Application Notes

Monitoring the excellent separation performance of PharmaFluidics’ µPAC columns by using peptide retention time calibration mixtures for bottom-up proteomics.

PharmaFluidics’ μPAC columns have the potential to be less prone to sample related column failure and to withstand more sample injections without losing performance.

Today’s conference highlights

Click the title above to open the LCGC Europe June 2020 regular issue, Volume 33, Number 6, in an interactive PDF format.

Abuse of synthetic opioid prescription painkillers such as fentanyl, along with a rapidly growing list of illicit analogues, is a significant public health problem. In this study, we developed a simple dilute-and-shoot method that provides a fast 3.5-min analysis of fentanyl and related compounds (norfentanyl, acetyl fentanyl, alfentanil, butyryl fentanyl, carfentanil, remifentanil, and sufentanil) in human urine by liquid chromatography–tandem mass spectrometry (LC–MS/ MS) using a Raptor Biphenyl column.

Monitoring the size heterogeneity of AAV-based gene therapy therapeutics is potentially important to ensure consistent product quality and efficacy. We demonstrate that the levels of both high-molecular weight (HMW) and low-molecular weight (LMW) impurities in AAV capsid preparations can be separated on a Waters 450 Å Protein BEH SEC column for a series of AAV serotypes.

Pharmaceutical companies worldwide are currently searching for vaccines, therapeutic agents, and prophylactics to combat COVID-19. The focus on biologic drugs during the current pandemic highlights the crucial role amino acids analysis plays in research and production of pharmaceuticals.

The single-stranded DNA (ssDNA) content of AAV capsids in AAV-based gene therapy preparations impacts the efficacy of this treatment modality. We demonstrate that AAV8 capsids without and with full length ssDNA can be separated and their relative abundances determined on a Waters Protein-Pak Hi Res Q strong anion-exchange column.

Dow scientists improved their method-development workflow by upgrading their hardware and using specialized software.

Reveal More in your Food. Using QSight LC–MS/MS to get a fast, selective, and sensitive multi-residue method for the determination of 136 pesticides in Pepper.

In this study we demonstrate the benefits of the Thermo Scientific™ Orbitrap Exploris™ 240 LC-MS/MS for intact biopharmaceutical analysis under native conditions.

In this study, we demonstrate routine characterization of biotherapeutics through LC–MS peptide mapping using the Thermo Scientific™ Orbitrap Exploris™ 240 LC–MS/MS.

Retention-time prediction (RT) could hasten the identification of unknowns. See how scientists use prediction software to estimate the RT of environmental contaminants.

DAICEL’s CHIRALPAK® sub-2 µm columns yield sub 30-second chiral separations of Thalidomide and Naproxen under SFC conditions.

This application note provides quantitative analysis of δ9-THC from cannabidiol oil using a pyroprobe interfaced to a GC–MS.

This note looks at the analysis of N-nitrosamines in rubber teats, soothers, and toys, with specific focus on babies and young children under UK method BS EN 71-12:2016.

Reveal more in your food. Using QSight LC–MS/MS to get a fast, selective, and sensitive multi-residue method for the determination of 136 pesticides in pepper.

Isocratic HPLC method for the analysis of mycotoxins and aflatoxins uses the DFL-10 filter fluorescence detector with a photochemical reactor to improve sensitivity.

-Why ultrafiltration is important in chromatography -Applications for ultrafiltration -Using Vivaspins® for sample clarification

Method development for simultaneous analysis of ultrashort-chain, alternative, and legacy per- and polyfluoroalkyl substances (PFAS).

Method Development for Simultaneous Analysis of Ultrashort-Chain, Alternative, and Legacy per- and polyfluoroalkyl substances (PFAS).