Application Notes

With the increase of research with Eudragit, consistent molecular weight measurements have become more prevalent. Shodex has shown a repeatable SEC method using the GF-510 HQ column.

TSKgel® UP-SW3000 columns are 2 µm SEC columns designed for the analysis of monoclonal antibodies and other biopharma products and can be used on both HPLC and UHPLC systems. A TSKgel UP-SW3000 column yielded very low percent relative standard deviation (%RSD) for peak parameters including retention times, peak asymmetry, and efficiency, demonstrating the exceptional reproducibility of this column versus a competitor UHPLC column.

The characterization of monoclonal antibodies is a major challenge in process monitoring and quality control. TSKgel® G3000SWXL columns have been the industry standard for quality control of mAbs by SEC for decades. With the introduction of TSKgel UP-SW3000, 2 µm silica-based UHPLC–HPLC columns, increased speed and higher resolution can be achieved for the separation of antibody fragments, monomers, and dimers.

This application note discusses the use of a 3 μm particle size, 30 nm pore size, size exclusion chromatography (SEC) column for monitoring folded (native) and unfolded (denatured) states of a monoclonal antibody using fluorescence detection (FLD).

195 pesticides were chosen to evaluate both the traditional time segment (TS) acquisition and dMRM acquisition structures. The use of dMRM provides the capability to better tackle large multi-analyte analysis.

This application note shows how the combination of column chromatography (SEC/GPC) with Multi-Angle Light Scattering (MALS), Dynamic Light Scattering (DLS) and RI detection can be applied as a powerful tool to identify monomers, dimers, hexamers and higher aggregates of insulin.

This application note shows how the combination of column chromatography (SEC/GPC) with Multi-Angle Light Scattering (MALS), Dynamic Light Scattering (DLS) and RI detection can be applied as a powerful tool to identify monomers, dimers, hexamers and higher aggregates of insulin.

Traditional extraction typically involve intensive manual LLE shakes at both pH 2 and 12 prone heavy emulsions and low recoveries or CLLE for traditional base, neutral, and acid for semivolatiles extractions can take up to 24hrs. By incorporating automated SPE with multi-bed sorbents, base, neutral, and acid for semivolatiles samples can be extracted with a single pass procedure without emulsions, centrifuging, and hours of manual labor. The FMS TurboTrace ABN SPE system is designed to handle multiple cartridges and provides a fully automated solution for the semivolatile extraction process.

Traditional extraction typically involve intensive manual LLE shakes at both pH 2 and 12 prone heavy emulsions and low recoveries or CLLE for traditional base, neutral, and acid for semivolatiles extractions can take up to 24hrs. By incorporating automated SPE with multi-bed sorbents, base, neutral, and acid for semivolatiles samples can be extracted with a single pass procedure without emulsions, centrifuging, and hours of manual labor. The FMS TurboTrace ABN SPE system is designed to handle multiple cartridges and provides a fully automated solution for the semivolatile extraction process.

This application note demonstrates that Markes International’s automated thermal desorption systems offer excellent results for monitoring volatile organic compounds (VOCs) in stationary source emissions in accordance with the updated version of the European standard method CEN/TS 13649 released in 2014. The value of repeat analysis for method development and result verification is also demonstrated.

What is thermal desorption and how can it extend the capability of GC? In this podcast, you’ll hear the answers to these questions directly from Elizabeth Woolfenden, a leading expert on this sample introduction technique. As a co-founder of Markes International, widely acknowledged as the world leaders in TD, Elizabeth has been right at the heart of the development of thermal desorption, and will draw on her experience to highlight the scope of the technique, along with key technical innovations and emerging applications. So whether you’re new to TD and want a quick introduction, or an experienced analyst interested in hearing a personal perspective, don’t miss this unique opportunity to get the lowdown on thermal desorption from a major player in the area!

This application note describes a UPLC-MSE analysis of monoclonal antibody (mAb) disulfide bond. This study demonstrates that UNIFI-a compliance-ready software-is designed to support biopharmaceutical laboratories in performing fast peptide mapping and monitoring of mAb disulfide linkages.

This whitepaper provides a summary of the impact of PIC/S, and compliance with the PIC/S document PE 009-12 “Guide to Good Manufacturing Practice for Medicinal Products Annexes“ Annex 11 Computerised Systems. It includes a high-level foundation to understand how pharmaceutical manufacturing companies are being regulated, what PIC/S means for common requirements across different countries, and how these requirements relate to expectations for data from computerised systems/electronic records.

Pain management LC analyses can be difficult to optimize due to the limited selectivity of C18 and phenyl-hexyl phases. In contrast, the selectivity of Raptor Biphenyl superficially porous particle (SPP) LC columns provides complete resolution of isobaric pain medications with a total cycle time of 5 min.

Successful separations of the enantiomers of 3,3’-substituted BINOLs and their corresponding protected derivatives were obtained on the RegisPack® chiral stationary phase (CSP) from Regis Technologies, Inc.

This Application Note proves equivalency of the Agilent 1200 Series LC and the Agilent 1260 Infinity II LC for the analysis of tricyclic antidepressant drugs.

This Application Note proves equivalency of the Agilent 1200 Series LC and the Agilent 1260 Infinity II LC for the analysis of tricyclic antidepressant drugs.

Analysis of arsenic species is made challenging due to diverse sample matrices. To circumvent these problems, an HPLC ICP-MS method has been developed. Resolution of arsenic compounds is achieved by ion exchange chromatography on a Hamilton PRP-X100 column, a 55% cross-linked polystyrene-divinylbenzene copolymer functionalized with quaternary ammonium anion-exchanger group.