Application Notes

Environmental laboratories analyze customer samples to determine the concentration of contaminants in soil, drinking water, and so on. Frequently these samples are diluted prior to analysis by the ICP-MS. It is critical that these dilutions are accurate and reproducible. In this study we compared the speed, accuracy, and consumable costs for common sample preparation techniques and found that the Microlab 600 is the best option.

Smaller particle size stationary phases have seen increased use in the modern analytical laboratory due to their ability to exhibit greater resolution while enabling the use of smaller column geometries. Shorter column lengths and narrower bores allow for faster run times and lower flow rates, decreasing laboratory costs in terms of solvent consumption and man-hours needed to run the analyses. This application note examines the effects of smaller particle sizes in various column geometries on size-exclusion chromatography (SEC) methods and the method variables affecting the characterization of antibody products.

Decrease analysis time for fluorochemicals with no sacrifice in chromatographic performance. Rapid perfluorinated alkyl analysis by LC-MS/MS increases sample throughput.

The Agilent 1290 Infinity II UHPLC, an Agilent 6545 Q-TOF-MS, and Agilent MassHunter BioConfirm B.08.00 software was used for accurate disulphide bond mapping.

An isocratic HPLC method showing the separation of an artificial sweetener in a cola beverage. The chromatogram shows the advantage of using 230nm instead of 254nm for improved detection but minimizing the baseline interferences.

This work focused on scaling-down a monoclonal antibody SEC method, starting with a typical analytical-size SEC column (YMC-Pack Diol 5um, 300Å, 300x8.0mm) and ending with a small particle, shorter length, narrower-bore column (YMC-pack Diol 2um, 300Å, 150x4.6mm). The purpose of this experiment was to detail the differing performance characteristics exhibited by each column as physical properties were altered, one variable at a time.

In the United States, the increasing acceptance of medical and recreational cannabis at the state level has created a need for accurate, precise and efficient analysis of cannabinoids in marijuana flower, extracts, and formulated products.

The poster describes how different suppressors (MSM and MCS) work and mentions possible applications.

Chromatographic conditions were developed for a fast GC-MS glycol ether analysis on the Rxi®-1301Sil MS column. This cyanobased thin film column provides better resolution and faster run times than the thick film cyanopropylphenyl-type columns commonly used for speciation of the glycol ethers. The glycol ethers are high production volume industrial chemicals that often occur as complex mixtures of isomers. The Rxi®-1301Sil MS column is uniquely matched for the separation of these isomers while still producing narrow and symmetric peaks for the low molecular weight ethylene glycol ethers.

This poster presents an approach that couples a Particle-Into-Liquid-Sampler (PILS) to a dual-channel ion chromatograph (IC) for measurement of aerosol anions and cations and a voltammetric measuring stand (VA) to determine the heavy metals. Feasibility of the PILS-IC-VA online system was demonstrated by collecting aerosol samples in Herisau Switzerland, at defined time intervals; air pollution events were simulated by burning lead- and cadmium-coated sparklers.

The combination of ion chromatography (IC) and inductively coupled plasma mass spectrometry (ICP/MS) provides a rapid, reliable, and sensitive speciation analysis of wastewater-relevant free and complexed gadolinium compounds. IC-ICP/MS proceeds without costly sample preparation and provides important information on the supply, degradation, and fate of the contrast agents in the (waste)water. The method is also highly suitable for determining compounds containing gadolinium in biological matrixes such as urine or blood. Additionally, IC-ICP/MS is a powerful tool for monitoring inorganic and iodine-containing ionic oxidation by-products that form during ozonation of iodinated X-contrast media.

This poster looks at the possibility to modify the existing EPA Method to meet California's rigorous public health goal (PHG) of 0.02 µg/L. After optimizing instrument settings and method parameters, a method detection limit (MDL) of 0.01 µg/L is obtained.

This poster discusses results showing the influence of pH, temperature of the post-column reactor, eluent composition, and iodide concentration on the sensitivity of the triiodide method.

The Combustion IC system presented allows the automated determination of organic halogen and sulfur compounds in all flammable samples. Both combustion digestion, which is automatically controlled with a flame sensor, and the professional Liquid Handling guarantee highest precision and trueness. This poster describes the determination of the halogen and sulfur content in a certified polymer standard, a coal reference material as well as in latex and vinyl gloves.

With the evolving market and demand for matrix-optimized transitions, the Agilent P&EP 4.0 Analyzer includes the addition of 7,800 matrix-optimized transitions to provide customers with their optimal pesticides analysis.

Method transfer or method scaling across different LC instruments can be affected by both dwell volume and extra-column dispersion. Each characteristic will have a different effect on the chromatographic separation.

With the increase of research with Eudragit, consistent molecular weight measurements have become more prevalent. Shodex has shown a repeatable SEC method using the GF-510 HQ column.

TSKgel® UP-SW3000 columns are 2 µm SEC columns designed for the analysis of monoclonal antibodies and other biopharma products and can be used on both HPLC and UHPLC systems. A TSKgel UP-SW3000 column yielded very low percent relative standard deviation (%RSD) for peak parameters including retention times, peak asymmetry, and efficiency, demonstrating the exceptional reproducibility of this column versus a competitor UHPLC column.

The characterization of monoclonal antibodies is a major challenge in process monitoring and quality control. TSKgel® G3000SWXL columns have been the industry standard for quality control of mAbs by SEC for decades. With the introduction of TSKgel UP-SW3000, 2 µm silica-based UHPLC–HPLC columns, increased speed and higher resolution can be achieved for the separation of antibody fragments, monomers, and dimers.

This application note discusses the use of a 3 μm particle size, 30 nm pore size, size exclusion chromatography (SEC) column for monitoring folded (native) and unfolded (denatured) states of a monoclonal antibody using fluorescence detection (FLD).

195 pesticides were chosen to evaluate both the traditional time segment (TS) acquisition and dMRM acquisition structures. The use of dMRM provides the capability to better tackle large multi-analyte analysis.

This application note shows how the combination of column chromatography (SEC/GPC) with Multi-Angle Light Scattering (MALS), Dynamic Light Scattering (DLS) and RI detection can be applied as a powerful tool to identify monomers, dimers, hexamers and higher aggregates of insulin.

This application note shows how the combination of column chromatography (SEC/GPC) with Multi-Angle Light Scattering (MALS), Dynamic Light Scattering (DLS) and RI detection can be applied as a powerful tool to identify monomers, dimers, hexamers and higher aggregates of insulin.

Traditional extraction typically involve intensive manual LLE shakes at both pH 2 and 12 prone heavy emulsions and low recoveries or CLLE for traditional base, neutral, and acid for semivolatiles extractions can take up to 24hrs. By incorporating automated SPE with multi-bed sorbents, base, neutral, and acid for semivolatiles samples can be extracted with a single pass procedure without emulsions, centrifuging, and hours of manual labor. The FMS TurboTrace ABN SPE system is designed to handle multiple cartridges and provides a fully automated solution for the semivolatile extraction process.

Traditional extraction typically involve intensive manual LLE shakes at both pH 2 and 12 prone heavy emulsions and low recoveries or CLLE for traditional base, neutral, and acid for semivolatiles extractions can take up to 24hrs. By incorporating automated SPE with multi-bed sorbents, base, neutral, and acid for semivolatiles samples can be extracted with a single pass procedure without emulsions, centrifuging, and hours of manual labor. The FMS TurboTrace ABN SPE system is designed to handle multiple cartridges and provides a fully automated solution for the semivolatile extraction process.

This application note demonstrates that Markes International’s automated thermal desorption systems offer excellent results for monitoring volatile organic compounds (VOCs) in stationary source emissions in accordance with the updated version of the European standard method CEN/TS 13649 released in 2014. The value of repeat analysis for method development and result verification is also demonstrated.