Application Notes

The Column spoke to Carlos Calderón about his research project, investigating pharmacolipidomics analysis of betulin in human primary keratinocytes to monitor alterations in the lipid profiles induced by treatment with betulin.

Incognito offers some tips for chromatographers during the current crisis

Microfluidic lab-on-a-chip devices offer new solutions for analytical scientists. The Column spoke to Dietlev Belder from the University of Leipzig, Germany, about the latest developments in this evolving area of research.

In this study, we demonstrate routine characterization of biotherapeutics through LC–MS peptide mapping using the Thermo Scientific™ Orbitrap Exploris™ 240 LC–MS/MS.

Accelerating fundamental research using the Orbitrap Exploris 480 mass spectrometer

PharmaFluidics’ μPAC capLC is evaluated for different user needs ranging from 12 samples per day in comprehensive mode to 65 samples per day in throughput mode.

PharmaFluidics introduces a new μPAC capLC column that offers unprecedented performance and that given its low backpressure can be used with flow rates of up to 15μL/min.

PharmaFluidics’ μPAC capLC is evaluated for different user needs ranging from 12 samples per day in comprehensive mode to 65 samples per day in throughput mode.

Overall gain in performance and increase in detection sensitivity that can be achieved for the separation of plant metabolite extracts by using µ¬PAC columns.

PharmaFluidics also offers a 50 cm long μPAC column which is perfectly suited to perform more throughput analyses with shorter gradient solvent times.

Analyzing skin ceramides using PharmaFluidics’ µPAC column technology reveals a substantial structural diversity and isomeric resolved sphingolipids.

Monitoring the excellent separation performance of PharmaFluidics’ µPAC columns by using peptide retention time calibration mixtures for bottom-up proteomics.

PharmaFluidics’ μPAC columns have the potential to be less prone to sample related column failure and to withstand more sample injections without losing performance.

Today’s conference highlights

Click the title above to open the LCGC Europe June 2020 regular issue, Volume 33, Number 6, in an interactive PDF format.

Abuse of synthetic opioid prescription painkillers such as fentanyl, along with a rapidly growing list of illicit analogues, is a significant public health problem. In this study, we developed a simple dilute-and-shoot method that provides a fast 3.5-min analysis of fentanyl and related compounds (norfentanyl, acetyl fentanyl, alfentanil, butyryl fentanyl, carfentanil, remifentanil, and sufentanil) in human urine by liquid chromatography–tandem mass spectrometry (LC–MS/ MS) using a Raptor Biphenyl column.

Monitoring the size heterogeneity of AAV-based gene therapy therapeutics is potentially important to ensure consistent product quality and efficacy. We demonstrate that the levels of both high-molecular weight (HMW) and low-molecular weight (LMW) impurities in AAV capsid preparations can be separated on a Waters 450 Å Protein BEH SEC column for a series of AAV serotypes.

Pharmaceutical companies worldwide are currently searching for vaccines, therapeutic agents, and prophylactics to combat COVID-19. The focus on biologic drugs during the current pandemic highlights the crucial role amino acids analysis plays in research and production of pharmaceuticals.

The single-stranded DNA (ssDNA) content of AAV capsids in AAV-based gene therapy preparations impacts the efficacy of this treatment modality. We demonstrate that AAV8 capsids without and with full length ssDNA can be separated and their relative abundances determined on a Waters Protein-Pak Hi Res Q strong anion-exchange column.

Dow scientists improved their method-development workflow by upgrading their hardware and using specialized software.

Reveal More in your Food. Using QSight LC–MS/MS to get a fast, selective, and sensitive multi-residue method for the determination of 136 pesticides in Pepper.

In this study we demonstrate the benefits of the Thermo Scientific™ Orbitrap Exploris™ 240 LC-MS/MS for intact biopharmaceutical analysis under native conditions.