Jennifer M. Nguyen

A novel surface modification technology has been developed to reduce interactions between analytes and metal surfaces in HPLC instruments and columns. We demonstrate the impact of this technology on peak symmetry, peak area, and injection-to-injection and column-to-column reproducibility for several metal-sensitive analytes.

This article describes a new approach for measuring critical quality attributes including the confirmation of 5’ cap structure and 5’ capping efficiency.