Application Notes

The advantage of UV spectroscopy as an analytical method to detect protein aggregation is that it is non-destructive and uses low sample volumes, minimal sample preparation requirement and easy sample analysis. A quick QC method to indicate the presence of aggregation uses an Agilent Cary 60 UV-Vis spectrophotometer to identify aggregation of mAb solutions resulting from different stress conditions.

Analytically, OPPs can pose some challenges due to the wide diversity of physical structures and behavioral characteristics of individual analytes. This often results in individual extraction protocols being implemented for labs to meet regulatory compliances. With the FMS, Inc TurboTrace® ABN Multi Cartridge SPE system, and its ability to incorporate dual cartridge extractions, it is possible to pair analytes with dissimilar characteristics into a single, fully automated extraction process.

Analytically, OPPs can pose some challenges due to the wide diversity of physical structures and behavioral characteristics of individual analytes. This often results in individual extraction protocols being implemented for labs to meet regulatory compliances. With the FMS, Inc TurboTrace® ABN Multi Cartridge SPE system, and its ability to incorporate dual cartridge extractions, it is possible to pair analytes with dissimilar characteristics into a single, fully automated extraction process.

With the recent legalization of cannabis in several states, there is a growing need for robust, reliable, and cost-effective analytical methods to facilitate routine testing for potency and contaminants. Here we present an LC–MS/MS method that uses the budget-friendly SCIEX Triple Quad 3500 LC–MS/MS system for the simultaneous detection and quantification of cannabinoids, pesticides, and mycotoxins in cannabis products using the Scheduled MRM algorithm.

Drinking water is an important part of environmental exposure, especially for small children. Countries around the world have put regulations in place to monitor drinking water quality for a wide range of hazardous compounds.

High resolution mass spectrometry with nano-LC is used for protein identification and quantification in both top-down and bottom-up proteome analysis. Reliable instrumentation in combination with ultrapure mobile phases is essential for data integrity. Premixed 80% acetonitrile with 0.1% formic acid (LS122-500) and water with 0.1% formic acid (LS118-500) were designed to produce a consistent chromatographic performance using this instrument system. In this study, these mobile phases were used extensively to evaluate several factors which can affect separation of protein digests such as peak retention, peak repeatability, and sample carryover. Our results demonstrated excellent chromatographic performance using Thermo Scientific EASY-nLC 1200 LC system and Thermo Scientific LTQLX ™ ion trap mass spectrometer with the specialized premixed mobile phases.

By switching to a Raptor C18 column, labs can process more samples per hour while still meeting fluorochemical method requirements.

Determination of PBDE congeners can be performed at high sensitivity with good accuracy using the GCMS-TQ8050.