPLC based on hybrid particles and demonstrate the packing's retention and selectivity characteristics.
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Modifying the Metal Surfaces in HPLC Systems and Columns to Prevent Analyte Adsorption and Other Deleterious EffectsA novel surface modification technology has been developed to reduce interactions between analytes and metal surfaces in HPLC instruments and columns. We demonstrate the impact of this technology on peak symmetry, peak area, and injection-to-injection and column-to-column reproducibility for several metal-sensitive analytes.
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Reproducibility and Carryover — A Case StudyThis month's column looks at reproducibility and carryover problems associated with the development of a method used to verify analyst proficiency.
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Hybrid Organic–Inorganic Particle Technology: Breaking Through Traditional Barriers of HPLC SeparationsThe authors describe reversed-phase HPLC based on hybrid particles and demonstrate the packing's retention and selectivity characteristics.
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Validating a Reversed-Phase HPLC Method to Determine Residual Nonoxynol-9 on Pharmaceutical Process Equipment Using a Nonporous Silica ColumnThe authors' laboratory developed and validated an HPLC method to test for residual nonoxynol-9 on stainless steel, PTFE, and acrylic substrates.
Latest:
Validating a Reversed-Phase HPLC Method to Determine Residual Nonoxynol-9 on Pharmaceutical Process Equipment Using a Nonporous Silica ColumnThe authors' laboratory developed and validated an HPLC method to test for residual nonoxynol-9 on stainless steel, PTFE, and acrylic substrates.
Latest:
Embedded-Polar-Group Bonded Phases for High Performance Liquid ChromatographyThe authors compare the chromatographic properties of embedded-carbamate-group bonded phases with those of their purely alkyl counterparts.
Latest:
Validating a Reversed-Phase HPLC Method to Determine Residual Nonoxynol-9 on Pharmaceutical Process Equipment Using a Nonporous Silica ColumnThe authors' laboratory developed and validated an HPLC method to test for residual nonoxynol-9 on stainless steel, PTFE, and acrylic substrates.
Latest:
Hybrid Organic–Inorganic Particle Technology: Breaking Through Traditional Barriers of HPLC SeparationsThe authors describe reversed-phase HPLC based on hybrid particles and demonstrate the packing's retention and selectivity characteristics.
Latest:
Validating a Reversed-Phase HPLC Method to Determine Residual Nonoxynol-9 on Pharmaceutical Process Equipment Using a Nonporous Silica ColumnThe authors' laboratory developed and validated an HPLC method to test for residual nonoxynol-9 on stainless steel, PTFE, and acrylic substrates.
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Implementing a Generic Method Development Strategy for Enantiomer AnalysisAuthors Chapman and Chen describe an enantiomer analysis method development strategy that uses a family of highly sulfated cyclodextrins to resolve a diverse array of compounds.
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Practical Aspects of Mucopolysaccharidoses Screening with Thin-Layer ChromatographyScored layers, sequential development, modifications of the development chamber, and a careful choice of standards produce better reliability in thin-layer chromatography analysis of urine for the diagnosis of mucopolysaccharidoses. In equivocal cases, repeating the procedure and varying the solvents will improve results.
Latest:
Implementing a Generic Method Development Strategy for Enantiomer AnalysisAuthors Chapman and Chen describe an enantiomer analysis method development strategy that uses a family of highly sulfated cyclodextrins to resolve a diverse array of compounds.
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Determining Organophosphorus Pesticides in Foods Using Accelerated Solvent Extraction with Large Sample SizesThe authors determined the presence of 26 organophosphorus pesticides in apple and carrot samples using accelerated solvent extraction.
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Practical Aspects of Mucopolysaccharidoses Screening with Thin-Layer ChromatographyScored layers, sequential development, modifications of the development chamber, and a careful choice of standards produce better reliability in thin-layer chromatography analysis of urine for the diagnosis of mucopolysaccharidoses. In equivocal cases, repeating the procedure and varying the solvents will improve results.
Latest:
Practical Aspects of Mucopolysaccharidoses Screening with Thin-Layer ChromatographyScored layers, sequential development, modifications of the development chamber, and a careful choice of standards produce better reliability in thin-layer chromatography analysis of urine for the diagnosis of mucopolysaccharidoses. In equivocal cases, repeating the procedure and varying the solvents will improve results.
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Microanalysis of D/L-Amino Acid Residues in Peptides and ProteinsThis article describes the determination of D/L-amino acid residues in peptides at microgram levels.
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IC–MS Determination of Ionic Compounds in ToothpasteA fast and easy-to-use method for the identifcation and determination of active anionic ingredients in toothpaste is presented using ion chromatography in combination with electrospray mass spectrometry. In this application, positive identification of glycerol, sorbitol, chloride, fluoride, sulphate, monofluorophosphate and polyphospates, in addition to citrate and saccharin, is achieved in one chromatographic run with a total analysis time of 22 min.
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Microanalysis of D/L-Amino Acid Residues in Peptides and ProteinsThis article describes the determination of D/L-amino acid residues in peptides at microgram levels.
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Whole-Column-Imaging Detection for Capillary Isoelectric Focusing and Capillary ElectrophoresisIn this article, the authors review recent developments in the research of whole-column-imaging detection for capillary electrophoresis (CE). Whole-column-imaging detection was developed for capillary isoelectric focusing, for which it proved to be an ideal detector. Several whole-column-imaging detectors -- including refractive index gradient imaging, UV-absorption imaging, and fluorescence imaging detectors -- have been studied. The capillary isoelectric focusing UV-absorption imaging technique even has been commercialized. The development of whole-column-imaging detection itself facilitates CE studies in many directions such as in electrophoretic dynamics within narrow channels, new separation modes, and two-dimensional separations. Whole-column-imaging detection also finds application in capillary zone electrophoresis.
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Embedded-Polar-Group Bonded Phases for High Performance Liquid ChromatographyThe authors compare the chromatographic properties of embedded-carbamate-group bonded phases with those of their purely alkyl counterparts.
Latest:
Embedded-Polar-Group Bonded Phases for High Performance Liquid ChromatographyThe authors compare the chromatographic properties of embedded-carbamate-group bonded phases with those of their purely alkyl counterparts.
Latest:
Embedded-Polar-Group Bonded Phases for High Performance Liquid ChromatographyThe authors compare the chromatographic properties of embedded-carbamate-group bonded phases with those of their purely alkyl counterparts.
Latest:
Embedded-Polar-Group Bonded Phases for High Performance Liquid ChromatographyThe authors compare the chromatographic properties of embedded-carbamate-group bonded phases with those of their purely alkyl counterparts.
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Preparation and Characterization of a Pyrethrum Extract StandardThe authors describe how they separated and collected milligram quantities of six pyrethrin esters in pyrethrum extract and used them to create analytical reference standards for these insecticide compounds.
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Buffers and BaselinesThis month's "LC Troubleshooting" focuses on the baseline characteristics of several common LC buffers and methods to minimize drift.
