Application Notes

Why water quality matters Improved weighing accuracy that conforms to regulations How filtration impacts your HPLC results

Comprehensive physicochemical characterization of biopolymers is accomplished by adding multi-angle light scattering and differential viscometry to standard SEC.

Branching impacts polymer properties such as rheology and stiffness. This note explains the analysis of branching ratio versus molar mass, accomplished by SEC-MALS(-IV).

H2O2 is often used as oxidizing agent in toothpaste. A fast and sensitive method is presented to allow the quantitation of hydrogen peroxide in less than 3 min.

EPA Method 552.3 is approved for the monitoring of HAAs System configuration allows ID, quantification, and confirmation in one run Results exceeded EPA quality assurance requirements

This note using YMC-Triart Bio C4 shows a robust separation at high temperatures for three commercially available mAbs: ofatumumab, palivizumab, and panitumumab.

This application note demonstrates the performance of an IC-MS/MS workflow for the analysis of polar pesticides in compliance with EU MRLs and EU SANTE guidelines.

This application note presents the role of ultrapure water in HPLC analysis.

This application note demonstrates the use of high-temperature headspace sampling as a surrogate vaporization environment to evaluate e-juice thermal degradation.

* Novel balance systems: next step toward lab automation? * Trends in sample prep * New lab weighing technology

-Use of smaller and sub-2-µm superficially porous particles for LC separations -Adoption of LC and SPP in cannabis testing -Metal–organic frameworks for liquid chromatography

The Column spoke to Jelle De Vos from the Department of Chemical Engineering at the Vrije Universiteit, in Brussels, Belgium, about his work investigating the performance of columns packed with 1.7-μm particles at high-pressure conditions (ΔPmax = 1034 bar) and linking it to particle and column-bed integrity.

A review of the 13th International Symposium on Packed Column SFC and SFE Techniques (SFC 2019), which was held in Philadelphia, Pennsylvania, USA, on 29 September–1 October 2019.

-483 citations and data integrity -FDA’s Data Integrity Guidance -Weighing in pharmaceutical and food labs

Mobile phase flow is critically important, and must be carefully controlled. In this instalment of “GC Connections”, we discuss carrier gas flow and its importance in successful gas chromatographic (GC) analysis. We will begin with a short review of fundamental theories showing why flow is important, and move into a discussion of how flow rate is measured and controlled on modern instruments. Finally, we will discuss the effect of flow-related parameters, including carrier gas choice, the difference between constant pressure and constant flow modes, and some new thinking about flow rate optimization. We conclude with some takeaways that should assist gas chromatographers with effective carrier gas management.

The effect of carrier liquid composition on the molecular properties of uncross-linked and enzymatically cross-linked caseinate solutions was studied by asymmetrical flow field-flow fractionation (AF4).

How do bioinert and biocompatible liquid chromatography (LC) systems and columns improve separations of biomolecules? How do I know when these systems are required for my separation?

Pesticides are widely used to protect agricultural products from mould or insect infestation during growth, storage, and product transport. Intensive use or abuse of pesticides can cause environmental pollution in different areas. Lakes, rivers, and aquifers need to be monitored to protect drinking water. Concentration levels of pesticides down to low fg/μL range must therefore be quantified in organic solvent-based water extracts. Typically, large volume injections (LVI) are used to reach this concentration range.

Amylose is an occasionally branched biopolymer and, together with amylopectin, the hyper-branched component, a constituent of starch. Determination of branching in amylopectin, an important property affecting viscosity, stiffness, and thermo-mechanical performance, requires a fully linear amylose analogue. This application note describes how synthetic amyloses from enzymatic (phosphorolytic) reaction were checked for their linearity using size-exclusion chromatography-multi-angle light scattering (SEC-MALS).

Antisense DNA and siRNA are widely used for gene silencing in research and medical applications. An effective delivery of the oligonucleotides into cells is important for clinical applications.

This application note demonstrates the transfer of analytical HPLC methods from an Agilent 1260 Infinity LC system to the Vanquish Core HPLC system.

This application note demonstrates the transfer of analytical HPLC methods from a Waters Alliance HPLC system to the Vanquish Core HPLC system.

The molecular similarity between Humira® and Yervoy® biosimilars and the corresponding innovator reference products is examined using analytical SEC, RPC and HIC.

· Traps and “delays” system-related PFAS, preventing interference · Allows accurate identification and quantitation of trace-level PFAS · Highly retentive-no breakthrough even with extended equilibration times

-High-efficiency source in GC/MS: using sensitivity to enhance productivity -Simultaneous analysis of volatile organic compounds and 1,4-dioxane -Industry challenges and the importance of reliable, robust technology

A focus on research scientist and analytical chemist, Diana Jaeger, analyzing samples using LC/TQ for River Stone Biotech, a new player in synthetic biology.

A simple and sensitive method is presented for the direct analysis of Glyphosate and AMPA in drinking water without sample derivatization or pre-concentration.

This application note outlines a simple SPE procedure for the extraction of 26 diverse per- and polyfluoroalkyl substances (PFASs) in drinking water.

This white paper discusses the advantages of using fast, efficient HALO® Fused-Core® Technology columns and manipulating selectivity to quickly develop methods, for fast high efficiency LC and LCMS separations.