Application Notes

Microwave extraction becomes faster and easier with the new Milestone ETHOS X with its FastEX-24. This application note shows the use of Milestone technology for extraction of dioxins from environmental matrices, with emphasis on one of the most common challenge of this application: the carryover effect. The unique design of the FastEX-24 rotor with disposable glass vials allows easy and efficient dioxin extraction, and other organic pollutants, to be performed, avoiding any memory effect. The FastEX-24 simplifies the routine pollutants extraction process and provides superior productivity at lower costs.

A sensitive determination method with automated solid‑phase extraction (SPE) is presented and the recovery rates are compared with manual extraction. The automated extraction was developed with the SPE robot FREESTYLETM SPE module (LCTech GmbH). According to the European Regulation (EC) No 470/2009, CHROMABOND® HLB was applied for SPE (1). A high performance liquid chromatography tandem mass spectrometry (HPLC–MS/MS) method with the biphenyl phase NUCLEODUR® π2 was used for the subsequent analysis.

This study describes the monitoring of potentially harmful volatile organic compounds (VOCs) emitted from respiratory medical devices, by pumped sampling and thermal desorption–gas chromatography–mass spectrometry (TD–GC–MS) analysis in accordance with ISO 18562 part 3. Emissions from two sets of face-mask supply tubing and three nasal cannulas were compared, and all were found to emit VOCs at levels that may give cause for concern.

Methods for monitoring alcohol consumption biomarkers EtG and EtS are generally limited by poor retention and coelution with matrix interferences, as well as by long analysis times and short column lifetimes. The dilute-and-shoot EtG/EtS LC–MS/MS analysis developed here using the novel Raptor EtG/EtS column easily resolves EtG and EtS from matrix interferences, providing consistent, accurate results for high-throughput labs testing human urine samples for alcohol consumption.

This application note will present the data collected as part of the demonstration of disk solid phase extraction validation for US EPA method 625.1.

By switching to a Raptor C18 column, labs can process more samples per hour while still meeting fluorochemical method requirements.

This article describes a workflow using nontargeted liquid chromatography–tandem mass spectrometry (LC–MS/MS) for reliable compound identification.

The advantage of UV spectroscopy as an analytical method to detect protein aggregation is that it is non-destructive and uses low sample volumes, minimal sample preparation requirement and easy sample analysis. A quick QC method to indicate the presence of aggregation uses an Agilent Cary 60 UV-Vis spectrophotometer to identify aggregation of mAb solutions resulting from different stress conditions.